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DNA Purification and Extraction

DNA Purification and Extraction

Swiss Biologist Friedrich Miescher was the first person to isolate a DNA. The process refers to both extraction and purification of DNA from the given sample such as blood, swab, plant tissue, animal tissue, bacterial cells, etc. A general protocol involves homogenization of cells using a physical method like the use of mortar and pestle or automated homogenizer. This may be followed by a series of chemical treatment with detergents to remove fats, rupture cellular membranes and then by using Chloroform & further centrifugation, DNA can be separated from the cellular debris. A high temperature may be maintained to promote cell lysis. A buffer like Tris to maintain pH is used throughout. Ethanol or isopropanol is then used to precipitate out DNA. Purification may involve many steps like the use of proteinase or phenol-chloroform extraction to eliminate protein impurities or RNAse to remove RNA.
 
Kits for extraction and purification of DNA specific to the source of DNA are available for ready-to-use, or separate components can be used to develop a standardized protocol.
 
EXTRACTME DNA CLEAN-UP KIT, 250 preps
₹ 18,653.25
  • Brand: Blirt
  • Catalog No.: EM07.1-250
  • Quantity/Unit: 250 preps/pack
Insta NX Frozen Blood DNA Purification Kit-MBIN017-12PR
₹ 6,175.00
  • Brand: HIMEDIA
  • Catalog No.: MBIN017-12PR
  • Quantity/Unit: 12PR/Pack
  • Usually Shipped in: 2-3 Weeks
Insta NX Food Pathogen DNA Purification Kit-MBIN018-12PR
₹ 6,175.00
  • Brand: HIMEDIA
  • Catalog No.: MBIN018-12PR
  • Quantity/Unit: 12PR/Pack
  • Usually Shipped in: 2-3 Weeks
HiPurA Urine DNA Purification Kit-MB573-20PR
₹ 2,740.00
  • Brand: HIMEDIA
  • Catalog No.: MB573-20PR
  • Quantity/Unit: 20PR/Pack
  • Usually Shipped in: 2-3 Weeks
HiPurA Urine DNA Purification Kit-MB573-250PR
₹ 26,840.00
  • Brand: HIMEDIA
  • Catalog No.: MB573-250PR
  • Quantity/Unit: 250PR/Pack
  • Usually Shipped in: 2-3 Weeks
HiPurA Viral DNA Purification Kit-MB575-20PR
₹ 5,230.00
  • Brand: HIMEDIA
  • Catalog No.: MB575-20PR
  • Quantity/Unit: 20PR/Pack
  • Usually Shipped in: 2-3 Weeks
EXTRACTME PLASMID MINI DNA KIT, 250 preps
₹ 19,099.50
  • Brand: Blirt
  • Catalog No.: EM01.1-250
  • Quantity/Unit: 250 preps/pack
EXTRACTME PLASMID MIDI KIT, 10 preps
₹ 8,746.50
  • Brand: Blirt
  • Catalog No.: EM16-010
  • Quantity/Unit: 10 preps/pack
EXTRACTME DNA CLEAN-UP KIT, 50 preps
₹ 4,551.75
  • Brand: Blirt
  • Catalog No.: EM07.1-050
  • Quantity/Unit: 50 preps/pack
FB Plasmid Miniprep Kit-300 rxn
₹ 25,425.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BSP0300
  • Quantity/Unit: 300 rxn/Kit
  • Usually Shipped in: 3-4 Weeks
DNA Extraction Kit-Fungal-TFDK-50
₹ 6,825.00
  • Brand: Transiom Genomics
  • Catalog No.: TFDK-50
  • Quantity/Unit: 50 Extraction/Box
Genomic DNA Purification Kit, 100 prep
₹ 12,201.00
  • Brand: Fermentas
  • Catalog No.: K0512
  • Quantity/Unit: 100 prep/Kit
  • Usually Shipped in: 1-2 Weeks
EXTRACTME DNA CLEAN-UP & GEL-OUT KIT, 50 preps
₹ 5,355.00
  • Brand: Blirt
  • Catalog No.: EM26.1-050
  • Quantity/Unit: 50 preps/pack
HiPurA Yeast Genomic DNA Purification Kit-MB552-50PR
₹ 7,885.00
  • Brand: HIMEDIA
  • Catalog No.: MB552-50PR
  • Quantity/Unit: 50pr*/EA
  • Usually Shipped in: 2-3 Weeks
HiPurA Food DNA Purification Kit-MB562-50PR
₹ 8,030.00
  • Brand: HIMEDIA
  • Catalog No.: MB562-50PR
  • Quantity/Unit: 50pr*/EA
  • Usually Shipped in: 2-3 Weeks
HiPurA Water DNA Purification Kit-MB577-250PR
₹ 34,085.00
  • Brand: HIMEDIA
  • Catalog No.: MB577-250PR
  • Quantity/Unit: 250PR/Pack
  • Usually Shipped in: 2-3 Weeks
HiPurA Multi-Sample DNA Purification Kit-MB554-20PR
₹ 3,790.00
  • Brand: HIMEDIA
  • Catalog No.: MB554-20PR
  • Quantity/Unit: 20pr*/EA
  • Usually Shipped in: 2-3 Weeks
GeneiPure Plant Genomic DNA Purification Kit-2115700021730
₹ 10,054.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2115700021730
  • Quantity/Unit: 20 preps/Kit
Wash Buf2/GeneJET FFPE, 40 mL
₹ 10,434.00
  • Brand: Fermentas
  • Catalog No.: R2201
  • Quantity/Unit: 40 mL/Bottle
  • Usually Shipped in: 1-2 Weeks
GeneiPure Fungal DNA Purification Kit
₹ 9,108.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2117300021730
  • Quantity/Unit: 1 Ea/Pack
EXTRACTME GENOMIC DNA KIT - universal, 50 preps
₹ 9,996.00
  • Brand: Blirt
  • Catalog No.: EM13-050
  • Quantity/Unit: 50 preps/pack
Geneasy Urine/Body Fluid swabs DNA lsolation Kit-UDIK 50
₹ 3,440.00 ₹ 4,300.00
20% Off
  • Brand: BHAT BIO-TECH INDIA PVT. LTD.
  • Catalog No.: UDIK 50
  • Quantity/Unit: 50 Reactions/Pack
  • Usually Shipped in: 4-7 Days
GeneiPure Mammalian Genomic DNA Purification Kit-2115500031730
₹ 16,577.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2115500031730
  • Quantity/Unit: 50 preps/Kit
GeneJET Plasmid Miniprep Kit, 250 prep
₹ 22,637.00
  • Brand: Fermentas
  • Catalog No.: K0503
  • Quantity/Unit: 250 prep/Kit
  • Usually Shipped in: 1-2 Weeks
HiPurA Banana DNA Purification Kit
₹ 39,750.00
  • Brand: HIMEDIA
  • Catalog No.: MB564-250PR
  • Quantity/Unit: 250pr*/EA
  • Usually Shipped in: 2-3 Weeks
HiPer Yeast Genomic DNA Extraction Teaching Kit-HTBM013-10PR
₹ 3,955.00
  • Brand: HIMEDIA
  • Catalog No.: HTBM013-10PR
  • Quantity/Unit: 10PR/Pack
  • Usually Shipped in: 2-3 Weeks
Plasmid DNA Extraction Teaching Kit (Solution Based)
₹ 2,700.00
  • Brand: Aura Biotech
  • Catalog No.: MPL-05
  • Quantity/Unit: 10 Rxns/Pack
  • Usually Shipped in: 1-3 Days
Genomic DNA Isolation Teaching Kit
₹ 3,200.00
  • Brand: Aura Biotech
  • Catalog No.: MPL-13
  • Quantity/Unit: 10 Rxns/Pack
  • Usually Shipped in: 1-3 Days