DNA Electrophoresis
DNA electrophoresis is commonly performed using the technique called as Agarose gel electrophoresis - one of the most widely used technique in molecular biology to separate and visualize macromolecules like DNA on an agarose gel under the influence of an electric field. With a plastic comb, wells are dug into the gel. A standard gene ladder is loaded into the first well of the agarose followed by loading the samples in the rest. The gel is dipped into Tris buffer for electrical conduction. When an electric current is applied, negatively charged DNA migrates towards the positive electrode (Anode). A suitable dye is used to visualize the sample run. The separation occurs in the form of bands corresponding to DNA size and shape. An intercalating agent like Ethidium bromide which fluoresces in the presence of UV light is mixed with the sample to visualize DNA bands under A UV transilluminator.
This separation procedure requires a number of accessories, samples and apparatus. These include buffer solutions, DNA ladders, dyes, gels and solidifying agents, electrophoresis apparatus, casting trays, combs, power packs, electrodes, kits etc. Products required in DNA electrophoresis are available from popular brands such as HiMedia, Fisher BioReagents, Bangalore GeNei on Biomall.in.
Ethidium Bromide for Molecular Biology, 50mg
₹ 1,690.00
- Brand:
APS LABS - Catalog No.:
RC1079-1 - Quantity/Unit: 50mg/Vial
- Usually Shipped in: 4-7 Days
RNA Stabilizer Solution, 50ml
₹ 6,370.00
- Brand:
APS LABS - Catalog No.:
MAGSPIN-100 - Quantity/Unit: 50ml/Bottle
- Usually Shipped in: 4-7 Days
DNA Stabilizer Solution, 50 U
₹ 6,370.00
- Brand:
APS LABS - Catalog No.:
MAGSPIN-100A - Quantity/Unit: 50 U/Vial
- Usually Shipped in: 4-7 Days
TMB Liquid Substrate for Membranes, 100ml
₹ 3,900.00
- Brand:
APS LABS - Catalog No.:
APS-BL-54 - Quantity/Unit: 100ml/Bottle
- Usually Shipped in: 4-7 Days
Imidazole for Molecular Biology 99.5%, 25g
₹ 797.00
- Brand:
APS LABS - Catalog No.:
RC1106-1 - Quantity/Unit: 25g/Vial
- Usually Shipped in: 4-7 Days
Imidazole for Molecular Biology 99.5%, 100g
₹ 2,387.00
- Brand:
APS LABS - Catalog No.:
RC1106-2 - Quantity/Unit: 100g/Vial
- Usually Shipped in: 4-7 Days
Triton X-100 for Molecular Biology, 100g
₹ 1,144.00
- Brand:
APS LABS - Catalog No.:
RC35-1 - Quantity/Unit: 100g/Vial
- Usually Shipped in: 4-7 Days
Triton X-100 for Molecular Biology, 500g
₹ 5,096.00
- Brand:
APS LABS - Catalog No.:
RC35-2 - Quantity/Unit: 500g/Vial
- Usually Shipped in: 4-7 Days
50X TAE Buffer MAGSPIN-114A
₹ 7,800.00
- Brand:
APS LABS - Catalog No.:
APS-BL-26 - Quantity/Unit: 500ml/Pack
- Usually Shipped in: 4-7 Days
Acrylamide/ Bisacrylamide solution 40% (29 :1), 2500ml
₹ 5,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-373L - Quantity/Unit: 2×500ml/Pack
- Availability: In stock
Acrylamide/Bisacrylamide solution 40% (19 :1), 2500ml
₹ 5,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-374L - Quantity/Unit: 2×500ml/Pack
- Availability: In stock
Agar-Agar, 500g
₹ 3,300.00
- Brand:
Aura Biotech - Catalog No.:
ABT-452M - Quantity/Unit: 500g/Pack
- Availability: In stock
Agar-Agar, 1000g
₹ 6,100.00
- Brand:
Aura Biotech - Catalog No.:
ABT-452L - Quantity/Unit: 1000g/Pack
- Availability: In stock
Acrylamide crystals. extra pure AR, 99.9%, 100g
₹ 350.00
- Brand:
Aura Biotech - Catalog No.:
ABT-453S - Quantity/Unit: 100g/Pack
- Availability: In stock
Acrylamide crystals. extra pure AR, 99.9%, 500g
₹ 1,150.00
- Brand:
Aura Biotech - Catalog No.:
ABT-453M - Quantity/Unit: 500g/Pack
- Availability: In stock
Acrylamide crystals. extra pure AR, 99.9%, 2500g
₹ 2,000.00
- Brand:
Aura Biotech - Catalog No.:
ABT-453L - Quantity/Unit: 2×500g/Pack
- Availability: In stock
N, N-Methylene Bisacrylamide (bis-Acrylamide), 100g
₹ 1,140.00
- Brand:
Aura Biotech - Catalog No.:
ABT-454S - Quantity/Unit: 100g/Pack
- Availability: In stock
N, N-Methylene Bisacrylamide (bis-Acrylamide), 500g
₹ 3,000.00
- Brand:
Aura Biotech - Catalog No.:
ABT-454M - Quantity/Unit: 500g/Pack
- Availability: In stock
N, N-Methylene Bisacrylamide (bis-Acrylamide), 2500g
₹ 5,700.00
- Brand:
Aura Biotech - Catalog No.:
ABT-454L - Quantity/Unit: 2×500g/Pack
- Availability: In stock
Ethidium bromide for molecular biology, 1g
₹ 736.00
- Brand:
Aura Biotech - Catalog No.:
ABT-467S - Quantity/Unit: 1g/Pack
- Availability: In stock
Ethidium bromide for molecular biology, 5g
₹ 2,908.00
- Brand:
Aura Biotech - Catalog No.:
ABT-467M - Quantity/Unit: 5g/Pack
- Availability: In stock
Ethidium bromide for molecular biology, 10g
₹ 5,300.00
- Brand:
Aura Biotech - Catalog No.:
ABT-467L - Quantity/Unit: 10g/Pack
- Availability: In stock
Coomassie Brilliant Blue stain, 500ml
₹ 2,200.00
- Brand:
Aura Biotech - Catalog No.:
ABT-385M - Quantity/Unit: 500ml/Pack
- Availability: In stock
Coomassie Brilliant Blue stain, 1000ml
₹ 4,000.00
- Brand:
Aura Biotech - Catalog No.:
ABT-385L - Quantity/Unit: 1000ml/Pack
- Availability: In stock
Aura Pure Agarose Gel Extraction Kit (Column Based), 100 Reactions
₹ 13,000.00
- Brand:
Aura Biotech - Catalog No.:
ABT-173L - Quantity/Unit: 100 Reactions/Pack
- Availability: In stock
50 bp DNA Ladder , 100 wells
₹ 5,500.00
- Brand:
Aura Biotech - Catalog No.:
ABT-121M - Quantity/Unit: 100 wells/Pack
- Availability: In stock
50 bp DNA Ladder , 200 wells
₹ 10,500.00
- Brand:
Aura Biotech - Catalog No.:
ABT-121L - Quantity/Unit: 200 wells/Pack
- Availability: In stock
100 bp DNA Ladder (100 to 1500 bp), 200 wells
₹ 7,500.00
- Brand:
Aura Biotech - Catalog No.:
ABT-122L - Quantity/Unit: 200 wells/Pack
- Availability: In stock