T4 DNA Ligase,2500 U supplied by BLIRT is an ATP-dependent recombinant enzyme that derives from Escherichia coli strain. It catalyzes phosphodiester bond formation between juxtaposed 5-phosphate and 3-hydroxyl termini in duplex DNA or RNA. It joins blunt-ended and cohesive-ended restriction fragments of DNA along with repairing the single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

Applications include:

Molecular cloning of PCR products or restriction fragments.

Joining double-stranded oligonucleotide linkers or adaptors to DNA.

Site-directed mutagenesis.

Nick repair in duplex DNA, RNA or DNA/RNA hybrids.

Self-circularization of linear DNA.

LM PCR methods (Ligation Mediated PCR).

Product Specifications:

Product name - T4 DNA Ligase,2500 U

Catalog No - EN11-250

Brand: BLIRT

Pack size: 2500 U

Product Features:

ATP-dependent recombinant DNA ligases over-expressed in E. coli.

Available also in special formulation for very fast and efficient ligation of DNA fragments with compatible cohesive or blunt ends in 5 and 15 minutes respectively

Unit definition -One (Weiss) unit of T4 DNA Ligase catalyzes the conversion of 1 nmol of 32P from pyrophosphate into Norit-adsorbable material in 30 minutes at 37C. -One Weiss unit is equivalent an approximately 200 cohesive end units.