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Taq DNA Polymerase from Thermus aquaticus without MgCl2

Taq DNA Polymerase from Thermus aquaticus without MgCl2

₹ 13,775.10 *
(0)
  • Brand: Sigma-Aldrich
  • Catalog No.: D4545-1.5KU
  • CAS No: 9012-90-2
  • Quantity/Unit: 1500 units/Pack
  • Usually Shipped in: 4-7 Days

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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Taq DNA Polymerase from Thermus aquaticus without MgCl2

Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacteriumThermus aquaticus. A stable deoxyribonucleic acid (DNA) polymerase (with a temperature optimum of 80C) has been purified from the extreme thermophileThermus aquaticus. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities. Maximal activity of the enzyme needs all four deoxyribonucleotides and activated calf thymus DNA.

Application:

Taq DNA Polymerase from Thermus aquaticus is a thermostable DNA polymerase that is used for the DNA polymerase chain reaction (PCR) in order to amplify DNA sequences.

Taq DNA Polymerase from Thermus aquaticus has been used in the process of DNA extraction (during gene amplification and sequencing). It has been used in genotyping.[1] It has also been used in polymerase chain reaction (PCR) to study the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8).

Features and Benefits

  • MgCl2 provided in a separate tube to allow MgCl2 optimization
  • Can withstand repeated heating to 95 C without significant loss of activity

Packaging

Taq DNA Polymerase with 10 reaction buffer without MgCl2. Includes a separate tube of 25 mM MgCl2