RPMI-1640 w/ L-Glutamine w/o Sodium bicarbonate-AT028-20L

Roswell Park Memorial Institute (RPMI) media with L-glutamine and without sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 7.2 -7.8 whereas the pH with Sodium Bicarbonate is between 7.6 -8.2. The osmolality without Sodium Bicarbonate is in the range of 230.00 -270.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 280.00 -320.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Calcium nitrate tetrahydrate (100.00) 

• Magnesium sulphate anhydrous (48.84) 

• Potassium chloride (400.00)  

• Sodium chloride (6000.00)

• Sodium phosphate dibasic anhydrous (800.00)

• Amino acids

• Choline chloride (3.00) 

• D-Biotin (0.20) 

• D-Ca-Pantothenate (0.25) 

• Folic acid (1.00)  

• Niacinamide (1.00) 

• Pyridoxine hydrochloride (1.00)

• Riboflavin (0.20)