UDGase,2500 U supplied by BLIRT is an enzyme that is derived from E. coli. Uracil DNA Glycosylase (UDG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single-stranded or double-stranded DNA. The enzyme shows no activity on RNA or oligonucleotides.
Applications include:
Widely used to eliminate carry-over contamination in PCR.
As a probe for protein-DNA interaction studies.
Glycosylase mediated single nucleotide polymorphism detection (GMPD).
Molecular cloning of PCR products.
Product Specifications:
Product name - UDGase,2500 U
Catalog No - EN19-250
Brand: BLIRT
Pack size: 2500 U
Product Features:
Modified recombinant E. coli enzyme over-expressed in E. coli.
Sensitive mutant is irreversibly inactive after heating.
Catalyses the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides.
Active over a broad pH range (optimum at pH 8.0)
Unit definition: One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from uracil-containing dsDNA. Activity is measured by release of [3H]-uracil in a 50 l reaction containing 0.2 g DNA in 30 minutes at 37C