SALTOnase (HL-Nuclease),5000 U supplied by BLIRT is a recombinant endonuclease produced in E.coli and is originated from a psychrophilic bacteria which efficiently digests all types of DNA and RNA substrates in different buffer conditions and a wide range of temperatures. It is a cold-active, heat-labile recombinant endonuclease that is extremely active in demanding conditions like low temperatures and environment with high salt content. It is therefore utilized to remove undesired nucleic acids contamination during purification of proteins in laboratory and manufacturing workflows.

Applications include:

Purification of biologics from residual nucleic acids in biopharma manufacturing.

Purification of recombinant proteins and enzymes for research and diagnostic use.

Removal of undesired nucleic acids contamination in molecular biology reagents in demanding systems.

Reduction of viscosity in biological samples (during production, automation)

Product Specifications:

Product name - SALTOnase (HL-Nuclease),5000 U

Catalog No - EN32-050

Brand: BLIRT

Pack size: 5000 U

Product Features:

Highly active in a broad range of temperatures (>20% at 8 45C).

Extreme nucleolytic activity at high salt concentrations (optimal concentration range for NaCl or KCl is 0 1.1 M), and other buffer additives, which can significantly improve efficiency and purification yield in various workflows.

Highly active in the typical buffers and grow media.

Requires 1 mM Mg2+ to activate and shows a broad spectrum of pH activity (optimum at pH 7.5 9.0).

Irreversible thermal inactivation at low temperature (15 min at 52C in the presence of 1 mM DTT)

Unit definition: One unit (U) is defined as the amount of enzyme that causes an increase in absorbance at 260 nm of 1.0 in 30 minutes at 37C in 50 mM Tris-HCl buffer, pH 8.0 (25C) supplemented with 5 mM MgCl2, 0.1 mg/ml BSA and 0.5 mg/ml herring sperm DNA as a substrate.