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phi29 Polymerase  ,5000 U

phi29 Polymerase ,5000 U

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  • Brand: Blirt
  • Catalog No.: EN20-050
  • Quantity/Unit: 5000 U /bottle

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phi29 Polymerase ,5000 U supplied by BLIRT is a profoundly processive recombinant polymerase possessing outstanding strand displacement activity, which allows for highly efficient isothermal DNA amplification. The enzyme has a the capability to insert up to 70 thousands base per binding event. The polymerase likewise has a 3to 5 proofreading exonuclease activity acting especially on ssDNA or RNA. Hence, 3-modified primers are recommended.

Applications include:

Rolling Circle Amplification (RCA).

In situ genotyping with padlock probes.

Amplification of DNA for SNP and STR detection.

Unbiased amplification of whole genome.

DNA template preparation for sequencing.

RNA-primed DNA amplification.

Multiple displacement amplification (MDA).

Cell-free amplification of DNA from single cells.

Amplification of DNA from environmental samples.

Product Specifications:

Product name - phi29 Polymerase ,5000 U

Catalog No - EN20-050

Brand: BLIRT

Pack size: 5000 U

Product Features:

Recombinant polymerase derived from the Bacillus subtilis phage phi29 and over-expressed in E. coli.

Extremely processive polymerase, enabling amplification up to 70 kbp.

Extremely high yields of amplified DNA can be obtained even from minute amounts of template.

High-fidelity polymerase possesses a 3-5 exonuclease (proofreading) activity acting preferentially on ssDNA or RNA.

Isothermal polymerase no need for thermal cycling.

Due to heat lability of the enzyme, it is possible to use the amplification products directly in the downstream applications

Unit definition: One unit of phi29 DNA polymerase is defined as the amount of enzyme that will incorporate 0.5 pmol of dCMP into a polynucleotide fraction in 10 minutes at 30C under standard assay conditions.