Stripping Buffer is supplied by HiMedia. Stripping Buffer is a ready-to-use reagent specifically formulated for efficient stripping of primary and fluorescent labelled secondary antibodies probed during Western blotting procedure. Stripping Buffer is gentle and effective on both nitrocellulose and PVDF Western blot membranes. It is advisable to store this product at 15-25C.

In Western blotting procedure Stripping Buffer is used for breaking antibody-antigen interactions to remove primary and secondary antibodies from the nitrocellulose and PVDF membranes which subsequently can be re-probed several times using different antibodies. When more than one target has to be probed then the same membrane can be stripped and re-probed instead of running and blotting multiple gels which has the advantage of saving samples, reagents and time. Blots can be stripped multiple times for the repeated use of membranes. PVDF and nitrocellulose membranes probed by Western blotting procedures and detected by chemiluminescent or other non-precipitating substrates can be stripped and re-probed using this buffer.