Synthetic defined yeast media includes all necessary components for selective growth of Saccharomyces cerevisiae. MP Biomedicals offers synthetic defined broth (SD) and synthetic defined agar (SDA) in a variety of formats for flexibility and convenience. Recommended Use: Add contents of the pouch (13.7 g) to 500 mL or (27.4 g) to 1000 mL of distilled, deionized water. Add agar if pouring plates. Autoclave at 121 C for 15 minutes for complete synthetic defined single drop-out medium for the growth of S.cerevisiae.