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Cloning

It is a molecular biology technique wherein a selected sequence of DNA is cloned into multiple copies in a host organism (usually bacteria). A vector is required to introduce the DNA sequence of interest into the host. This vector in most cases is a plasmid, especially containing an antibiotic resistance gene. Plasmids are treated with restriction enzymes which cleave the plasmid DNA at a specific region called as the restriction sites. This cleavage usually leads to the production of single-stranded sticky ends or overhangs which can anneal with the foreign DNA. This annealing process is referred to as ligation/hybridization because it occurs with the action of DNA ligase. The plasmid is now known as a recombinant plasmid. The bacterial cells (such as E. coli) which have been treated with calcium chloride to make them more permeable to DNA molecules are added to the recombinant plasmid mixture. By the process known as transformation, bacterial cells take up the recombinant plasmid. These bacterial cells are then grown on the nutrient agar containing a specific antibiotic. After incubation, this will result in the growth of only those cells which have taken the antibiotic resistance gene in plasmid, suggesting that these cells have taken up the recombinant DNA. The plasmid replicates by itself, independent of the host replication. However when host cells divide each of the daughter cells receives copies of plasmids. This results in the production of large copies of the recombinant plasmid.

Separate components or Ready-to-use Kits are available to assist plasmid preparation, ligation, transformation, and plasmid purification. 

PRODUCTS

GFP cloning teaching kit 5 exps

GFP cloning teaching kit 5 exps

₹ 7,062.00 7062

Brand: Bangalore GeNei

Catalog No.: 616106000011730

Quantity/Unit: 5 experiments/Kit

Instant Cloning Kit 10 reactions

Instant Cloning Kit 10 reactions

₹ 7,865.00 7865

Brand: Bangalore GeNei

Catalog No.: 611660400011730

Quantity/Unit: 10 reactions/Kit

Instant ligation kit 20 reactions

Instant ligation kit 20 reactions

₹ 4,382.00 4382

Brand: Bangalore GeNei

Catalog No.: 611660300011730

Quantity/Unit: 20 reactions/Kit

Lambda DNA 300ug

Lambda DNA 300ug

₹ 5,100.00 5100

Brand: Bangalore GeNei

Catalog No.: 611600773001730

Quantity/Unit: 300 ug/Vial

Availability: In stock

Lambda DNA 60ug

Lambda DNA 60ug

₹ 1,616.00 1616

Brand: Bangalore GeNei

Catalog No.: 611600770601730

Quantity/Unit: 60 ug/Vial

Lambda DNA/EcoR 50ug

Lambda DNA/EcoR 50ug

₹ 2,429.00 2429

Brand: Bangalore GeNei

Catalog No.: 612660370501730

Quantity/Unit: 50 ug/Vial

Lambda DNA/Hind III Digest, 50 μg (RTU)

Lambda DNA/Hind III Digest, 50 μg (RTU)

₹ 2,450.00 2450

Brand: Bangalore GeNei

Catalog No.: 612660270501730

Quantity/Unit: 50 ug/Vial

Ligation kit 20exp

Ligation kit 20exp

₹ 9,800.00 9800

Brand: Bangalore GeNei

Catalog No.: 616100800021730

Quantity/Unit: 20 experiments/Kit

Ligation kit 5exp

Ligation kit 5exp

₹ 3,809.00 3809

Brand: Bangalore GeNei

Catalog No.: 616100800011730

Quantity/Unit: 5 experiments/Kit