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T4 DNA Ligase-1100100021730

T4 DNA Ligase-1100100021730

₹ 2,780.00 *
(0)
  • Brand: Bangalore GeNei
  • Catalog No.: 1100100021730
  • Quantity/Unit: 8000 Units/Vial
  • Availability: In stock

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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T4 DNA is ligase is provided by Genei to suit needs of molecular biology. Ligases are a class of enzymes which act as a glue for DNA fragments. The enzyme joins strands of DNA together by catalysing the creating phosphodiester bonds at 3phosphate and 5 hydroxy terminal. DNA ligase plays an important role in DNA damage repair mechanisms and in DNA replication. T4 DNA ligase is obtained from the bacteriophage T4. It can catalyse the joining of both blunt ends and sticky ends in DNA fragments. The enzyme aids in the joining of DNA fragments, oligonucleotides and it allows the formation of RNA-DNA hybrids. But the enzyme has no activity on single stranded DNA fragments. The enzyme requires ATP as an important co-factor to carry out the ligase activity.

T4 DNA ligase from Genei is provided as a vial of 40,000 units, where one unit of cohesive end ligase can be described as the amount of ligase which is required for the partial (50%) ligation of DNA digested with either Lambda/Hind III in a time frame of 30 mintues. The reaction conditions for the same are temperature 16 C, 20L reaction mix and 0.12M concentration of 5 DNA termini. One ligase unit can also be defined as the equivalent of 0.015 ATP-PP exchange units.

Genei ensures the quality of the T4 DNA ligase by using the enzyme for their in-house quality checks for their restriction enzymes and by using it during Blue-white standard assay. Its activity is also checked in cloning techniques. In one such experiments, the blunt end ligation activity of 1 L of the enzyme is checked against HaeIII blunt end fragments (at 22C and at different time frames).

Product name: T4 DNA Ligase

Brand: Genei

Catalogue number: 1100100021730

Amount: 4000 units; 400 U/L

Price: 5,700.00

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