Auras PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.
The kit can be used for purification of DNA fragments from 100 bp to 15 kb with recovery rates up to 100%. Each purification column has a total binding capacity of more than 10 g of DNA, and the entire procedure takes less than 10 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.
Highly efficient: More than 90% recoveries in the range of 100 bp to 15 kb
Fast: procedure takes only less than 10 minutes
Convenient: spin columns are capped and assembled with collection tubes