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DNA Purification and Extraction

DNA Purification and Extraction

Swiss Biologist Friedrich Miescher was the first person to isolate a DNA. The process refers to both extraction and purification of DNA from the given sample such as blood, swab, plant tissue, animal tissue, bacterial cells, etc. A general protocol involves homogenization of cells using a physical method like the use of mortar and pestle or automated homogenizer. This may be followed by a series of chemical treatment with detergents to remove fats, rupture cellular membranes and then by using Chloroform & further centrifugation, DNA can be separated from the cellular debris. A high temperature may be maintained to promote cell lysis. A buffer like Tris to maintain pH is used throughout. Ethanol or isopropanol is then used to precipitate out DNA. Purification may involve many steps like the use of proteinase or phenol-chloroform extraction to eliminate protein impurities or RNAse to remove RNA.
 
Kits for extraction and purification of DNA specific to the source of DNA are available for ready-to-use, or separate components can be used to develop a standardized protocol.
 
CamSpin Flex Columns

Brand: Cambrian Bioworks

₹ 3,250.00
  • Brand: Cambrian Bioworks
  • Catalog No.: CBWB009
  • Quantity/Unit: 500 units/Box
  • Availability: In stock
CamSpin Pure Columns

Brand: Cambrian Bioworks

₹ 3,250.00
  • Brand: Cambrian Bioworks
  • Catalog No.: CBWB010
  • Quantity/Unit: 500 units/Box
  • Availability: In stock
SpinTech HiQ DNA Extraction Kit For Blood, 10 Reactions

Brand: SpinTech HIQ

₹ 2,500.00
  • Brand: SpinTech HIQ
  • Catalog No.: TLPL-007
  • Quantity/Unit: 10 Reactions/Pack
  • Usually Shipped in: 1-2 Weeks
SpinTech HiQ DNA Extraction Kit For Blood, 25 Reactions

Brand: SpinTech HIQ

₹ 4,000.00
  • Brand: SpinTech HIQ
  • Catalog No.: TLPL-008
  • Quantity/Unit: 25 Reactions/Pack
  • Usually Shipped in: 1-2 Weeks
SpinTech HiQ DNA Extraction Kit For Blood, 50 Reactions

Brand: SpinTech HIQ

₹ 7,500.00
  • Brand: SpinTech HIQ
  • Catalog No.: TLPL-009
  • Quantity/Unit: 50 Reactions/Pack
  • Usually Shipped in: 1-2 Weeks
SpinTech HiQ Miniprep Plasmid Extraction Kit, 10 Reactions

Brand: SpinTech HIQ

₹ 2,500.00
  • Brand: SpinTech HIQ
  • Catalog No.: TLPL-010
  • Quantity/Unit: 10 Reactions/Pack
  • Usually Shipped in: 1-2 Weeks
SpinTech HiQ Miniprep Plasmid Extraction Kit, 25 Reactions

Brand: SpinTech HIQ

₹ 5,000.00
  • Brand: SpinTech HIQ
  • Catalog No.: TLPL-011
  • Quantity/Unit: 25 Reactions/Pack
  • Usually Shipped in: 1-2 Weeks
SpinTech HiQ Miniprep Plasmid Extraction Kit, 50 Reactions

Brand: SpinTech HIQ

₹ 10,000.00
  • Brand: SpinTech HIQ
  • Catalog No.: TLPL-012
  • Quantity/Unit: 50 Reactions/Pack
  • Usually Shipped in: 1-2 Weeks
SpinTech HiQ Gel Extraction Kit, 10 Reactions

Brand: SpinTech HIQ

₹ 2,500.00
  • Brand: SpinTech HIQ
  • Catalog No.: TLPL-013
  • Quantity/Unit: 10 Reactions/Pack
  • Usually Shipped in: 1-2 Weeks
SpinTech HiQ Gel Extraction Kit, 50 Reactions

Brand: SpinTech HIQ

₹ 10,000.00
  • Brand: SpinTech HIQ
  • Catalog No.: TLPL-015
  • Quantity/Unit: 50 Reactions/Pack
  • Usually Shipped in: 1-2 Weeks
SpinTech HiQ Gel Extraction Kit, 25 Reactions

Brand: SpinTech HIQ

₹ 5,000.00
  • Brand: SpinTech HIQ
  • Catalog No.: TLPL-014
  • Quantity/Unit: 25 Reactions/Pack
  • Usually Shipped in: 1-2 Weeks
FB Plasmid Miniprep Kit-100 rxn

Brand: BR Biochem Life Sciences

₹ 9,492.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BSP0100
  • Quantity/Unit: 100 rxn/Kit
  • Usually Shipped in: 3-4 Weeks
FB Plasmid Miniprep Kit-300 rxn

Brand: BR Biochem Life Sciences

₹ 25,425.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BSP0300
  • Quantity/Unit: 300 rxn/Kit
  • Usually Shipped in: 3-4 Weeks
FB Plasmid Maxiprep Kit-10 rxn

Brand: BR Biochem Life Sciences

₹ 19,662.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BXP010
  • Quantity/Unit: 10 rxn/Kit
  • Usually Shipped in: 3-4 Weeks
FB Plasmid Maxiprep Kit-20 rxn

Brand: BR Biochem Life Sciences

₹ 37,629.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BXP020
  • Quantity/Unit: 20 rxn/Kit
  • Usually Shipped in: 3-4 Weeks
Plasmid DNA Isolation Kit- MPK3

Brand: Aura Biotech

₹ 15,000.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-171L
  • Quantity/Unit: 200 Rxns/Pack
  • Usually Shipped in: 4-7 Days
Agarose Gel Extraction Kit- MPK7

Brand: Aura Biotech

₹ 3,000.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-173S
  • Quantity/Unit: 20 Rxns/Pack
  • Usually Shipped in: 4-7 Days
Agarose Gel Extraction Kit- MPK8

Brand: Aura Biotech

₹ 7,500.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-173M
  • Quantity/Unit: 50 Rxns/Pack
  • Usually Shipped in: 4-7 Days
Agarose Gel Extraction Kit- MPK9

Brand: Aura Biotech

₹ 25,500.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-173XL
  • Quantity/Unit: 250 Rxns/Pack
  • Usually Shipped in: 4-7 Days
Aura Pure sputum DNA Isolation Kit, 250 Rxns

Brand: Aura Biotech

₹ 35,750.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-134L
  • Quantity/Unit: 250 Reactions/Pack
  • Availability: In stock
Aura Pure Bacterial Genomic DNA Isolation Kit, 50 Rxns

Brand: Aura Biotech

₹ 7,500.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-135S
  • Quantity/Unit: 50 Reactions/Pack
  • Availability: In stock
Aura Pure Bacterial Genomic DNA Isolation Kit, 100 Rxns

Brand: Aura Biotech

₹ 11,500.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-135M
  • Quantity/Unit: 100 Reactions/Pack
  • Availability: In stock
Aura Pure Bacterial Genomic DNA Isolation Kit, 250 Rxns

Brand: Aura Biotech

₹ 20,750.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-135L
  • Quantity/Unit: 250 Reactions/Pack
  • Availability: In stock
Aura Pure Fungal DNA Isolation Kit, 50 Rxns

Brand: Aura Biotech

₹ 9,500.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-136S
  • Quantity/Unit: 50 Reactions/Pack
  • Availability: In stock
Aura Pure Fungal DNA Isolation Kit, 100 Rxns

Brand: Aura Biotech

₹ 16,000.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-136M
  • Quantity/Unit: 100 Reactions/Pack
  • Availability: In stock
Aura Pure Fungal DNA Isolation Kit, 250 Rxns

Brand: Aura Biotech

₹ 31,000.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-136L
  • Quantity/Unit: 250 Reactions/Pack
  • Availability: In stock
Aura Pure Milk DNA Isolation Kit, 50 Rxns

Brand: Aura Biotech

₹ 9,500.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-137S
  • Quantity/Unit: 50 Reactions/Pack
  • Availability: In stock
Aura Pure Milk DNA Isolation Kit, 100 Rxns

Brand: Aura Biotech

₹ 16,000.00
  • Brand: Aura Biotech
  • Catalog No.: ABT-137M
  • Quantity/Unit: 100 Reactions/Pack
  • Availability: In stock