T4 Polynucleotide Kinase-1100400051730
- Brand: Bangalore GeNei
- Catalog No.: 1100400051730
- Quantity/Unit: 1000 Units/Vial
- Availability: In stock
Min Orderable Qty : 1 Pack
For lab/research use only, unless otherwise specified
T4 Polynucleotide kinase is provided by Genei for molecular biology applications. Kinases are enzymes which add phosphate groups to a substrate. The phosphate group is derived from a high energy molecule such as ATP. T4 Polynucleotide kinase is involved in the transfer for phosphate group from ATP to the 5' hydroxyl end of DNA or RNA. This kinase also has the property of removal of 3' phosphate groups from DNA or RNA. It is usually isolated from a recombinant strain of E.coli contains the gene coding for T4 Polynucleotide kinase. The enzyme finds application in probe end labelling and DNA sequencing, it aids in ligation reaction by creating a 5' phosphate group and by removing a 3' phosphate group. GeNei provides 1000 units of T4 Polynucleotide kinase, where one unit is described as the quantity of the enzyme required for catalyzing the formation of one nanomole of acid insoluble 32P (Reaction conditions - 37C, 30 minutes). To ensure the quality of the product, several labelling and kinetics studies were performed.
The assay buffer (10 X) provided contains the following :
70 mM Tris HCl (pH 7.6)
10 mM Magnesium chloride
5 mM DTT
The storage buffer has the following components :
10 mM Tris HCl (pH 7.4)
0.1 mM EDTA
50 mM Potassium chloride
1 mM DTT
Nuclease-free BSA 200 ug/mL
50% glycerol
Product specifications :
Product name : T4 Polynucleotide Kinase, 1000Units
Quantity : 1000 units, 10 units/uL
Brand : Genei
Catalogue number :1100400051730
Price: 5,870.00
Storage : - 20C