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DNA Purification and Extraction

DNA Purification and Extraction

Swiss Biologist Friedrich Miescher was the first person to isolate a DNA. The process refers to both extraction and purification of DNA from the given sample such as blood, swab, plant tissue, animal tissue, bacterial cells, etc. A general protocol involves homogenization of cells using a physical method like the use of mortar and pestle or automated homogenizer. This may be followed by a series of chemical treatment with detergents to remove fats, rupture cellular membranes and then by using Chloroform & further centrifugation, DNA can be separated from the cellular debris. A high temperature may be maintained to promote cell lysis. A buffer like Tris to maintain pH is used throughout. Ethanol or isopropanol is then used to precipitate out DNA. Purification may involve many steps like the use of proteinase or phenol-chloroform extraction to eliminate protein impurities or RNAse to remove RNA.
 
Kits for extraction and purification of DNA specific to the source of DNA are available for ready-to-use, or separate components can be used to develop a standardized protocol.
 
GeneJET Plasmid Miniprep Kit, 250 prep

Brand: Fermentas

₹ 26,146.00
  • Brand: Fermentas
  • Catalog No.: K0503
  • Quantity/Unit: 250 prep/Kit
  • Usually Shipped in: 1-2 Weeks
GeneJET Plasmid Midiprep Kit, 25 prep

Brand: Fermentas

₹ 20,688.00
  • Brand: Fermentas
  • Catalog No.: K0481
  • Quantity/Unit: 25 prep/Kit
  • Usually Shipped in: 1-2 Weeks
GeneJET Plasmid Midiprep Kit, 100 prep

Brand: Fermentas

₹ 74,393.00
  • Brand: Fermentas
  • Catalog No.: K0482
  • Quantity/Unit: 100 prep/Kit
  • Usually Shipped in: 1-2 Weeks
GeneJET Plasmid Maxiprep Kit, 10 prep

Brand: Fermentas

₹ 18,273.00
  • Brand: Fermentas
  • Catalog No.: K0491
  • Quantity/Unit: 10 prep/Kit
  • Usually Shipped in: 1-2 Weeks
GeneJET Plasmid Maxiprep Kit, 25 prep

Brand: Fermentas

₹ 38,095.00
  • Brand: Fermentas
  • Catalog No.: K0492
  • Quantity/Unit: 25 prep/Kit
  • Usually Shipped in: 1-2 Weeks
GeneJET Endo-free Plasmid Maxi Kit, 10 Preps

Brand: Fermentas

  • Brand: Fermentas
  • Catalog No.: K0861
  • Quantity/Unit: 10 Prep/Kit
  • Usually Shipped in: 1-2 Weeks
Genomic DNA Purification Kit, 100 prep

Brand: Fermentas

₹ 13,958.00
  • Brand: Fermentas
  • Catalog No.: K0512
  • Quantity/Unit: 100 prep/Kit
  • Usually Shipped in: 1-2 Weeks
GeneJET Genomic DNA Purification Kit, 50 prep

Brand: Fermentas

₹ 20,944.00
  • Brand: Fermentas
  • Catalog No.: K0721
  • Quantity/Unit: 50 prep/Kit
  • Usually Shipped in: 1-2 Weeks
GeneJET Genomic DNA Purification Kit, 250 prep

Brand: Fermentas

₹ 94,906.00
  • Brand: Fermentas
  • Catalog No.: K0722
  • Quantity/Unit: 250 prep/Kit
  • Usually Shipped in: 1-2 Weeks
GeneJET Whole Blood Genomic DNA Purification Mini Kit, 50 preps, 50 prep

Brand: Fermentas

₹ 14,894.00
  • Brand: Fermentas
  • Catalog No.: K0781
  • Quantity/Unit: 50 preps/Kit
  • Usually Shipped in: 1-2 Weeks
GeneJET Whole Blood Genomic DNA Purification Mini Kit, 250 preps, 250 prep

Brand: Fermentas

₹ 66,582.00
  • Brand: Fermentas
  • Catalog No.: K0782
  • Quantity/Unit: 250 preps/Kit
  • Usually Shipped in: 1-2 Weeks
GeneJET Plant Genomic DNA Purification Kit, 50 preps, 50 prep

Brand: Fermentas

₹ 16,508.00
  • Brand: Fermentas
  • Catalog No.: K0791
  • Quantity/Unit: 50 preps/Kit
  • Usually Shipped in: 1-2 Weeks
GeneJET Plant Genomic DNA Purification Kit, 250 preps, 250 prep

Brand: Fermentas

₹ 70,342.00
  • Brand: Fermentas
  • Catalog No.: K0792
  • Quantity/Unit: 250 preps/Kit
  • Usually Shipped in: 1-2 Weeks
GeneJET FFPE DNA Purification Kit, 50 Preps

Brand: Fermentas

₹ 17,043.00
  • Brand: Fermentas
  • Catalog No.: K0881
  • Quantity/Unit: 50 Preps/Kit
  • Usually Shipped in: 1-2 Weeks
GeneJET FFPE DNA Purification Kit, 250 Preps

Brand: Fermentas

₹ 69,625.00
  • Brand: Fermentas
  • Catalog No.: K0882
  • Quantity/Unit: 250 Preps/Kit
  • Usually Shipped in: 1-2 Weeks
Lysis Solution (for kits K0721/2), 60 mL

Brand: Fermentas

₹ 7,374.00
  • Brand: Fermentas
  • Catalog No.: R1511
  • Quantity/Unit: 60 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Washing Buffer I for kits K0721/2, K0791/2), 40 mL

Brand: Fermentas

₹ 5,066.00
  • Brand: Fermentas
  • Catalog No.: R1521
  • Quantity/Unit: 40 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Washing Buffer II (for kits K0721/2, K0781/2, K0791/2), 40 mL

Brand: Fermentas

₹ 4,255.00
  • Brand: Fermentas
  • Catalog No.: R1531
  • Quantity/Unit: 40 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Elution Buffer (for kits K0721/2, K0781/2, K0791/2), 150 mL

Brand: Fermentas

₹ 5,066.00
  • Brand: Fermentas
  • Catalog No.: R1571
  • Quantity/Unit: 150 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Digestion Buf/GeneJET FFPE, 55 mL

Brand: Fermentas

₹ 21,850.00
  • Brand: Fermentas
  • Catalog No.: R2171
  • Quantity/Unit: 55 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Binding Buf/GeneJET FFPE, 55 mL

Brand: Fermentas

₹ 21,850.00
  • Brand: Fermentas
  • Catalog No.: R2181
  • Quantity/Unit: 55 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Wash Buf1/GeneJET FFPE, 40 mL

Brand: Fermentas

₹ 11,936.00
  • Brand: Fermentas
  • Catalog No.: R2191
  • Quantity/Unit: 40 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Wash Buf2/GeneJET FFPE, 40 mL

Brand: Fermentas

₹ 11,936.00
  • Brand: Fermentas
  • Catalog No.: R2201
  • Quantity/Unit: 40 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
Elution Buf/GeneJET FFPE, 40 mL

Brand: Fermentas

₹ 7,895.00
  • Brand: Fermentas
  • Catalog No.: R2211
  • Quantity/Unit: 40 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
MagJET Rack, 4x50 mL, 1 each

Brand: Fermentas

  • Brand: Fermentas
  • Catalog No.: MR04
  • Quantity/Unit: 1 Rack/Box
  • Usually Shipped in: 1-2 Weeks
MagJET Separation Rack, x96, 1 each

Brand: Fermentas

  • Brand: Fermentas
  • Catalog No.: MR03
  • Quantity/Unit: 1 Rack/Box
  • Usually Shipped in: 1-2 Weeks
GeNei Plant DNA Extraction Kit -2102200051730

Brand: Bangalore GeNei

₹ 6,300.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2102200051730
  • Quantity/Unit: 100 preps/Kit
  • Usually Shipped in: 2-3 Weeks
GeneiPure Quick PCR Purification Kit-2115300031730

Brand: Bangalore GeNei

₹ 10,670.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2115300031730
  • Quantity/Unit: 50 preps/Kit
  • Usually Shipped in: 2-3 Weeks