RPMI-1640 w/ L-Glutamine and 25mM HEPES Buffer w/o Sodium bicarbonate-AT060-20L

RPMI-1640 w/ L-Glutamine and 25mM HEPES Buffer w/o Sodium bicarbonate-AT060-20L

₹ 7,500.00 *


Catalog No.: AT060-20L

Quantity/Unit: 20lt/EA

Usually Shipped in: 2-3 Weeks

Min Orderable Qty : 1 Pack

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For lab/research use only, unless otherwise specified

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Product Description

RPMI 1640 With L- Glutamine and 25mM HEPES buffer Without Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 6.4 -7.0 whereas the pH with Sodium Bicarbonate is between 6.7 -7.3. The osmolality without Sodium Bicarbonate is in the range of 280.00 -320.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 320.00 -360.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Calcium nitrate tetrahydrate (100.00)

• Magnesium sulphate anhydrous (48.84)

• Potassium chloride (400.00)

• Sodium chloride (6000.00)

• Sodium phosphate dibasic anhydrous (800.00)

• Amino acids

• Choline chloride (3.00)

• D-Biotin (0.20)

• D-Ca-Pantothenate (0.25)

• Folic acid (1.00)

• Niacinamide (1.00)

• Pyridoxine hydrochloride (1.00)

• Riboflavin (0.20)

• Thiamine hydrochloride (1.00)

• Vitamin B12 (0.005)

• i-Inositol (35.00)

• p-Amino benzoic acid (PABA) (1.00)

• D-Glucose (2000.00)

• Glutathione reduced (1.00)

• HEPES Buffer (5958.00)

• Phenol Red Sodium Salt (5.30)

Roswell Park Memorial Institute (RPMI) media are a series of media developed by Moore et al for the culture of human normal and neoplastic cells in vitro. RPMI 1640 is the most commonly used medium in the series. A modification of McCoy's 5A medium, the medium was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Presently the medium is extensively used for a wide range of anchorage dependant cell lines. The medium needs to be supplemented with 5-20

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