Specific antibodies are used commonly for the identification and characterization of proteins during techniques such as ELISA, immune-electrophoresis, western blotting etc. These antibodies can be monoclonal or polyclonal, based on the requirement of the assay. The antibodies can also be conjugated to several labels, which can be used to detect the antibodies bound to specific target antigens for quantitative and qualitative analysis. One such label is biotin which has a very strong interaction with a bacterial protein known as streptavidin. To identify the binding of biotin labelled antibodies to a specific antigen, streptavidin conjugated with horse radish peroxidase is used. This enzyme uses hydrogen peroxidase to catalyse the oxidation of several substances, such as TMB. The resulting coloured product can be quantified using spectrometric methods. The Streptavidin-HRP conjugate is provided by Genei for use in assays such as ELISA, western blot, etc for detection of biotin labelled antibodies.

Product specification:

Product name: Streptavidin HRP conjugate

Quantity: 0.5 mL

Brand: Genei

Catalogue number:1140975001730

Storage: -20C