MBT091-10000U is referred as T4 DNA Ligase, under HiMedia catalogue. MBT091T4 DNA Ligase is provided with 10X Ligase Buffer.
Specifications:
T4 DNA Ligase seals single stranded nicks in duplex DNA, RNA or DNA-RNA hybrids
ATP is an essential cofactor for the reaction
Concentration: 200 units/l
T4 DNA Ligase enzyme should be stored at -20C
Thermal inactivation: 65C for 15 minutes
Applications:
1. Catalyses the linkage of 5 or 3 blunt/cohesive ends of double-stranded DNA by
formation of phosphodiester bond.
2. Joining of oligonucleotide linkers or adapters to blunt ends.
3. Repair nicks formation in duplex nucleic acids.
Pack Size 10000 units