Violet Red Bile Agar
Catalog No.: DM286-100g
Usually Shipped in: 1-2 Weeks
For lab/research use only, unless otherwise specified
- Product Details
High grade Violet Red Bile Agar is supplied by Micromaster. It is available in the form of a light yellow to light pink homogenous free flowing powder which has a pH in the range of 7.2 to 7.6. It is advisable to store this product below 30⁰C.
- Quantity provided: 100 g/pack
Violet Red Bile Agar (VRBA) is a selective medium used for the isolation, detection and enumeration of coli-aerogenes bacteria in water, milk, other dairy food products and also from clinical samples. The coliform group consists of several genera of bacteria belonging to the family Enterobacteriaceae. Violet Red Bile Agar, a modification of MacConkeys original formulation is used for the enumeration of coli-aerogenes bacterial group. It relies on the use of the selective inhibitory components crystals violet and bile salts and the indicator system lactose, and neutral red. Thus, the growth of many unwanted organisms is suppressed, while tentative identification of sought bacteria can be made. Organisms, which rapidly attack lactose, produce purple colonies surrounded by purple halos. Peptic digest of animal tissue and yeast extract serve as sources of carbon, nitrogen, vitamins and other essential growth nutrients. Lactose is the fermentable carbohydrate, utilization of which leads to the production of acids. Neutral red indicator detects the acidity so formed. Crystal violet and bile salts mixture help to inhibit the accompanying gram-positive and unrelated flora. Sodium chloride maintains the osmotic equilibrium. Violet Red Bile Agar is not completely specific for enteric; other accompanying bacteria may give the same reaction. Further biochemical tests are necessary for positive identification.