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Cloning
It is a molecular biology technique wherein a selected sequence of DNA is cloned into multiple copies in a host organism (usually bacteria). A vector is required to introduce the DNA sequence of interest into the host. This vector in most cases is a plasmid, especially containing an antibiotic resistance gene. Plasmids are treated with restriction enzymes which cleave the plasmid DNA at a specific region called as the restriction sites. This cleavage usually leads to the production of single-stranded sticky ends or overhangs which can anneal with the foreign DNA. This annealing process is referred to as ligation/hybridization because it occurs with the action of DNA ligase. The plasmid is now known as a recombinant plasmid. The bacterial cells (such as E. coli) which have been treated with calcium chloride to make them more permeable to DNA molecules are added to the recombinant plasmid mixture. By the process known as transformation, bacterial cells take up the recombinant plasmid. These bacterial cells are then grown on the nutrient agar containing a specific antibiotic. After incubation, this will result in the growth of only those cells which have taken the antibiotic resistance gene in plasmid, suggesting that these cells have taken up the recombinant DNA. The plasmid replicates by itself, independent of the host replication. However when host cells divide each of the daughter cells receives copies of plasmids. This results in the production of large copies of the recombinant plasmid.
Separate components or Ready-to-use Kits are available to assist plasmid preparation, ligation, transformation, and plasmid purification.
PRODUCTS
GFP cloning teaching kit 5 exps
GFP cloning teaching kit 5 exps
₹ 7,062.00
7062
Brand:
Catalog No.:
Quantity/Unit: 5 experiments/Kit
Instant Cloning Kit 10 reactions
Instant Cloning Kit 10 reactions
₹ 7,865.00
7865
Brand:
Catalog No.:
Quantity/Unit: 10 reactions/Kit
Instant ligation kit 20 reactions
Instant ligation kit 20 reactions
₹ 4,382.00
4382
Brand:
Catalog No.:
Quantity/Unit: 20 reactions/Kit
Lambda DNA 300ug
Lambda DNA 300ug
₹ 5,100.00
5100
Brand:
Catalog No.:
Quantity/Unit: 300 ug/Vial
Availability: In stock
Lambda DNA 60ug
Lambda DNA 60ug
₹ 1,616.00
1616
Brand:
Catalog No.:
Quantity/Unit: 60 ug/Vial
Lambda DNA/EcoR 50ug
Lambda DNA/EcoR 50ug
₹ 2,429.00
2429
Brand:
Catalog No.:
Quantity/Unit: 50 ug/Vial
Lambda DNA/Hind III Digest, 50 μg (RTU)
Lambda DNA/Hind III Digest, 50 μg (RTU)
₹ 2,450.00
2450
Brand:
Catalog No.:
Quantity/Unit: 50 ug/Vial
Ligation kit 20exp
Ligation kit 20exp
₹ 9,800.00
9800
Brand:
Catalog No.:
Quantity/Unit: 20 experiments/Kit
Ligation kit 5exp
Ligation kit 5exp
₹ 3,809.00
3809
Brand:
Catalog No.:
Quantity/Unit: 5 experiments/Kit
