PCR Purification kit is supplied by the company Aura Biotech.

In light of recent event pertaining to the global spread of the novel Corona Virus Disease (COVID-19), research units have been focussing a great deal of efforts on understanding the nature of the causative virus the novel SARS-CoV-2. Several laboratories have also been trying to develop vaccines and drugs against the virus. Diagnostic and clinical labs have been working on improving diagnostic techniques to increase the rate of testing of patient samples. In both diagnostics and research, PCR is a powerful tool which helps in the amplification of target genes. The amplified PCR products can be used for further downstream analysis. But first, the PCR product needs to be purified. The PCR Purification Kit from Aura Biotech is designed for efficient purification of PCR products. It utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labelled nucleotides, enzymes, and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 100 bp to 15 kb with a recovery rate of up to 100%. Each purification column has a total binding capacity of more than 10 g of DNA, and the entire procedure takes less than 10 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labelling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.

Product specifications:

• Brand: Aura Biotech
• Catalogue number: MPK4
• Quantity/unit: Pack of 20rxns

Product features:

• Efficiency: More than 90% recoveries in the range of 100 bp to 15 kb
• Speed: <10>