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Polymerases

Polymerase refers to the category of enzymes which catalyze the synthesis of nucleic acid (DNA/RNA) by linking the monomer which in this case is nucleotides, the building blocks of nucleic acids. Depending upon the nucleic acid one is working with polymerase could be DNA or RNA specific. In PCR, a synthetic polymerase such as Taq polymerase is commonly used because of its heat resistant ability. Since Taq is active at room temperature as well it can result in non-specific amplification. In order to minimize this, a Hot start PCR polymerase is used which inactivates Taq at lower temperatures increasing the efficiency of PCR. Reverse transcriptase is another example of the naturally occurring polymerase. It exists in RNA retrovirus (like HIV for example) and synthesizes complementary strand to the preexisting strand of viral RNA. RNA-dependent RNA polymerase (also known as RNA replicase) catalyzes replication of RNA from RNA template. DNA dependent RNA polymerase, on the other hand, catalyzes the transcription of RNA from a DNA template. Another type is a Long-range PCR polymerase which can amplify longer than usual DNA length.

The polymerase is different for prokaryotic and eukaryotic systems. Polymerases are valuable reagents and must be stored and handled with care.

PRODUCTS

ABgene Taq DNA Polymerase with 10x Buffer separate 25 mM MgCl2

ABgene Taq DNA Polymerase with 10x Buffer separate 25 mM MgCl2

₹ 96,270.00 96270

Brand: ABgene

Catalog No.: AB0192B

Quantity/Unit: 4000 x 25 µL rxns/Vial

ABgene Taq DNA Polymerase with 10x Buffer containing 15 mM MgCl2

ABgene Taq DNA Polymerase with 10x Buffer containing 15 mM MgCl2

₹ 96,150.00 96150

Brand: ABgene

Catalog No.: AB1192B

Quantity/Unit: 4000 x 25 µL rxns/Vial

DyNAzyme EXT DNA Polymerase

DyNAzyme EXT DNA Polymerase

₹ 50,260.00 50260

Brand: Finnzymes

Catalog No.: FNZ505L

Quantity/Unit: 1000 Unit/Vial

DyNAzyme II DNA Polymerase

DyNAzyme II DNA Polymerase

₹ 3,160.00 3160

Brand: Finnzymes

Catalog No.: FNZ501L

Quantity/Unit: 1000 Unit/Vial

DyNAzyme II DNA Polymerase Mg-free Buffer

DyNAzyme II DNA Polymerase Mg-free Buffer

₹ 21,090.00 21090

Brand: Finnzymes

Catalog No.: FNZ503L

Quantity/Unit: 1000 Unit/Vial

Extensor Long Range PCR Enzyme

Extensor Long Range PCR Enzyme

₹ 54,070.00 54070

Brand: ABgene

Catalog No.: AB0720B

Quantity/Unit: 800 x 25 µL rxns/Vial

Phi6 RNA Replicase (1 U/µL)

Phi6 RNA Replicase (1 U/µL)

₹ 48,270.00 48270

Brand: Finnzymes

Catalog No.: FNZ611L

Quantity/Unit: 300U/Vial