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Cloning Buffers and Reagents

Cloning Buffers and Reagents

Cloning is the process of creating a recombinant DNA, packing it within a host system and propagating the recombinant DNA within the host. Molecular cloning uses a number of reagents and buffers. Generally, the recombinant DNA is incorporated into a plasmid. For the isolation of plasmid, several buffers are used such as cell lysis buffers, RNases, resuspension solutions etc. In case of plasmid isolation using columns, wash buffers, elution buffers etc. must be used. Further, once the plasmid is isolated, the target DNA and the plasmid must be cut with the same restriction enzymes and ligated together. All these processes require specific buffers to maintain the optimum experimental conditions. Then the recombinant plasmid is inserted into a host. To confirm recombination into the host, several techniques such as plating on IPTG X-gal plates are used. This is a simplified process to select recombinants and screen-out the non-recombinants.

Biomall provides customers with several buffers and reagent required during cloning. Elution buffers, wash buffers, IPTG, X-gal, lysis buffers and several other reagents are available.

CHAPS, Molecular Biology Grade-3602000051730
₹ 3,729.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3602000051730
  • Quantity/Unit: 5 g/Pack
PIPES, Molecular Biology Grade-3601605001730
₹ 13,288.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3601605001730
  • Quantity/Unit: 500 g/Pack
Tris-HCl, Molecular Biology Grade-3601310001730
₹ 10,197.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3601310001730
  • Quantity/Unit: 1 Kg/Pack
X-Gal, 2 x 1 gram
₹ 0.00
  • Brand: Fermentas
  • Catalog No.: R0402
  • Quantity/Unit: 2 x 1 gram/Pack
  • Usually Shipped in: 1-2 Weeks
Tris-Base, Molecular Biology Grade-3600750001730
₹ 36,685.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3600750001730
  • Quantity/Unit: 5 Kg/Pack
MOPS Sodium Salt, Molecular Biology Grade-3601905001730
₹ 19,668.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3601905001730
  • Quantity/Unit: 500 g/Pack
Resuspension Solution, 70 mL
₹ 4,428.00
  • Brand: Fermentas
  • Catalog No.: R1213
  • Quantity/Unit: 70 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Lysis Solution , 70 mL
₹ 4,841.00
  • Brand: Fermentas
  • Catalog No.: R1223
  • Quantity/Unit: 70 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
MOPS, Molecular Biology Grade-3601805001730
₹ 12,760.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3601805001730
  • Quantity/Unit: 500 g/Pack
Elution Buf/GeneJET Endo-free
₹ 3,231.00
  • Brand: Fermentas
  • Catalog No.: R2281
  • Quantity/Unit: 18 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
IPTG dioxane-free, 5 gram
₹ 0.00
  • Brand: Fermentas
  • Catalog No.: R0392
  • Quantity/Unit: 5 gram/Pack
  • Usually Shipped in: 1-2 Weeks
IPTG Solution ready-to-use, 10 x 1.5 mL
₹ 7,305.00
  • Brand: Fermentas
  • Catalog No.: R1171
  • Quantity/Unit: 10 x 1.5 mL/Pack
  • Usually Shipped in: 1-2 Weeks
Wash Solution , 100 mL
₹ 5,006.00
  • Brand: Fermentas
  • Catalog No.: R1243
  • Quantity/Unit: 100 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
HEPES, Molecular Biology Grade-3601405001730
₹ 10,923.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3601405001730
  • Quantity/Unit: 500 g/Pack
HEPES Sodium Salt, Molecular Biology Grade-3601505001730
₹ 17,028.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3601505001730
  • Quantity/Unit: 500 g/Pack
X-Gal, 1 gram
₹ 0.00
  • Brand: Fermentas
  • Catalog No.: R0404
  • Quantity/Unit: 1 gram/Pack
  • Usually Shipped in: 1-2 Weeks
Wash Buf1/GeneJET Endo-free
₹ 3,231.00
  • Brand: Fermentas
  • Catalog No.: R2261
  • Quantity/Unit: 75 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Wash Buf2/GeneJET Endo-free
₹ 3,231.00
  • Brand: Fermentas
  • Catalog No.: R2271
  • Quantity/Unit: 75 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Tris-Base, Molecular Biology Grade-3600710001730
₹ 8,503.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3600710001730
  • Quantity/Unit: 1 Kg/Pack
Resusp. Sol/GeneJET Endo-free
₹ 4,950.00
  • Brand: Fermentas
  • Catalog No.: R2221
  • Quantity/Unit: 84 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Resuspension Solution (for kits K0491/2)
₹ 5,728.00
  • Brand: Fermentas
  • Catalog No.: R1581
  • Quantity/Unit: 240 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
EBR/GeneJET Endo-free
₹ 9,428.00
  • Brand: Fermentas
  • Catalog No.: R2251
  • Quantity/Unit: 12 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
IPTG dioxane-free, 1 gram
₹ 0.00
  • Brand: Fermentas
  • Catalog No.: R0391
  • Quantity/Unit: 1 gram/Pack
  • Usually Shipped in: 1-2 Weeks
IPTG dioxane-free, 5 x 5 gram
₹ 0.00
  • Brand: Fermentas
  • Catalog No.: R0393
  • Quantity/Unit: 5 x 5 gram/Pack
  • Usually Shipped in: 1-2 Weeks
Elution Buffer, 30 mL for GeneJET Plasmid Miniprep Kit
₹ 3,466.00
  • Brand: Fermentas
  • Catalog No.: R1263
  • Quantity/Unit: 30 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Neutralization Solution, 100 mL
₹ 4,428.00
  • Brand: Fermentas
  • Catalog No.: R1233
  • Quantity/Unit: 100 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Neutral. Sol/GeneJET Endo-free
₹ 3,838.00
  • Brand: Fermentas
  • Catalog No.: R2241
  • Quantity/Unit: 84 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
X-Gal, 0.5 gram
₹ 0.00
  • Brand: Fermentas
  • Catalog No.: R0401
  • Quantity/Unit: 0.5 gram/Pack
  • Usually Shipped in: 1-2 Weeks