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DNA Purification and Extraction

DNA Purification and Extraction

Swiss Biologist Friedrich Miescher was the first person to isolate a DNA. The process refers to both extraction and purification of DNA from the given sample such as blood, swab, plant tissue, animal tissue, bacterial cells, etc. A general protocol involves homogenization of cells using a physical method like the use of mortar and pestle or automated homogenizer. This may be followed by a series of chemical treatment with detergents to remove fats, rupture cellular membranes and then by using Chloroform & further centrifugation, DNA can be separated from the cellular debris. A high temperature may be maintained to promote cell lysis. A buffer like Tris to maintain pH is used throughout. Ethanol or isopropanol is then used to precipitate out DNA. Purification may involve many steps like the use of proteinase or phenol-chloroform extraction to eliminate protein impurities or RNAse to remove RNA.
 
Kits for extraction and purification of DNA specific to the source of DNA are available for ready-to-use, or separate components can be used to develop a standardized protocol.
 
Geneasy Whole Blood/Mammalian Cells/Tissues DNA lsolation Kit-WBDIK 25
₹ 2,300.00
  • Brand: BHAT BIO-TECH INDIA PVT. LTD.
  • Catalog No.: WBDIK 25
  • Quantity/Unit: 25 Reactions/Pack
  • Usually Shipped in: 4-7 Days
EXTRACTME DNA CLEAN-UP & GEL-OUT KIT, 250 preps
₹ 20,884.50
  • Brand: Blirt
  • Catalog No.: EM26.1-250
  • Quantity/Unit: 250 preps/pack
GeneiPureID DNA Isolation Kit -Dried Blood, 50 preps
₹ 11,484.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2117900031730
  • Quantity/Unit: 50 rxns/pack
GeNei Gel Extraction Teaching Kit-6104300011730
₹ 7,865.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6104300011730
  • Quantity/Unit: 5 expts./Kit
GeNei Plant DNA Extraction Kit -2102200051730
₹ 6,303.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2102200051730
  • Quantity/Unit: 100 preps/Kit
Genomic DNA Purification teaching kit from Bacteria ( DNA binding membrane)
₹ 9,493.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6112800011730
  • Quantity/Unit: 1 Ea/Pack
FB Plasmid Maxiprep Kit-10 rxn
₹ 19,662.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BXP010
  • Quantity/Unit: 10 rxn/Kit
  • Usually Shipped in: 3-4 Weeks
EXTRACTME PLASMID MAXI KIT, 25 preps
₹ 40,608.75
  • Brand: Blirt
  • Catalog No.: EM18-025
  • Quantity/Unit: 25 preps/pack
Geneasy Plant DNA lsolation Kit-PLDIK 50
₹ 5,200.00
  • Brand: BHAT BIO-TECH INDIA PVT. LTD.
  • Catalog No.: PLDIK 50
  • Quantity/Unit: 50 Reactions/Pack
  • Usually Shipped in: 4-7 Days
GeneiPureID DNA Isolation Kit-Semen
₹ 3,619.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2118000021730
  • Quantity/Unit: 10 Rxns/Pack
GeneiPure Plant Genomic DNA Purification Kit-2115700021730
₹ 10,054.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2115700021730
  • Quantity/Unit: 20 preps/Kit
GeneiPure Fungal DNA Purification Kit
₹ 9,108.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2117300021730
  • Quantity/Unit: 1 Ea/Pack
EXTRACTME GENOMIC DNA KIT - universal, 50 preps
₹ 9,996.00
  • Brand: Blirt
  • Catalog No.: EM13-050
  • Quantity/Unit: 50 preps/pack
Geneasy Urine/Body Fluid swabs DNA lsolation Kit-UDIK 50
₹ 3,440.00 ₹ 4,300.00
20% Off
  • Brand: BHAT BIO-TECH INDIA PVT. LTD.
  • Catalog No.: UDIK 50
  • Quantity/Unit: 50 Reactions/Pack
  • Usually Shipped in: 4-7 Days
GeneiPure Mammalian Genomic DNA Purification Kit-2115500031730
₹ 16,577.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2115500031730
  • Quantity/Unit: 50 preps/Kit
GeneiPure Gel Extraction Kit-2115400021730
₹ 6,820.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2115400021730
  • Quantity/Unit: 20 preps/Kit
GeNei CTAB Plant DNA Extraction Kit -2105500011730
₹ 4,037.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2105500011730
  • Quantity/Unit: 10 preps/Kit
GeneiPure Blood Genomic DNA Purification Kit-2115600031730
₹ 14,850.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2115600031730
  • Quantity/Unit: 50 preps/Kit
GeneiPure Bacterial DNA Purification Kit-2115900021730
₹ 8,844.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2115900021730
  • Quantity/Unit: 20 preps/Kit
GeneiPureID DNA Isolation Kit-Bone, 50 preps
₹ 15,818.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2118100031730
  • Quantity/Unit: 50 rxns/pack
GeneiPureID DNA Isolation Kit-Bone, 10 preps
₹ 3,619.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2118100021730
  • Quantity/Unit: 10 Rxns/Pack
Gel Extraction Teaching Kit
₹ 3,400.00
  • Brand: Aura Biotech
  • Catalog No.: MPL-06
  • Quantity/Unit: 10 Rxns/Pack
  • Usually Shipped in: 1-3 Days
GeneiPure Quick PCR Purification Kit-2115300051730
₹ 36,663.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2115300051730
  • Quantity/Unit: 250 preps/Kit
GeneiPure Yeast DNA Preparation Kit-2115800021730
₹ 8,844.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2115800021730
  • Quantity/Unit: 20 preps/Kit
GeneiPureID DNA Isolation Kit-Skin, 10 preps
₹ 3,696.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2121000011730
  • Quantity/Unit: 10 Rxns/Pack
GeneiPureID DNA Isolation Kit-Saliva, 10 preps
₹ 3,762.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2118400021730
  • Quantity/Unit: 10 Rxns/Pack
GeNei Plant Mitochondrial DNA Isolation Teaching Kit-6114800011730
₹ 8,514.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6114800011730
  • Quantity/Unit: 5 expts./Kit
GeNei Plasmid DNA Purification Teaching Kit-6112600011730
₹ 5,874.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6112600011730
  • Quantity/Unit: 10 expts./Kit