Stoffel Fragment DNA Polymerase, 1000 U supplied by BLIRT is a recombinant, fragment of thermostable Taq DNA polymerase isolated from Thermus aquaticus that has a molecular weight of 62.7 kDa. It is employed in various applications requiring DNA synthesis in extremely high temperatures. Stoffel fragment is encoded by a modified form of the Thermus aquaticus DNA polymerase gene which has been inserted into an Escherichia coli host. The modified gene encodes a 540 amino acid fragment lacking the N-terminal 292 amino acid portion of the full length TaqNova DNA Polymerase. It is known to be twice as thermostable as TaqNova DNA Polymerase and demands greater concentration levels of MgCl2 and lower ionic strength for its optimum enzymatic activity.

Various applications include:

Efficient amplification of short and medium size sequences.

Diagnostic PCR.

Strongly suggested for GC rich and secondary structure templates the increase thermal stability of the TaqNova Stoffel may lead to superior amplification of excessively GC rich templates and templates with secondary structure by allowing the use of denaturation temperatures as high as 98C.

Multiplex PCR no need for MgCl2 optimization the vast magnesium optimum for TaqNova Stoffel DNA Polymerase reduces the need for magnesium optimization experiments and increases the easiness of Multiplex PCR optimization, the simultaneous amplification of multi targets in the same reaction.

Genotyping TaqNova Stoffel DNA Polymerase shows great performance in genetic mapping using primers with arbitrary sequences (RAPD).

ASA PCR (Allele Specific Amplification PCR) amplification depends of 3 terminal bases, that complement the primer.

Product Specifications:

Product name - Stoffel Fragment DNA Polymerase, 1000 U

Catalog No - RP810

Brand: BLIRT

Pack size: 1000 U

Product Features:

Consistent results

Suitable for a wide range of applications

High-purity recombinant enzyme confirmed 95% purity recombinant enzyme

High-efficiency enzyme extreme yields

Easy to use no optimization required

Maximum performance with improved reaction buffer formulation

No exonuclease activity

High thermostability the half-life of the enzyme is 20 minutes at 97.5C

Amplifies fragments of up to 5 kb

Leaves A overhangs

Unit definition: One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 72C in a 50 l reaction..