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DNA Purification and Extraction

DNA Purification and Extraction

Swiss Biologist Friedrich Miescher was the first person to isolate a DNA. The process refers to both extraction and purification of DNA from the given sample such as blood, swab, plant tissue, animal tissue, bacterial cells, etc. A general protocol involves homogenization of cells using a physical method like the use of mortar and pestle or automated homogenizer. This may be followed by a series of chemical treatment with detergents to remove fats, rupture cellular membranes and then by using Chloroform & further centrifugation, DNA can be separated from the cellular debris. A high temperature may be maintained to promote cell lysis. A buffer like Tris to maintain pH is used throughout. Ethanol or isopropanol is then used to precipitate out DNA. Purification may involve many steps like the use of proteinase or phenol-chloroform extraction to eliminate protein impurities or RNAse to remove RNA.
 
Kits for extraction and purification of DNA specific to the source of DNA are available for ready-to-use, or separate components can be used to develop a standardized protocol.
 
Geneasy Whole Blood/Mammalian Cells/Tissues DNA lsolation Kit-WBDIK 25

Brand: BHAT BIO-TECH INDIA PVT. LTD.

₹ 2,300.00
  • Brand: BHAT BIO-TECH INDIA PVT. LTD.
  • Catalog No.: WBDIK 25
  • Quantity/Unit: 25 Reactions/Pack
  • Usually Shipped in: 4-7 Days
FB Plasmid Maxiprep Kit-10 rxn

Brand: BR Biochem Life Sciences

₹ 19,662.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BXP010
  • Quantity/Unit: 10 rxn/Kit
  • Usually Shipped in: 3-4 Weeks
Geneasy Plant DNA lsolation Kit-PLDIK 50

Brand: BHAT BIO-TECH INDIA PVT. LTD.

₹ 5,200.00
  • Brand: BHAT BIO-TECH INDIA PVT. LTD.
  • Catalog No.: PLDIK 50
  • Quantity/Unit: 50 Reactions/Pack
  • Usually Shipped in: 4-7 Days
Gel Extraction Teaching Kit

Brand: Aura Biotech

₹ 3,400.00
  • Brand: Aura Biotech
  • Catalog No.: MPL-06
  • Quantity/Unit: 10 Rxns/Pack
  • Usually Shipped in: 1-3 Days
Geneasy Fungal DNA lsolation Kit-FDIK 25

Brand: BHAT BIO-TECH INDIA PVT. LTD.

₹ 3,400.00
  • Brand: BHAT BIO-TECH INDIA PVT. LTD.
  • Catalog No.: FDIK 25
  • Quantity/Unit: 25 Reactions/Pack
  • Usually Shipped in: 4-7 Days
Geneasy Plant DNA lsolation Kit-PLDIK 25

Brand: BHAT BIO-TECH INDIA PVT. LTD.

₹ 2,160.00 ₹ 2,700.00
20% Off
  • Brand: BHAT BIO-TECH INDIA PVT. LTD.
  • Catalog No.: PLDIK 25
  • Quantity/Unit: 25 Reactions/Pack
  • Usually Shipped in: 4-7 Days
Geneasy Whole Blood/Mammalian Cells/Tissues DNA lsolation Kit-WBDIK 50

Brand: BHAT BIO-TECH INDIA PVT. LTD.

₹ 4,100.00
  • Brand: BHAT BIO-TECH INDIA PVT. LTD.
  • Catalog No.: WBDIK 50
  • Quantity/Unit: 50 Reactions/Pack
  • Usually Shipped in: 4-7 Days
Geneasy DNA Gel Extraction Kit-DGEK 25

Brand: BHAT BIO-TECH INDIA PVT. LTD.

₹ 3,200.00
  • Brand: BHAT BIO-TECH INDIA PVT. LTD.
  • Catalog No.: DGEK 25
  • Quantity/Unit: 25 Reactions/Pack
  • Usually Shipped in: 4-7 Days
Geneasy PCR Product Purification Kit-PPPK 50

Brand: BHAT BIO-TECH INDIA PVT. LTD.

₹ 6,300.00
  • Brand: BHAT BIO-TECH INDIA PVT. LTD.
  • Catalog No.: PPPK 50
  • Quantity/Unit: 50 Reactions/Pack
  • Usually Shipped in: 4-7 Days
Geneasy PCR Product Purification Kit-PPPK 25

Brand: BHAT BIO-TECH INDIA PVT. LTD.

₹ 3,500.00
  • Brand: BHAT BIO-TECH INDIA PVT. LTD.
  • Catalog No.: PPPK 25
  • Quantity/Unit: 25 Reactions/Pack
  • Usually Shipped in: 4-7 Days
Plasmid DNA Isolation Kit- MPK1

Brand: Aura Biotech

₹ 1,700.00
  • Brand: Aura Biotech
  • Catalog No.: MPK1
  • Quantity/Unit: 20 Rxns/Pack
  • Usually Shipped in: 1-3 Days
FB Plasmid Miniprep Kit-100 rxn

Brand: BR Biochem Life Sciences

₹ 9,492.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BSP0100
  • Quantity/Unit: 100 rxn/Kit
  • Usually Shipped in: 3-4 Weeks
FB Plasmid Miniprep Kit-300 rxn

Brand: BR Biochem Life Sciences

₹ 25,425.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BSP0300
  • Quantity/Unit: 300 rxn/Kit
  • Usually Shipped in: 3-4 Weeks
Plasmid DNA Extraction Teaching Kit (Coloum Based)

Brand: Aura Biotech

₹ 2,700.00
  • Brand: Aura Biotech
  • Catalog No.: MPL-04
  • Quantity/Unit: 10 Rxns/Pack
  • Usually Shipped in: 1-3 Days
Geneasy Fungal DNA lsolation Kit-FDIK 50

Brand: BHAT BIO-TECH INDIA PVT. LTD.

₹ 4,880.00 ₹ 6,100.00
20% Off
  • Brand: BHAT BIO-TECH INDIA PVT. LTD.
  • Catalog No.: FDIK 50
  • Quantity/Unit: 50 Reactions/Pack
  • Usually Shipped in: 4-7 Days
Agarose Gel Extraction Kit- MPK7

Brand: Aura Biotech

₹ 2,500.00
  • Brand: Aura Biotech
  • Catalog No.: MPK7
  • Quantity/Unit: 20 Rxns/Pack
  • Usually Shipped in: 1-3 Days
FB Plasmid Maxiprep Kit-20 rxn

Brand: BR Biochem Life Sciences

₹ 37,629.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BXP020
  • Quantity/Unit: 20 rxn/Kit
  • Usually Shipped in: 3-4 Weeks
Plasmid DNA Extraction Teaching Kit (Solution Based)

Brand: Aura Biotech

₹ 2,700.00
  • Brand: Aura Biotech
  • Catalog No.: MPL-05
  • Quantity/Unit: 10 Rxns/Pack
  • Usually Shipped in: 1-3 Days
Genomic DNA Isolation Teaching Kit

Brand: Aura Biotech

₹ 3,200.00
  • Brand: Aura Biotech
  • Catalog No.: MPL-13
  • Quantity/Unit: 10 Rxns/Pack
  • Usually Shipped in: 1-3 Days
Plasmid DNA Isolation Kit- MPK2

Brand: Aura Biotech

₹ 3,500.00
  • Brand: Aura Biotech
  • Catalog No.: MPK2
  • Quantity/Unit: 50 Rxns/Pack
  • Usually Shipped in: 1-3 Days
Plasmid DNA Isolation Kit- MPK3

Brand: Aura Biotech

₹ 15,000.00
  • Brand: Aura Biotech
  • Catalog No.: MPK3
  • Quantity/Unit: 200 Rxns/Pack
  • Usually Shipped in: 1-3 Days
Agarose Gel Extraction Kit- MPK8

Brand: Aura Biotech

₹ 6,500.00
  • Brand: Aura Biotech
  • Catalog No.: MPK8
  • Quantity/Unit: 50 Rxns/Pack
  • Usually Shipped in: 1-3 Days
DNA Molecular size determination kit

Brand: Aura Biotech

₹ 2,900.00
  • Brand: Aura Biotech
  • Catalog No.: MPL-08
  • Quantity/Unit: 10 Rxns/Pack
  • Usually Shipped in: 1-3 Days
Agarose Gel Extraction Kit- MPK9

Brand: Aura Biotech

₹ 22,500.00
  • Brand: Aura Biotech
  • Catalog No.: MPK9
  • Quantity/Unit: 200 Rxns/Pack
  • Usually Shipped in: 1-3 Days
Geneasy Bacterial DNA lsolation Kit-BDIK 25

Brand: BHAT BIO-TECH INDIA PVT. LTD.

₹ 2,700.00
  • Brand: BHAT BIO-TECH INDIA PVT. LTD.
  • Catalog No.: BDIK 25
  • Quantity/Unit: 25 Reactions/Pack
  • Usually Shipped in: 4-7 Days
Geneasy Plasmid DNA lsolation Kit-PDIK 25

Brand: BHAT BIO-TECH INDIA PVT. LTD.

₹ 1,200.00 ₹ 1,500.00
20% Off
  • Brand: BHAT BIO-TECH INDIA PVT. LTD.
  • Catalog No.: PDIK 25
  • Quantity/Unit: 25 Reactions/Pack
  • Usually Shipped in: 4-7 Days
DNA Extraction Kit-Plasmid-TPLA-50

Brand: Transiom Genomics

₹ 4,875.00
  • Brand: Transiom Genomics
  • Catalog No.: TPLA-50
  • Quantity/Unit: 50 Extraction/Box
  • Usually Shipped in: 4-7 Days
DNA Extraction Kit-Insect-TIDK-50

Brand: Transiom Genomics

₹ 6,565.00
  • Brand: Transiom Genomics
  • Catalog No.: TIDK-50
  • Quantity/Unit: 50 Extraction/Box
  • Usually Shipped in: 4-7 Days