RPMI-1640 With L-Glutamine Without Glucose and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 7.2 -7.8 whereas the pH with Sodium Bicarbonate is between 7.4 -8.0. The osmolality without Sodium Bicarbonate is in the range of 230.00 -270.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 280.00 -320.00 Osm/Kg H20. It is advisable to store this product between 2-8C. The media composition (in mg/L) is as follows:

Calcium nitrate tetrahydrate (100.00)

Glutathione reduced (1.00)

Magnesium sulphate anhydrous (48.84)

Phenol red sodium salt (5.30)

Potassium chloride (400.00)

Sodium chloride (6000.00)

Sodium phosphate dibasic anhydrous (800.00)

Amino acids

Choline chloride (3.00)

D-Biotin (0.20)

D-Ca-Pantothenate (0.25)

Folic acid (1.00)

Niacinamide (1.00)

Pyridoxine hydrochloride (1.00)

Riboflavin (0.20)

Thiamine hydrochloride (1.00)

Vitamin B12 (0.005)

i-Inositol (35.00)

p-Amino benzoic acid (PABA) (1.00)

Roswell Park Memorial Institute (RPMI) media are a series of media developed by Moore et al for the culture of human normal and neoplastic cells in vitro. RPMI 1640 is the most commonly used medium in the series. A modification of McCoy's 5A medium, the medium was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Presently the medium is extensively used for a wide range of anchorage dependant cell lines. The medium needs to be supplemented with 5-20