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Polymerases
Polymerase refers to the category of enzymes which catalyze the synthesis of nucleic acid (DNA/RNA) by linking the monomer which in this case is nucleotides, the building blocks of nucleic acids. Depending upon the nucleic acid one is working with polymerase could be DNA or RNA specific. In PCR, a synthetic polymerase such as Taq polymerase is commonly used because of its heat resistant ability. Since Taq is active at room temperature as well it can result in non-specific amplification. In order to minimize this, a Hot start PCR polymerase is used which inactivates Taq at lower temperatures increasing the efficiency of PCR. Reverse transcriptase is another example of the naturally occurring polymerase. It exists in RNA retrovirus (like HIV for example) and synthesizes complementary strand to the preexisting strand of viral RNA. RNA-dependent RNA polymerase (also known as RNA replicase) catalyzes replication of RNA from RNA template. DNA dependent RNA polymerase, on the other hand, catalyzes the transcription of RNA from a DNA template. Another type is a Long-range PCR polymerase which can amplify longer than usual DNA length.
The polymerase is different for prokaryotic and eukaryotic systems. Polymerases are valuable reagents and must be stored and handled with care.
PRODUCTS
ABgene Taq DNA Polymerase with 10x Buffer separate 25 mM MgCl2
ABgene Taq DNA Polymerase with 10x Buffer separate 25 mM MgCl2
₹ 96,270.00
96270
Brand:
Catalog No.:
Quantity/Unit: 4000 x 25 µL rxns/Vial
ABgene Taq DNA Polymerase with 10x Buffer separate 25 mM MgCl2-AB0192A
ABgene Taq DNA Polymerase with 10x Buffer separate 25 mM MgCl2-AB0192A
₹ 9,510.00
9510
Brand:
Catalog No.:
Quantity/Unit: 400 x 25 µL rxns/Vial
ABgene Taq DNA Polymerase with 10x Buffer containing 15 mM MgCl2
ABgene Taq DNA Polymerase with 10x Buffer containing 15 mM MgCl2
₹ 96,150.00
96150
Brand:
Catalog No.:
Quantity/Unit: 4000 x 25 µL rxns/Vial
ABgene Taq DNA Polymerase with 10x Buffer containing 15 mM MgCl2-AB1192A
ABgene Taq DNA Polymerase with 10x Buffer containing 15 mM MgCl2-AB1192A
₹ 9,520.00
9520
Brand:
Catalog No.:
Quantity/Unit: 400 x 25 µL rxns/Vial
DyNAzyme EXT DNA Polymerase
DyNAzyme EXT DNA Polymerase
₹ 50,260.00
50260
Brand:
Catalog No.:
Quantity/Unit: 1000 Unit/Vial
DyNAzyme II DNA Polymerase
DyNAzyme II DNA Polymerase
₹ 3,160.00
3160
Brand:
Catalog No.:
Quantity/Unit: 1000 Unit/Vial
DyNAzyme II DNA Polymerase Mg-free Buffer
DyNAzyme II DNA Polymerase Mg-free Buffer
₹ 21,090.00
21090
Brand:
Catalog No.:
Quantity/Unit: 1000 Unit/Vial
Extensor Long Range PCR Enzyme
Extensor Long Range PCR Enzyme
₹ 54,070.00
54070
Brand:
Catalog No.:
Quantity/Unit: 800 x 25 µL rxns/Vial
Phi6 RNA Replicase (1 U/µL)
Phi6 RNA Replicase (1 U/µL)
₹ 48,270.00
48270
Brand:
Catalog No.:
Quantity/Unit: 300U/Vial
