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ZymeFree Enzyme Free Cell Dissociation Reagent in Dulbeccos Phosphate Buffered Saline 1X-TCL028-5X100ML

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ZymeFree Enzyme Free Cell Dissociation Reagent in Dulbecco's Phosphate Buffered Saline is supplied by HiMedia. It is available in the form of a clear colourless solution with a pH in the range of 5.8 to 6.4 and an osmolality between 280.00 -320.00 mOsm/Kg H2O. It is advisable to store this product between 15-30C.

ZymeFree cell dissociation reagent in Dulbecco's Phosphate buffered saline is used for gentle removal of adherent cells from each other and from the surface of a culture vessel. This enzyme free formulation helps in preserving the structural and functional integrity of the cell surface proteins. It also helps in avoiding the cytotoxic effects sometimes associated with the chelating agents. This product is very useful for studies that require intact cell surface proteins such as ligand binding, flow cytometry and immunohistochemistry and as a trypsin alternative in serum free culture work. ZymeFree works as quick as trypsin on most cell lines. It holds the advantage over enzyme based dissociation solutions as it does not cause cytotoxicity, even after cells are exposed to it for prolonged period.