RIBOPROTECT Hu RNase Inhibitor Lyo-ready New!,bulk supplied by BLIRT is a recombinant human placental protein expressed in Escherichia coli, in a special formulation (without glycerol). It has a molecular weight of 50 kDa and is known to have properties that helps inhibiting ribonuclease (RNase) activity of common eukaryotic enzymes like RNase A, B, and C via non-covalent binding in a 1:1 ratio. RIBOPROTECT Hu is employed where RNA quality and experiment results might get degraded due to the presence of RNases. It is utilized in in RNA isolation, cDNA synthesis, RT-PCR, RT-qPCR, RT-LAMP, in vitro transcription and translation, or RNase-free monoclonal antibody preparation. The enzyme does not have affect RNase 1, RNase T1, RNase T2, S1 nuclease, and RNase H and is compatible with DNA Polymerases and AMV or M-MuLV Reverse Transcriptases. Formulation of RIBOPROTECT Hu RNase Inhibitor Lyo-Ready enables its usage directly in the lyophilization process.

Applications include:

cDNA synthesis, RT-PCR, RT-qPCR, RT-LAMP

Optimal RNA protection in lyophilized diagnostic kits

RNA isolation and purification

in vitro transcription and translation

RNase-free monoclonal antibody preparation

Product Specifications:

Product name - RIBOPROTECT Hu RNase Inhibitor Lyo-ready New!,bulk

Catalog No - RT35L-B

Brand: BLIRT

Pack size: bulk

Product Features:

Formulation without glycerol for efficient freeze-drying

High functionality after lyophilization and reconstitution

Stable at 37C for at least 4 weeks with no loss of activity

Fully stable for at least 6 hours in 50C

Completely inhibits RNase A, RNase B, and RNaseC activity

Possibility of single-reaction or in-bulk lyophilization process

No influence on the polymerase or reverse transcriptase activity

Free of DNase and RNase activity

Active in diverse reaction conditions and in various buffers

Active over a broad range of pH and DTT

High concentration formulation available (100 kU/ml)