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DNA Purification and Extraction

Swiss Biologist Friedrich Miescher was the first person to isolate a DNA. The process refers to both extraction and purification of DNA from the given sample such as blood, swab, plant tissue, animal tissue, bacterial cells, etc. A general protocol involves homogenization of cells using a physical method like the use of mortar and pestle or automated homogenizer. This may be followed by a series of chemical treatment with detergents to remove fats, rupture cellular membranes and then by using Chloroform & further centrifugation, DNA can be separated from the cellular debris. A high temperature may be maintained to promote cell lysis. A buffer like Tris to maintain pH is used throughout. Ethanol or isopropanol is then used to precipitate out DNA. Purification may involve many steps like the use of proteinase or phenol-chloroform extraction to eliminate protein impurities or RNAse to remove RNA.
 
Kits for extraction and purification of DNA specific to the source of DNA are available for ready-to-use, or separate components can be used to develop a standardized protocol.
 

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Agarose Gel Extraction Kit- MPK7

Agarose Gel Extraction Kit- MPK7
₹ 2,500.00

Brand: Aura Biotech

Catalog No.: MPK7

Quantity/Unit: 20 Rxns/Pack

Usually Shipped in: 1-3 Days

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Agarose Gel Extraction Kit- MPK8

Agarose Gel Extraction Kit- MPK8
₹ 6,500.00

Brand: Aura Biotech

Catalog No.: MPK8

Quantity/Unit: 50 Rxns/Pack

Usually Shipped in: 1-3 Days

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Agarose Gel Extraction Kit- MPK9

Agarose Gel Extraction Kit- MPK9
₹ 22,500.00

Brand: Aura Biotech

Catalog No.: MPK9

Quantity/Unit: 200 Rxns/Pack

Usually Shipped in: 1-3 Days

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Bacterial DNA Extraction Kit-TBAC-50

Bacterial DNA Extraction Kit-TBAC-50
₹ 5,250.00

Brand: Transiom Genomics

Catalog No.: TBAC-50

Quantity/Unit: 50 Extraction/Box

Usually Shipped in: 4-7 Days

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Binding Buf/GeneJET FFPE, 55 mL

Binding Buf/GeneJET FFPE, 55 mL
₹ 16,902.90

Brand: Fermentas

Catalog No.: R2181

Quantity/Unit: 55 mL/Bottle

Usually Shipped in: 1-2 Weeks

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Binding Buf/MagJET Blood DNA

Binding Buf/MagJET Blood DNA

Brand: Finnzymes

Catalog No.: FERR2352

Quantity/Unit: 90 mL/Bottle

Usually Shipped in: 2-3 Weeks

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Binding Buf/MagJET Blood DNA-FERR2351

Binding Buf/MagJET Blood DNA-FERR2351
₹ 11,740.00

Brand: Finnzymes

Catalog No.: FERR2351

Quantity/Unit: 23 mL/Kit

Usually Shipped in: 2-3 Weeks

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Binding Buff/GeneJET NGS Clean

Binding Buff/GeneJET NGS Clean

Brand: Finnzymes

Catalog No.: FERR2811

Quantity/Unit: 165 mL/Bottle

Usually Shipped in: 2-3 Weeks

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Blood DNA Extraction Kit-TBDK-50

Blood DNA Extraction Kit-TBDK-50
₹ 4,500.00

Brand: Transiom Genomics

Catalog No.: TBDK-50

Quantity/Unit: 50 Extraction/Box

Usually Shipped in: 4-7 Days

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DNA Extraction Kit-Fungal-TFDK-50

DNA Extraction Kit-Fungal-TFDK-50
₹ 5,250.00

Brand: Transiom Genomics

Catalog No.: TFDK-50

Quantity/Unit: 50 Extraction/Box

Usually Shipped in: 4-7 Days

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DNA Extraction Kit-Insect-TIDK-50

DNA Extraction Kit-Insect-TIDK-50
₹ 5,050.00

Brand: Transiom Genomics

Catalog No.: TIDK-50

Quantity/Unit: 50 Extraction/Box

Usually Shipped in: 4-7 Days

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DNA Extraction Kit-Milk-TMDK-50

DNA Extraction Kit-Milk-TMDK-50
₹ 4,850.00

Brand: Transiom Genomics

Catalog No.: TMDK-50

Quantity/Unit: 50 Extraction/Box

Usually Shipped in: 4-7 Days

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DNA Extraction Kit-Plant-TPDK-50

DNA Extraction Kit-Plant-TPDK-50
₹ 4,350.00

Brand: Transiom Genomics

Catalog No.: TPDK-50

Quantity/Unit: 50 Extraction/Box

Usually Shipped in: 4-7 Days

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DNA Extraction Kit-Plasmid-TPLA-50

DNA Extraction Kit-Plasmid-TPLA-50
₹ 3,750.00

Brand: Transiom Genomics

Catalog No.: TPLA-50

Quantity/Unit: 50 Extraction/Box

Usually Shipped in: 4-7 Days

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DNA Extraction Kit-Tissue-TTDK-50

DNA Extraction Kit-Tissue-TTDK-50
₹ 5,700.00

Brand: Transiom Genomics

Catalog No.: TTDK-50

Quantity/Unit: 50 Extraction/Box

Usually Shipped in: 4-7 Days

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