Roswell Park Memorial Institute (RPMI) media with L-glutamine and without sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 7.2 -7.8 whereas the pH with Sodium Bicarbonate is between 7.6 -8.2. The osmolality without Sodium Bicarbonate is in the range of 230.00 -270.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 280.00 -320.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium nitrate tetrahydrate (100.00)

Magnesium sulphate anhydrous (48.84)

Potassium chloride (400.00)

Sodium chloride (6000.00)

Sodium phosphate dibasic anhydrous (800.00)

Amino acids

Choline chloride (3.00)

D-Biotin (0.20)

D-Ca-Pantothenate (0.25)

Folic acid (1.00)

Niacinamide (1.00)

Pyridoxine hydrochloride (1.00)

Riboflavin (0.20)