RPMI-1640 w/o L-Glutamine and Sodium bicarbonate-AT028A-1L

RPMI-1640 w/o L-Glutamine and Sodium bicarbonate-AT028A-1L

₹ 440.00 *

Brand: HIMEDIA

Catalog No.: AT028A-1L

Quantity/Unit: 1lt/EA

Usually Shipped in: 2-3 Weeks

Qty:
Min Orderable Qty : 1 Pack

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For lab/research use only, unless otherwise specified

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Product Description

Roswell Park Memorial Institute (RPMI) media without L-glutamine and sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 7.2 -7.8 whereas the pH with Sodium Bicarbonate is between 7.6 -8.2. The osmolality without Sodium Bicarbonate is in the range of 230.00 -270.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 280.00 -320.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Calcium nitrate tetrahydrate (100.00) 

• Magnesium sulphate anhydrous (48.84) 

• Potassium chloride (400.00)  

• Sodium chloride (6000.00)

• Sodium phosphate dibasic anhydrous (800.00)

• Amino acids

• Choline chloride (3.00) 

• D-Biotin (0.20) 

• D-Ca-Pantothenate (0.25) 

• Folic acid (1.00)  

• Niacinamide (1.00) 

• Pyridoxine hydrochloride (1.00)

• Riboflavin (0.20)

• Thiamine hydrochloride (1.00) 

• Vitamin B12 (0.005) 

• i-Inositol (35.00)

• p-Amino benzoic acid (PABA) (1.00)

• D-Glucose (2000.00)

• Glutathione reduced (1.00) 

• Phenol red sodium salt (5.30)

Roswell Park Memorial Institute (RPMI) media are a series of media developed by Moore et al for the culture of human normal and neoplastic cells in vitro. RPMI 1640 is the most commonly used medium in the series. It is a modification of McCoy's 5A medium which was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. This medium is extensively used for a wide range of anchorage dependant cell lines. RPMI media needs to be supplemented with 5-20

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