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DNA Purification and Extraction

Swiss Biologist Friedrich Miescher was the first person to isolate a DNA. The process refers to both extraction and purification of DNA from the given sample such as blood, swab, plant tissue, animal tissue, bacterial cells, etc. A general protocol involves homogenization of cells using a physical method like the use of mortar and pestle or automated homogenizer. This may be followed by a series of chemical treatment with detergents to remove fats, rupture cellular membranes and then by using Chloroform & further centrifugation, DNA can be separated from the cellular debris. A high temperature may be maintained to promote cell lysis. A buffer like Tris to maintain pH is used throughout. Ethanol or isopropanol is then used to precipitate out DNA. Purification may involve many steps like the use of proteinase or phenol-chloroform extraction to eliminate protein impurities or RNAse to remove RNA.
 
Kits for extraction and purification of DNA specific to the source of DNA are available for ready-to-use, or separate components can be used to develop a standardized protocol.
 

PRODUCTS

GeneJET Plasmid Miniprep Kit, 250 prep

GeneJET Plasmid Miniprep Kit, 250 prep

₹ 22,637.00

Brand: Fermentas

Catalog No.: K0503

Quantity/Unit: 250 prep/Kit

Usually Shipped in: 1-2 Weeks

GeneJET Plasmid Midiprep Kit, 25 prep

GeneJET Plasmid Midiprep Kit, 25 prep

₹ 17,911.00

Brand: Fermentas

Catalog No.: K0481

Quantity/Unit: 25 prep/Kit

Usually Shipped in: 1-2 Weeks

GeneJET Plasmid Midiprep Kit, 100 prep

GeneJET Plasmid Midiprep Kit, 100 prep

₹ 64,409.00

Brand: Fermentas

Catalog No.: K0482

Quantity/Unit: 100 prep/Kit

Usually Shipped in: 1-2 Weeks

GeneJET Plasmid Maxiprep Kit, 10 prep

GeneJET Plasmid Maxiprep Kit, 10 prep

₹ 15,821.00

Brand: Fermentas

Catalog No.: K0491

Quantity/Unit: 10 prep/Kit

Usually Shipped in: 1-2 Weeks

GeneJET Plasmid Maxiprep Kit, 25 prep

GeneJET Plasmid Maxiprep Kit, 25 prep

₹ 32,983.00

Brand: Fermentas

Catalog No.: K0492

Quantity/Unit: 25 prep/Kit

Usually Shipped in: 1-2 Weeks

GeneJET Endo-free Plasmid Maxi Kit, 10 Preps

GeneJET Endo-free Plasmid Maxi Kit, 10 Preps

Brand: Fermentas

Catalog No.: K0861

Quantity/Unit: 10 Prep/Kit

Usually Shipped in: 1-2 Weeks

Genomic DNA Purification Kit, 100 prep

Genomic DNA Purification Kit, 100 prep

₹ 12,201.00

Brand: Fermentas

Catalog No.: K0512

Quantity/Unit: 100 prep/Kit

Usually Shipped in: 1-2 Weeks

GeneJET Genomic DNA Purification Kit, 50 prep

GeneJET Genomic DNA Purification Kit, 50 prep

₹ 18,308.00

Brand: Fermentas

Catalog No.: K0721

Quantity/Unit: 50 prep/Kit

Usually Shipped in: 1-2 Weeks

GeneJET Genomic DNA Purification Kit, 250 prep

GeneJET Genomic DNA Purification Kit, 250 prep

₹ 82,959.00

Brand: Fermentas

Catalog No.: K0722

Quantity/Unit: 250 prep/Kit

Usually Shipped in: 1-2 Weeks

GeneJET Whole Blood Genomic DNA Purification Mini Kit, 50 preps, 50 prep

GeneJET Whole Blood Genomic DNA Purification Mini Kit, 50 preps, 50 prep

₹ 13,019.00

Brand: Fermentas

Catalog No.: K0781

Quantity/Unit: 50 preps/Kit

Usually Shipped in: 1-2 Weeks

GeneJET Whole Blood Genomic DNA Purification Mini Kit, 250 preps, 250 prep

GeneJET Whole Blood Genomic DNA Purification Mini Kit, 250 preps, 250 prep

₹ 58,201.00

Brand: Fermentas

Catalog No.: K0782

Quantity/Unit: 250 preps/Kit

Usually Shipped in: 1-2 Weeks

GeneJET Plant Genomic DNA Purification Kit, 50 preps, 50 prep

GeneJET Plant Genomic DNA Purification Kit, 50 preps, 50 prep

₹ 14,430.00

Brand: Fermentas

Catalog No.: K0791

Quantity/Unit: 50 preps/Kit

Usually Shipped in: 1-2 Weeks

GeneJET Plant Genomic DNA Purification Kit, 250 preps, 250 prep

GeneJET Plant Genomic DNA Purification Kit, 250 preps, 250 prep

₹ 61,488.00

Brand: Fermentas

Catalog No.: K0792

Quantity/Unit: 250 preps/Kit

Usually Shipped in: 1-2 Weeks

GeneJET FFPE DNA Purification Kit, 50 Preps

GeneJET FFPE DNA Purification Kit, 50 Preps

₹ 14,756.00

Brand: Fermentas

Catalog No.: K0881

Quantity/Unit: 50 Preps/Kit

Usually Shipped in: 1-2 Weeks

GeneJET FFPE DNA Purification Kit, 250 Preps

GeneJET FFPE DNA Purification Kit, 250 Preps

₹ 60,860.00

Brand: Fermentas

Catalog No.: K0882

Quantity/Unit: 250 Preps/Kit

Usually Shipped in: 1-2 Weeks