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Cloning Buffers and Reagents

Cloning is the process of creating a recombinant DNA, packing it within a host system and propagating the recombinant DNA within the host. Molecular cloning uses a number of reagents and buffers. Generally, the recombinant DNA is incorporated into a plasmid. For the isolation of plasmid, several buffers are used such as cell lysis buffers, RNases, resuspension solutions etc. In case of plasmid isolation using columns, wash buffers, elution buffers etc. must be used. Further, once the plasmid is isolated, the target DNA and the plasmid must be cut with the same restriction enzymes and ligated together. All these processes require specific buffers to maintain the optimum experimental conditions. Then the recombinant plasmid is inserted into a host. To confirm recombination into the host, several techniques such as plating on IPTG X-gal plates are used. This is a simplified process to select recombinants and screen-out the non-recombinants.

Biomall provides customers with several buffers and reagent required during cloning. Elution buffers, wash buffers, IPTG, X-gal, lysis buffers and several other reagents are available.

PRODUCTS

X-Gal, 1 gram

X-Gal, 1 gram

Brand: Fermentas

Catalog No.: R0404

Quantity/Unit: 1 gram/Pack

Usually Shipped in: 1-2 Weeks

X-Gal Solution  ready-to-use, 10 mL

X-Gal Solution ready-to-use, 10 mL

Brand: Fermentas

Catalog No.: R0941

Quantity/Unit: 10 mL/Pack

Usually Shipped in: 1-2 Weeks

IPTG Solution  ready-to-use, 10 x 1.5 mL

IPTG Solution ready-to-use, 10 x 1.5 mL

₹ 7,305.00

Brand: Fermentas

Catalog No.: R1171

Quantity/Unit: 10 x 1.5 mL/Pack

Usually Shipped in: 1-2 Weeks

Resuspension Solution, 70 mL

Resuspension Solution, 70 mL

₹ 4,428.00

Brand: Fermentas

Catalog No.: R1213

Quantity/Unit: 70 mL/Bottle

Usually Shipped in: 1-2 Weeks

Lysis Solution , 70 mL

Lysis Solution , 70 mL

₹ 4,841.00

Brand: Fermentas

Catalog No.: R1223

Quantity/Unit: 70 mL/Bottle

Usually Shipped in: 1-2 Weeks

Neutralization Solution, 100 mL

Neutralization Solution, 100 mL

₹ 4,428.00

Brand: Fermentas

Catalog No.: R1233

Quantity/Unit: 100 mL/Bottle

Usually Shipped in: 1-2 Weeks

Wash Solution , 100 mL

Wash Solution , 100 mL

₹ 5,006.00

Brand: Fermentas

Catalog No.: R1243

Quantity/Unit: 100 mL/Bottle

Usually Shipped in: 1-2 Weeks

Elution Buffer, 30 mL for GeneJET Plasmid Miniprep Kit

Elution Buffer, 30 mL for GeneJET Plasmid Miniprep Kit

₹ 3,466.00

Brand: Fermentas

Catalog No.: R1263

Quantity/Unit: 30 mL/Bottle

Usually Shipped in: 1-2 Weeks

Resuspension Solution (for kits K0491/2)

Resuspension Solution (for kits K0491/2)

₹ 5,728.00

Brand: Fermentas

Catalog No.: R1581

Quantity/Unit: 240 mL/Bottle

Usually Shipped in: 1-2 Weeks

Resusp. Sol/GeneJET Endo-free

Resusp. Sol/GeneJET Endo-free

₹ 4,950.00

Brand: Fermentas

Catalog No.: R2221

Quantity/Unit: 84 mL/Bottle

Usually Shipped in: 1-2 Weeks

Lysis Sol/GeneJET Endo-free

Lysis Sol/GeneJET Endo-free

₹ 4,928.00

Brand: Fermentas

Catalog No.: R2231

Quantity/Unit: 84 mL/Bottle

Usually Shipped in: 1-2 Weeks

Neutral. Sol/GeneJET Endo-free

Neutral. Sol/GeneJET Endo-free

₹ 3,838.00

Brand: Fermentas

Catalog No.: R2241

Quantity/Unit: 84 mL/Bottle

Usually Shipped in: 1-2 Weeks

EBR/GeneJET Endo-free

EBR/GeneJET Endo-free

₹ 9,428.00

Brand: Fermentas

Catalog No.: R2251

Quantity/Unit: 12 mL/Bottle

Usually Shipped in: 1-2 Weeks

Wash Buf1/GeneJET Endo-free

Wash Buf1/GeneJET Endo-free

₹ 3,231.00

Brand: Fermentas

Catalog No.: R2261

Quantity/Unit: 75 mL/Bottle

Usually Shipped in: 1-2 Weeks

Wash Buf2/GeneJET Endo-free

Wash Buf2/GeneJET Endo-free

₹ 3,231.00

Brand: Fermentas

Catalog No.: R2271

Quantity/Unit: 75 mL/Bottle

Usually Shipped in: 1-2 Weeks