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DNA Purification and Extraction

Swiss Biologist Friedrich Miescher was the first person to isolate a DNA. The process refers to both extraction and purification of DNA from the given sample such as blood, swab, plant tissue, animal tissue, bacterial cells, etc. A general protocol involves homogenization of cells using a physical method like the use of mortar and pestle or automated homogenizer. This may be followed by a series of chemical treatment with detergents to remove fats, rupture cellular membranes and then by using Chloroform & further centrifugation, DNA can be separated from the cellular debris. A high temperature may be maintained to promote cell lysis. A buffer like Tris to maintain pH is used throughout. Ethanol or isopropanol is then used to precipitate out DNA. Purification may involve many steps like the use of proteinase or phenol-chloroform extraction to eliminate protein impurities or RNAse to remove RNA.

Kits for extraction and purification of DNA specific to the source of DNA are available for ready-to-use, or separate components can be used to develop a standardized protocol.


Brand: Finnzymes

Catalog No.: FERR2352

Quantity/Unit: 90 mL/Bottle

Brand: Finnzymes

Catalog No.: FERR2351

Quantity/Unit: 23 mL/Bottle

₹ 22,630.00 22630

Brand: Finnzymes

Catalog No.: FERR2811

Quantity/Unit: 165 mL/Bottle

Brand: Finnzymes

Catalog No.: FERR2701

Quantity/Unit: 210 mL/Bottle

₹ 15,341.00 15341

Brand: Bangalore GeNei

Catalog No.: 610667800021730

Quantity/Unit: 20 preparations/Kit

₹ 3,190.00 3190

Brand: Bangalore GeNei

Catalog No.: 612105500011730

Quantity/Unit: 10 preparations/Kit

Brand: Finnzymes

Catalog No.: FERR2451

Quantity/Unit: 22 mL/Bottle

Brand: Finnzymes

Catalog No.: FERR2452

Quantity/Unit: 90 mL/Bottle

₹ 1,28,489.00 128489

Brand: HiMedia

Catalog No.: MB548-12X96PR

Quantity/Unit: 12X96pr*/EA