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Plasmid DNA Isolation Kit- MPK1

Plasmid DNA Isolation Kit- MPK1

₹ 2,900.00 *
(0)
  • Brand: Aura Biotech
  • Catalog No.: ABT-171S
  • Quantity/Unit: 20 Rxns/Pack
  • Usually Shipped in: 4-7 Days

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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Plasmid DNA Isolation Kit is supplied by the company, Aura Biotech.

Plasmid DNA Isolation Kit was developed for the extraction of highly purified plasmid DNA from cultured bacterial cells within 20 min. The overall principle combines modified alkaline lysis method. Collected cells are resuspended in Resuspension Buffer. Following the addition of Lysis Buffer and Neutralization Buffer to the lysate, the chromosomal DNA and cell debris will be forming an insoluble aggregate. The insoluble protein aggregate is removed following centrifugation and transfer the clear lysate to the DNA binding filter tube. The cleared lysate contains a chaotropic salt originating from Neutralization Buffer which helps the binding of the plasmid DNA on the membrane surface. The DNA binding filter which is packed with silica-based membrane has enough surface area to bind up to 15-20 g of plasmid DNA. Any salts and precipitates are eliminated by addition of the Washing Buffer. Finally, highly purified plasmid DNA can be eluted with Elution Buffer or Nuclease free autoclaved distilled water (not provided).

Product specifications:

  • Catalog number: MPK1
  • Brand: Aura Biotech
  • Quantity/unit: Pack of 20rxns

Product features:

  • Efficienthigh yields of up to 15-20 g of high quality plasmid DNA

  • Fastprocedure takes less than 20 minutes

  • Convenientno phenol/chloroform extraction

  • Purepurified DNA is immediately ready to use

Applications

Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures

Fast Digest or conventional restriction digestion

Automated fluorescent and radioactive sequencing

PCR

In vitro transcription

Transformation, etc

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