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Protein Extraction

The process of Protein extraction would broadly follow tissue/cell lysis (extraction), precipitation and then purification. The step one may involve the use of blender, sonicator or a chemical method of enzymatic digestion. The goal is to pool out the proteins from the cell membrane via breaking the membrane. Cell lysis normally releases protease which may break down the protein of interest. In order to avoid this, protease inhibitors are used and next step is made quickly.
Many methods can be employed to precipitate the proteins out. One common way is to use Ammonium sulfate wherein the solubility of protein gets altered in presence of the high salt concentration. This method is also known as salting out since ammonium sulfate is a neutral salt. If a strong detergent like Sodium Dodecyl Sulfate (SDS) is used in the extraction step then most membrane proteins will precipitate upon removal of the detergent as membrane proteins are soluble in SDS. Miscible solvents like methanol and ethanol are also used for precipitation as their addition decreases the dielectric constant of water, which in turn allows two proteins to come close together and precipitate. By this point, the protein might have some Non-compacted particles which are removed as 'supernatant' through the process of centrifugation.
Many commercial kits offer reagents for quicker and easier protein extraction. Biomall offers a range of such kits along with all other reagents and solutions required in protein extraction procedures.

PRODUCTS

AB-1171 ATTOPREP MF

AB-1171 ATTOPREP MF

Brand: BioSpan

Catalog No.: 3521370

Quantity/Unit: 50 columns/Box

Availability: In stock

AE-1435 EzApply 2D kit

AE-1435 EzApply 2D kit

Brand: BioSpan

Catalog No.: 2332335

Quantity/Unit: 1 kit /Box

Availability: In stock

Accutase Cell Detachment Solution

Accutase Cell Detachment Solution

Brand: BD Biosciences

Catalog No.: 561527

Quantity/Unit: 100 ml/Bottle

Usually Shipped in: 3-4 Weeks

Annexin V FITC Apop Dtec Kit I

Annexin V FITC Apop Dtec Kit I

Brand: BD Biosciences

Catalog No.: 556547

Quantity/Unit: 100 Tests/Kits

Usually Shipped in: 3-4 Weeks

B-PER(Enzymes Bacterial Protein Extraction Kit)

B-PER(Enzymes Bacterial Protein Extraction Kit)

₹ 89,411.00

Brand: Pierce Protein Research Products

Catalog No.: PI90079

Quantity/Unit: 500 ml/Kit

Usually Shipped in: 3-4 Weeks

BD FACS Shutdown Solution

BD FACS Shutdown Solution

Brand: BD Biosciences

Catalog No.: 334224

Quantity/Unit: 5 Litres/Bottle

Usually Shipped in: 3-4 Weeks

CHAPS

CHAPS

₹ 22,160.00

Brand: Pierce Protein Research Products

Catalog No.: PI28300

Quantity/Unit: 5 GM/pack

Usually Shipped in: 3-4 Weeks

Calibrite 3 Color Beads Kit

Calibrite 3 Color Beads Kit

Brand: BD Biosciences

Catalog No.: 340486

Quantity/Unit: 25 Tests/Kit

Usually Shipped in: 3-4 Weeks

Extract-EZ C -BCBSP013-1kit

Extract-EZ C -BCBSP013-1kit

₹ 7,800.00

Brand: BR Biochem Life Sciences

Catalog No.: BCBSP013-1kit

Quantity/Unit: 1kit/Pack

Availability: In stock

Extract-EZ E1 Kit-BCBSP073-50 Preps

Extract-EZ E1 Kit-BCBSP073-50 Preps

₹ 18,201.00

Brand: BR Biochem Life Sciences

Catalog No.: BCBSP073-50 Preps

Quantity/Unit: 50 Preps/Kit

Availability: In stock

Extract-EZ G Kit-BCBSP001-50 Preps

Extract-EZ G Kit-BCBSP001-50 Preps

₹ 16,344.00

Brand: BR Biochem Life Sciences

Catalog No.: BCBSP001-50 Preps

Quantity/Unit: 50 Preps/Kit

Availability: In stock

FACS Clean Solution

FACS Clean Solution

Brand: BD Biosciences

Catalog No.: 340345

Quantity/Unit: 5 L/Bottle

Usually Shipped in: 3-4 Weeks

FFPE Protein Extraction Kit-BCBSP058-50 Preps

FFPE Protein Extraction Kit-BCBSP058-50 Preps

₹ 11,143.00

Brand: BR Biochem Life Sciences

Catalog No.: BCBSP058-50 Preps

Quantity/Unit: 50 Preps/Kit

Availability: In stock

Leupeptin Protease Inhibitor

Leupeptin Protease Inhibitor

₹ 52,123.00

Brand: Pierce Protein Research Products

Catalog No.: PI78435

Quantity/Unit: 50 MG/pack

Usually Shipped in: 3-4 Weeks

Nuclear Protein Fractionation Kit-BCBSP009-50 Preps

Nuclear Protein Fractionation Kit-BCBSP009-50 Preps

₹ 8,358.00

Brand: BR Biochem Life Sciences

Catalog No.: BCBSP009-50 Preps

Quantity/Unit: 50 Preps/Kit

Availability: In stock