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Cloning Buffers and Reagents

Cloning is the process of creating a recombinant DNA, packing it within a host system and propagating the recombinant DNA within the host. Molecular cloning uses a number of reagents and buffers. Generally, the recombinant DNA is incorporated into a plasmid. For the isolation of plasmid, several buffers are used such as cell lysis buffers, RNases, resuspension solutions etc. In case of plasmid isolation using columns, wash buffers, elution buffers etc. must be used. Further, once the plasmid is isolated, the target DNA and the plasmid must be cut with the same restriction enzymes and ligated together. All these processes require specific buffers to maintain the optimum experimental conditions. Then the recombinant plasmid is inserted into a host. To confirm recombination into the host, several techniques such as plating on IPTG X-gal plates are used. This is a simplified process to select recombinants and screen-out the non-recombinants.

Biomall provides customers with several buffers and reagent required during cloning. Elution buffers, wash buffers, IPTG, X-gal, lysis buffers and several other reagents are available.

PRODUCTS

CHAPS, Molecular Biology Grade-3602000051730

CHAPS, Molecular Biology Grade-3602000051730

₹ 2,940.00

Brand: Bangalore GeNei

Catalog No.: 3602000051730

Quantity/Unit: 5 g/Pack

Usually Shipped in: 2-3 Weeks

EBR/GeneJET Endo-free

EBR/GeneJET Endo-free

₹ 8,343.46

Brand: Fermentas

Catalog No.: R2251

Quantity/Unit: 12 mL/Bottle

Usually Shipped in: 1-2 Weeks

Elution Buf/GeneJET Endo-free

Elution Buf/GeneJET Endo-free

₹ 2,859.15

Brand: Fermentas

Catalog No.: R2281

Quantity/Unit: 18 mL/Bottle

Usually Shipped in: 1-2 Weeks

Elution Buffer, 30 mL

Elution Buffer, 30 mL

₹ 3,067.05

Brand: Fermentas

Catalog No.: R1263

Quantity/Unit: 30 mL/Bottle

Usually Shipped in: 1-2 Weeks

HEPES Sodium Salt, Molecular Biology Grade-3601505001730

HEPES Sodium Salt, Molecular Biology Grade-3601505001730

₹ 13,460.00

Brand: Bangalore GeNei

Catalog No.: 3601505001730

Quantity/Unit: 500 g/Pack

Usually Shipped in: 2-3 Weeks

HEPES, Molecular Biology Grade-3601405001730

HEPES, Molecular Biology Grade-3601405001730

₹ 8,630.00

Brand: Bangalore GeNei

Catalog No.: 3601405001730

Quantity/Unit: 500 g/Pack

Usually Shipped in: 2-3 Weeks

IPTG dioxane-free, 1 gram

IPTG dioxane-free, 1 gram

Brand: Fermentas

Catalog No.: R0391

Quantity/Unit: 1 gram/Pack

Usually Shipped in: 1-2 Weeks

IPTG  dioxane-free, 5 gram

IPTG dioxane-free, 5 gram

₹ 9,740.71

Brand: Fermentas

Catalog No.: R0392

Quantity/Unit: 5 gram/Pack

Usually Shipped in: 1-2 Weeks

IPTG  dioxane-free, 5 x 5 gram

IPTG dioxane-free, 5 x 5 gram

₹ 32,790.05

Brand: Fermentas

Catalog No.: R0393

Quantity/Unit: 5 x 5 gram/Pack

Usually Shipped in: 1-2 Weeks

IPTG Solution  ready-to-use, 10 x 1.5 mL

IPTG Solution ready-to-use, 10 x 1.5 mL

₹ 6,464.28

Brand: Fermentas

Catalog No.: R1171

Quantity/Unit: 10 x 1.5 mL/Pack

Usually Shipped in: 1-2 Weeks

Lysis Sol/GeneJET Endo-free

Lysis Sol/GeneJET Endo-free

₹ 4,360.95

Brand: Fermentas

Catalog No.: R2231

Quantity/Unit: 84 mL/Bottle

Usually Shipped in: 1-2 Weeks

Lysis Solution , 70 mL

Lysis Solution , 70 mL

₹ 4,284.00

Brand: Fermentas

Catalog No.: R1223

Quantity/Unit: 70 mL/Bottle

Usually Shipped in: 1-2 Weeks

MOPS Sodium Salt, Molecular Biology Grade-3601905001730

MOPS Sodium Salt, Molecular Biology Grade-3601905001730

₹ 15,540.00

Brand: Bangalore GeNei

Catalog No.: 3601905001730

Quantity/Unit: 500 g/Pack

Usually Shipped in: 2-3 Weeks

MOPS, Molecular Biology Grade-3601805001730

MOPS, Molecular Biology Grade-3601805001730

₹ 10,080.00

Brand: Bangalore GeNei

Catalog No.: 3601805001730

Quantity/Unit: 500 g/Pack

Usually Shipped in: 2-3 Weeks

Neutral. Sol/GeneJET Endo-free

Neutral. Sol/GeneJET Endo-free

₹ 3,396.75

Brand: Fermentas

Catalog No.: R2241

Quantity/Unit: 84 mL/Bottle

Usually Shipped in: 1-2 Weeks