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Cloning

Cloning

It is a molecular biology technique wherein a selected sequence of DNA is cloned into multiple copies in a host organism (usually bacteria). A vector is required to introduce the DNA sequence of interest into the host. This vector in most cases is a plasmid, especially containing an antibiotic resistance gene. Plasmids are treated with restriction enzymes which cleave the plasmid DNA at a specific region called as the restriction sites. This cleavage usually leads to the production of single-stranded sticky ends or overhangs which can anneal with the foreign DNA. This annealing process is referred to as ligation/hybridization because it occurs with the action of DNA ligase. The plasmid is now known as a recombinant plasmid. The bacterial cells (such asE. coli) which have been treated with calcium chloride to make them more permeable to DNA molecules are added to the recombinant plasmid mixture. By the process known as transformation, bacterial cells take up the recombinant plasmid. These bacterial cells are then grown on the nutrient agar containing a specific antibiotic. After incubation, this will result in the growth of only those cells which have taken the antibiotic resistance gene in plasmid, suggesting that these cells have taken up the recombinant DNA. Theplasmid replicates by itself, independent of the host replication. However when host cells divide each of the daughter cells receives copies of plasmids. This results in the production of large copies of the recombinant plasmid.
Separate components or Ready-to-use Kits are available toassist plasmid preparation, ligation, transformation, and plasmid purification.
EBR/GeneJET Endo-free

Brand: Fermentas

₹ 12,197.00
  • Brand: Fermentas
  • Catalog No.: R2251
  • Quantity/Unit: 12 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Wash Buf1/GeneJET Endo-free

Brand: Fermentas

₹ 4,140.00
  • Brand: Fermentas
  • Catalog No.: R2261
  • Quantity/Unit: 75 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Wash Buf2/GeneJET Endo-free

Brand: Fermentas

₹ 4,140.00
  • Brand: Fermentas
  • Catalog No.: R2271
  • Quantity/Unit: 75 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Elution Buf/GeneJET Endo-free

Brand: Fermentas

₹ 4,140.00
  • Brand: Fermentas
  • Catalog No.: R2281
  • Quantity/Unit: 18 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
IPTG dioxane-free, 1 gram

Brand: Fermentas

  • Brand: Fermentas
  • Catalog No.: R0391
  • Quantity/Unit: 1 gram/Pack
  • Usually Shipped in: 1-2 Weeks
IPTG dioxane-free, 5 gram

Brand: Fermentas

₹ 14,103.00
  • Brand: Fermentas
  • Catalog No.: R0392
  • Quantity/Unit: 5 gram/Pack
  • Usually Shipped in: 1-2 Weeks
IPTG dioxane-free, 5 x 5 gram

Brand: Fermentas

₹ 47,476.00
  • Brand: Fermentas
  • Catalog No.: R0393
  • Quantity/Unit: 5 x 5 gram/Pack
  • Usually Shipped in: 1-2 Weeks
X-Gal, 0.5 gram

Brand: Fermentas

  • Brand: Fermentas
  • Catalog No.: R0401
  • Quantity/Unit: 0.5 gram/Pack
  • Usually Shipped in: 1-2 Weeks
X-Gal, 2 x 1 gram

Brand: Fermentas

₹ 24,247.00
  • Brand: Fermentas
  • Catalog No.: R0402
  • Quantity/Unit: 2 x 1 gram/Pack
  • Usually Shipped in: 1-2 Weeks
X-Gal, 1 gram

Brand: Fermentas

₹ 14,772.00
  • Brand: Fermentas
  • Catalog No.: R0404
  • Quantity/Unit: 1 gram/Pack
  • Usually Shipped in: 1-2 Weeks
X-Gal Solution ready-to-use, 10 mL

Brand: Fermentas

₹ 17,046.00
  • Brand: Fermentas
  • Catalog No.: R0941
  • Quantity/Unit: 10 mL/Pack
  • Usually Shipped in: 1-2 Weeks
IPTG Solution ready-to-use, 10 x 1.5 mL

Brand: Fermentas

₹ 9,360.00
  • Brand: Fermentas
  • Catalog No.: R1171
  • Quantity/Unit: 10 x 1.5 mL/Pack
  • Usually Shipped in: 1-2 Weeks
Resuspension Solution, 70 mL

Brand: Fermentas

₹ 5,674.00
  • Brand: Fermentas
  • Catalog No.: R1213
  • Quantity/Unit: 70 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Lysis Solution , 70 mL

Brand: Fermentas

₹ 6,204.00
  • Brand: Fermentas
  • Catalog No.: R1223
  • Quantity/Unit: 70 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Neutralization Solution, 100 mL

Brand: Fermentas

₹ 5,674.00
  • Brand: Fermentas
  • Catalog No.: R1233
  • Quantity/Unit: 100 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Wash Solution , 100 mL

Brand: Fermentas

₹ 6,414.00
  • Brand: Fermentas
  • Catalog No.: R1243
  • Quantity/Unit: 100 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Elution Buffer, 30 mL for GeneJET Plasmid Miniprep Kit

Brand: Fermentas

₹ 4,440.00
  • Brand: Fermentas
  • Catalog No.: R1263
  • Quantity/Unit: 30 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Resuspension Solution (for kits K0491/2)

Brand: Fermentas

₹ 7,340.00
  • Brand: Fermentas
  • Catalog No.: R1581
  • Quantity/Unit: 240 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Resusp. Sol/GeneJET Endo-free

Brand: Fermentas

₹ 6,343.00
  • Brand: Fermentas
  • Catalog No.: R2221
  • Quantity/Unit: 84 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Lysis Sol/GeneJET Endo-free

Brand: Fermentas

₹ 6,315.00
  • Brand: Fermentas
  • Catalog No.: R2231
  • Quantity/Unit: 84 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Neutral. Sol/GeneJET Endo-free

Brand: Fermentas

₹ 4,918.00
  • Brand: Fermentas
  • Catalog No.: R2241
  • Quantity/Unit: 84 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
pUC57 DNA, 50 g

Brand: Fermentas

₹ 6,212.00
  • Brand: Fermentas
  • Catalog No.: SD0171
  • Quantity/Unit: 50 µg/Vial
  • Usually Shipped in: 1-2 Weeks
Fast DNA End Repair Kit, 250 prep

Brand: Fermentas

₹ 21,108.00
  • Brand: Fermentas
  • Catalog No.: K0771
  • Quantity/Unit: 250 prep/Kit
  • Usually Shipped in: 1-2 Weeks
InsTAclone PCR Cloning Kit, 10 rxn

Brand: Fermentas

  • Brand: Fermentas
  • Catalog No.: K1213
  • Quantity/Unit: 10 rxn/Kit
  • Usually Shipped in: 1-2 Weeks
CloneJET PCR Cloning Kit, 20 rxn

Brand: Fermentas

₹ 17,547.00
  • Brand: Fermentas
  • Catalog No.: K1231
  • Quantity/Unit: 20 rxn/Kit
  • Usually Shipped in: 1-2 Weeks
CloneJET PCR Cloning Kit, 40 rxn

Brand: Fermentas

₹ 33,443.00
  • Brand: Fermentas
  • Catalog No.: K1232
  • Quantity/Unit: 40 rxn/Kit
  • Usually Shipped in: 1-2 Weeks
aLICator LIC Cloning and Expression Kit 1 (untagged) , 20 rxn

Brand: Fermentas

₹ 23,367.00
  • Brand: Fermentas
  • Catalog No.: K1241
  • Quantity/Unit: 20 rxn/Kit
  • Usually Shipped in: 1-2 Weeks
aLICator LIC Cloning and Expression Kit 2 (N-terminal His-tag/EK) , 20 rxn

Brand: Fermentas

₹ 27,969.00
  • Brand: Fermentas
  • Catalog No.: K1251
  • Quantity/Unit: 20 rxn/Kit
  • Usually Shipped in: 1-2 Weeks