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Quick Ligase  ,150 reactions

Quick Ligase ,150 reactions

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  • Brand: Blirt
  • Catalog No.: EN12-150
  • Quantity/Unit: 150 reactions/bottle

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Quick Ligase ,150 reactions supplied by BLIRT is an ATP-dependent recombinant enzyme isolated from Escherichia coli strain. The enzyme catalyzes the phosphodiester bond formation between juxtaposed 5-phosphate and 3-hydroxyl termini in duplex DNA or RNA. It joins both blunt-ended and cohesive-ended restriction fragments of DNA, as well as repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

Applications include:

Ligation in just 5 15 minutes

Cloning PCR products

Cloning restriction fragments

Joining double-stranded oligonucleotide linkers or adaptors to DNA

Site-directed mutagenesis

Nick repair in duplex DNA, RNA or DNA/RNA hybrids

Self-circularization of linear DNA

LM PCR methods (Ligation Mediated PCR), e.g. amplified fragment length polymorphism (AFLP)

Product Specifications:

Product name - Quick Ligase ,150 reactions

Catalog No - EN12-150

Brand: BLIRT

Pack size: 150 reactions

Product Features:

Storage temperature: 20C

Unit definition: One (Weiss) unit of Quick Ligase catalyzes the conversion of 1 nmol of 32P from pyrophosphate into Norit-adsorbable material in 20 minutes at 37C.