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Sheep Blood Agar Plate-MP1301-20PT

Sheep Blood Agar Plate-MP1301-20PT

₹ 1,605.00 *
(0)
  • Brand: HIMEDIA
  • Catalog No.: MP1301-20PT
  • Quantity/Unit: 20plts/Pack
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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Sheep Blood Agar Plate is supplied by HiMedia. It contains 25ml of sterile ready prepared media in 90mm disposable plates. This red coloured medium has a pH in the range of 7.1 to 7.5. Sheep Blood Agar is generally used for the cultivation of fastidious organisms and studying haemolytic reactions. It provides improved and enhanced haemolysis. It is advisable to store this product between 2-8C. Composition (in gm/L) is as follows:

Casein enzymic hydrolysate (14.00)

Peptic digest of animal tissue (4.50)

Yeast extract (4.50)

Sodium chloride (5.00)

Agar (12.50)

Sheep Blood (5.00)

Haemolysins are basically exotoxins produced by bacteria that lyse red blood cells. This haemolytic reaction can be visualized on blood agar plates.If the colonies of haemolytic bacteria may be seen surrounded by a clear, colourless zone where the red blood cells have been lysed and the haemoglobin destroyed to a colourless compound. This is called beta haemolysis. Other types of bacteria can reduce haemoglobin to methaemoglobin which produces a greenish zone around the colonies and this is called alpha haemolysis. Gamma haemolysis means no haemolysis i.e., no change in the medium is observed. Blood Agar Base No. 2, supplemented with sheep blood was used to study haemolytic reactions (patterns) of organisms. But this gave mixed haemolytic (a and b) reactions due to the physiological differences between sheep blood and horse blood. Sheep Blood Agar Base with added sheep blood was then developed to allow maximum recovery of organisms without interfering with their haemolytic reactions. Sheep Blood Agar Base is compatible with sheep blood and gives improved haemolytic reactions of organisms. Casein enzymic hydrolysate and yeast extract present in the medium provide nitrogen, carbon, amino acids and vitamins. Peptic digest of animal tissue acts as the nitrogen source. Sodium chloride maintains the osmotic balance. Sheep Blood Agar Base showed considerable improvement and the expected beta haemolytic reactions with S. pyogenes in comparison to other blood agar bases supplemented with blood.

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