Quick Ligase ,150 reactions supplied by BLIRT is an ATP-dependent recombinant enzyme isolated from Escherichia coli strain. The enzyme catalyzes the phosphodiester bond formation between juxtaposed 5-phosphate and 3-hydroxyl termini in duplex DNA or RNA. It joins both blunt-ended and cohesive-ended restriction fragments of DNA, as well as repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
Applications include:
Ligation in just 5 15 minutes
Cloning PCR products
Cloning restriction fragments
Joining double-stranded oligonucleotide linkers or adaptors to DNA
Site-directed mutagenesis
Nick repair in duplex DNA, RNA or DNA/RNA hybrids
Self-circularization of linear DNA
LM PCR methods (Ligation Mediated PCR), e.g. amplified fragment length polymorphism (AFLP)
Product Specifications:
Product name - Quick Ligase ,150 reactions
Catalog No - EN12-150
Brand: BLIRT
Pack size: 150 reactions
Product Features:
Storage temperature: 20C
Unit definition: One (Weiss) unit of Quick Ligase catalyzes the conversion of 1 nmol of 32P from pyrophosphate into Norit-adsorbable material in 20 minutes at 37C.