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UDGase,2500 U

UDGase,2500 U

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  • Brand: Blirt
  • Catalog No.: EN19-250
  • Quantity/Unit: 2500 U/bottle

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UDGase,2500 U supplied by BLIRT is an enzyme that is derived from E. coli. Uracil DNA Glycosylase (UDG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single-stranded or double-stranded DNA. The enzyme shows no activity on RNA or oligonucleotides.

Applications include:

Widely used to eliminate carry-over contamination in PCR.

As a probe for protein-DNA interaction studies.

Glycosylase mediated single nucleotide polymorphism detection (GMPD).

Molecular cloning of PCR products.

Product Specifications:

Product name - UDGase,2500 U

Catalog No - EN19-250

Brand: BLIRT

Pack size: 2500 U

Product Features:

Modified recombinant E. coli enzyme over-expressed in E. coli.

Sensitive mutant is irreversibly inactive after heating.

Catalyses the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides.

Active over a broad pH range (optimum at pH 8.0)

Unit definition: One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from uracil-containing dsDNA. Activity is measured by release of [3H]-uracil in a 50 l reaction containing 0.2 g DNA in 30 minutes at 37C