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Cloning

Cloning

It is a molecular biology technique wherein a selected sequence of DNA is cloned into multiple copies in a host organism (usually bacteria). A vector is required to introduce the DNA sequence of interest into the host. This vector in most cases is a plasmid, especially containing an antibiotic resistance gene. Plasmids are treated with restriction enzymes which cleave the plasmid DNA at a specific region called as the restriction sites. This cleavage usually leads to the production of single-stranded sticky ends or overhangs which can anneal with the foreign DNA. This annealing process is referred to as ligation/hybridization because it occurs with the action of DNA ligase. The plasmid is now known as a recombinant plasmid. The bacterial cells (such asE. coli) which have been treated with calcium chloride to make them more permeable to DNA molecules are added to the recombinant plasmid mixture. By the process known as transformation, bacterial cells take up the recombinant plasmid. These bacterial cells are then grown on the nutrient agar containing a specific antibiotic. After incubation, this will result in the growth of only those cells which have taken the antibiotic resistance gene in plasmid, suggesting that these cells have taken up the recombinant DNA. Theplasmid replicates by itself, independent of the host replication. However when host cells divide each of the daughter cells receives copies of plasmids. This results in the production of large copies of the recombinant plasmid.
Separate components or Ready-to-use Kits are available toassist plasmid preparation, ligation, transformation, and plasmid purification.
MOPS Sodium Salt, Molecular Biology Grade-3601905001730

Brand: Bangalore GeNei

₹ 19,668.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3601905001730
  • Quantity/Unit: 500 g/Pack
pUC19 (Cesium Chloride Purified)-1600370501730

Brand: Bangalore GeNei

₹ 7,447.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1600370501730
  • Quantity/Unit: 50µg/Vial
pUC19-1600470501730

Brand: Bangalore GeNei

₹ 5,984.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1600470501730
  • Quantity/Unit: 50µg/Vial
HEPES Sodium Salt, Molecular Biology Grade-3601505001730

Brand: Bangalore GeNei

₹ 17,028.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3601505001730
  • Quantity/Unit: 500 g/Pack
pUC18-1600270101730

Brand: Bangalore GeNei

₹ 2,332.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1600270101730
  • Quantity/Unit: 10µg/Vial
pBR322 (Cesium Chloride Purified)-1600570501730

Brand: Bangalore GeNei

₹ 10,120.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1600570501730
  • Quantity/Unit: 50µg/Vial
Tris-Base, Molecular Biology Grade-3600710001730

Brand: Bangalore GeNei

₹ 8,503.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3600710001730
  • Quantity/Unit: 1 Kg/Pack
pUC18-1600270501730

Brand: Bangalore GeNei

₹ 5,984.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1600270501730
  • Quantity/Unit: 50µg/Vial
pUC18 (Cesium Chloride Purified)-1600170501730

Brand: Bangalore GeNei

₹ 7,447.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1600170501730
  • Quantity/Unit: 50µg/Vial
Tris-HCl, Molecular Biology Grade-3601310001730

Brand: Bangalore GeNei

₹ 10,197.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3601310001730
  • Quantity/Unit: 1 Kg/Pack
Lambda DNA-1600773001730

Brand: Bangalore GeNei

₹ 5,665.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1600773001730
  • Quantity/Unit: 300µg/Vial
GeNei GFP Cloning Teaching Kit-6106000011730

Brand: Bangalore GeNei

₹ 8,349.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6106000011730
  • Quantity/Unit: 5 expts./Kit
GeNei Plasmid Preparation Teaching Kit-6100600021730

Brand: Bangalore GeNei

₹ 13,288.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6100600021730
  • Quantity/Unit: 20 expts./Kit
GeNei Plasmid Preparation Teaching Kit (5 x 5 preps)

Brand: Bangalore GeNei

₹ 6,831.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6100600011730
  • Quantity/Unit: 25 experiments/Kit
GeNei Instant Cloning Kit-1660630011730

Brand: Bangalore GeNei

₹ 9,306.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1660630011730
  • Quantity/Unit: 10 reactions/Vial
GeNei Competent Cells Preparation Kit (A)-1660100011730

Brand: Bangalore GeNei

₹ 5,885.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1660100011730
  • Quantity/Unit: 50 reactions/Vial
GeNei Competent Cells Preparation Kit (B)-1660200011730

Brand: Bangalore GeNei

₹ 7,370.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1660200011730
  • Quantity/Unit: 50 reactions/Vial
GeNei Transformation Teaching Kit-6100700011730

Brand: Bangalore GeNei

₹ 6,974.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6100700011730
  • Quantity/Unit: 5 expts./Kit
GeNei Transformation Teaching Kit-6100700021730

Brand: Bangalore GeNei

₹ 17,941.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6100700021730
  • Quantity/Unit: 20 expts./Kit
GeNei Bacterial Gene Expression Teaching Kit-6106900011730

Brand: Bangalore GeNei

₹ 12,045.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6106900011730
  • Quantity/Unit: 5 expts./Kit
GeNei In-Vitro Transcription Teaching Kit-6113100011730

Brand: Bangalore GeNei

₹ 9,900.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6113100011730
  • Quantity/Unit: 10 expts./Kit
Bacterial Gene expression teaching kit

Brand: Aura Biotech

₹ 5,500.00
  • Brand: Aura Biotech
  • Catalog No.: MPL-11
  • Quantity/Unit: 10 Rxns/Pack
  • Usually Shipped in: 1-3 Days
pBR322-1600670501730

Brand: Bangalore GeNei

₹ 6,380.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1600670501730
  • Quantity/Unit: 50µg/Vial
HEPES, Molecular Biology Grade-3601405001730

Brand: Bangalore GeNei

₹ 10,923.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3601405001730
  • Quantity/Unit: 500 g/Pack
CHAPS, Molecular Biology Grade-3602000051730

Brand: Bangalore GeNei

₹ 3,729.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3602000051730
  • Quantity/Unit: 5 g/Pack
PIPES, Molecular Biology Grade-3601605001730

Brand: Bangalore GeNei

₹ 13,288.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3601605001730
  • Quantity/Unit: 500 g/Pack
pBR322-1600670101730

Brand: Bangalore GeNei

₹ 2,332.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1600670101730
  • Quantity/Unit: 10µg/Vial
Lambda DNA-1600770601730

Brand: Bangalore GeNei

₹ 1,914.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1600770601730
  • Quantity/Unit: 60µg/Vial