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Cloning

It is a molecular biology technique wherein a selected sequence of DNA is cloned into multiple copies in a host organism (usually bacteria). A vector is required to introduce the DNA sequence of interest into the host. This vector in most cases is a plasmid, especially containing an antibiotic resistance gene. Plasmids are treated with restriction enzymes which cleave the plasmid DNA at a specific region called as the restriction sites. This cleavage usually leads to the production of single-stranded sticky ends or overhangs which can anneal with the foreign DNA. This annealing process is referred to as ligation/hybridization because it occurs with the action of DNA ligase. The plasmid is now known as a recombinant plasmid. The bacterial cells (such asE. coli) which have been treated with calcium chloride to make them more permeable to DNA molecules are added to the recombinant plasmid mixture. By the process known as transformation, bacterial cells take up the recombinant plasmid. These bacterial cells are then grown on the nutrient agar containing a specific antibiotic. After incubation, this will result in the growth of only those cells which have taken the antibiotic resistance gene in plasmid, suggesting that these cells have taken up the recombinant DNA. Theplasmid replicates by itself, independent of the host replication. However when host cells divide each of the daughter cells receives copies of plasmids. This results in the production of large copies of the recombinant plasmid.
Separate components or Ready-to-use Kits are available toassist plasmid preparation, ligation, transformation, and plasmid purification.

PRODUCTS

CHAPS, Molecular Biology Grade-3602000051730

CHAPS, Molecular Biology Grade-3602000051730

₹ 2,940.00

Brand: Bangalore GeNei

Catalog No.: 3602000051730

Quantity/Unit: 5 g/Pack

Usually Shipped in: 2-3 Weeks

CloneJET PCR Cloning Kit, 20 rxn

CloneJET PCR Cloning Kit, 20 rxn

₹ 12,120.00

Brand: Fermentas

Catalog No.: K1231

Quantity/Unit: 20 rxn/Kit

Usually Shipped in: 1-2 Weeks

CloneJET PCR Cloning Kit, 40 rxn

CloneJET PCR Cloning Kit, 40 rxn

₹ 23,098.00

Brand: Fermentas

Catalog No.: K1232

Quantity/Unit: 40 rxn/Kit

Usually Shipped in: 1-2 Weeks

DNA Ligation Kit , Mighty Mix

DNA Ligation Kit , Mighty Mix

₹ 9,161.00

Brand: Takara Bio

Catalog No.: 6023

Quantity/Unit: 1 Kit/Pack

Availability: In stock

E. coli DH5α Competent Cells

E. coli DH5α Competent Cells

₹ 10,039.00

Brand: Takara Bio

Catalog No.: 9057

Quantity/Unit: 100 ul x 10 rxn/Pack

Availability: In stock

E. coli JM109 Competent Cells

E. coli JM109 Competent Cells

₹ 8,031.00

Brand: Takara Bio

Catalog No.: 9052

Quantity/Unit: 100ul x 10 rxn/Pack

Availability: In stock

EBR/GeneJET Endo-free

EBR/GeneJET Endo-free

₹ 8,343.46

Brand: Fermentas

Catalog No.: R2251

Quantity/Unit: 12 mL/Bottle

Usually Shipped in: 1-2 Weeks

Elution Buf/GeneJET Endo-free

Elution Buf/GeneJET Endo-free

₹ 2,859.15

Brand: Fermentas

Catalog No.: R2281

Quantity/Unit: 18 mL/Bottle

Usually Shipped in: 1-2 Weeks

Elution Buffer, 30 mL

Elution Buffer, 30 mL

₹ 3,067.05

Brand: Fermentas

Catalog No.: R1263

Quantity/Unit: 30 mL/Bottle

Usually Shipped in: 1-2 Weeks

Fast DNA End Repair Kit, 250 prep

Fast DNA End Repair Kit, 250 prep

₹ 14,578.62

Brand: Fermentas

Catalog No.: K0771

Quantity/Unit: 250 prep/Kit

Usually Shipped in: 1-2 Weeks

GeNei In-Vitro Transcription Teaching Kit-6113100011730

GeNei In-Vitro Transcription Teaching Kit-6113100011730

₹ 7,820.00

Brand: Bangalore GeNei

Catalog No.: 6113100011730

Quantity/Unit: 10 expts. /Kit

Usually Shipped in: 2-3 Weeks

GeNei Bacterial Gene Expression Teaching Kit-6106900011730

GeNei Bacterial Gene Expression Teaching Kit-6106900011730

₹ 9,520.00

Brand: Bangalore GeNei

Catalog No.: 6106900011730

Quantity/Unit: 5 expts. /Kit

Usually Shipped in: 2-3 Weeks

GeNei Competent Cells Preparation Kit (A)-1660100011730

GeNei Competent Cells Preparation Kit (A)-1660100011730

₹ 4,650.00

Brand: Bangalore GeNei

Catalog No.: 1660100011730

Quantity/Unit: 50 reactions/Vial

Usually Shipped in: 2-3 Weeks

GeNei Competent Cells Preparation Kit (B)-1660200011730

GeNei Competent Cells Preparation Kit (B)-1660200011730

₹ 5,820.00

Brand: Bangalore GeNei

Catalog No.: 1660200011730

Quantity/Unit: 50 reactions/Vial

Usually Shipped in: 2-3 Weeks

GeNei GFP Cloning Teaching Kit-6106000011730

GeNei GFP Cloning Teaching Kit-6106000011730

₹ 6,600.00

Brand: Bangalore GeNei

Catalog No.: 6106000011730

Quantity/Unit: 5 expts. /Kit

Usually Shipped in: 2-3 Weeks