Sheep Blood Agar Modified Plate is supplied by HiMedia. It contains 25ml of sterile ready prepared media in 90mm disposable plates. This light amber coloured medium has a pH in the range of 7.1 to 7.5. Sheep Blood Agar Base is generally used for the cultivation and studying haemolytic reactions of Bacillus cereus in accordance with ISO 21871:2006. Composition (in gm/L) is as follows:

Enzymatic digest of casein (15.00)

Enzymatic digest of soya (5.00)

Sodium chloride (5.00)

Agar (12.50)

Bacillus cereus is a gram-positive aerobic or facultatively anaerobic, motile, spore forming, rod shaped bacterium which is widely distributed environmentally. It is associated mainly with food poisoning as it has been reported to be cause of serious and fatal non- gastointestinal-tract infections. Sheep Blood Agar Base, Modified with added sheep blood was developed to allow maximum recovery of B.cereus without interfering with their haemolytic reactions. This medium is formulated in accordance with ISO. It was formulated to be compatible with sheep blood and enable improved haemolytic reactions of organisms. Enzymatic digest of casein and soya peptone provide nitrogen, carbon, amino acids and vitamins. Sodium chloride is used to maintain the osmotic balance.