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DNA Purification and Extraction

Swiss Biologist Friedrich Miescher was the first person to isolate a DNA. The process refers to both extraction and purification of DNA from the given sample such as blood, swab, plant tissue, animal tissue, bacterial cells, etc. A general protocol involves homogenization of cells using a physical method like the use of mortar and pestle or automated homogenizer. This may be followed by a series of chemical treatment with detergents to remove fats, rupture cellular membranes and then by using Chloroform & further centrifugation, DNA can be separated from the cellular debris. A high temperature may be maintained to promote cell lysis. A buffer like Tris to maintain pH is used throughout. Ethanol or isopropanol is then used to precipitate out DNA. Purification may involve many steps like the use of proteinase or phenol-chloroform extraction to eliminate protein impurities or RNAse to remove RNA.
 
Kits for extraction and purification of DNA specific to the source of DNA are available for ready-to-use, or separate components can be used to develop a standardized protocol.
 

PRODUCTS

HiPurASPP Blood DNA Isolation Kit-MB541-10ML

HiPurASPP Blood DNA Isolation Kit-MB541-10ML

₹ 2,273.00

Brand: HIMEDIA

Catalog No.: MB541-10ML

Quantity/Unit: 10ml/EA

Usually Shipped in: 2-3 Weeks

HiPurASPP Blood DNA Isolation Kit-MB541-50ML

HiPurASPP Blood DNA Isolation Kit-MB541-50ML

₹ 5,744.00

Brand: HIMEDIA

Catalog No.: MB541-50ML

Quantity/Unit: 50ml/EA

Usually Shipped in: 2-3 Weeks

HiPurASPP Blood DNA Isolation Kit-MB541-150ML

HiPurASPP Blood DNA Isolation Kit-MB541-150ML

₹ 12,960.00

Brand: HIMEDIA

Catalog No.: MB541-150ML

Quantity/Unit: 150ml/EA

Usually Shipped in: 2-3 Weeks

HiPurASPP Blood DNA Isolation Kit

HiPurASPP Blood DNA Isolation Kit

₹ 20,274.00

Brand: HIMEDIA

Catalog No.: MB541-300ML

Quantity/Unit: 300ml/EA

Usually Shipped in: 2-3 Weeks

HiPurA Blood Genomic DNA Miniprep Purification Kit-MB504-20PR

HiPurA Blood Genomic DNA Miniprep Purification Kit-MB504-20PR

₹ 2,861.00

Brand: HIMEDIA

Catalog No.: MB504-20PR

Quantity/Unit: 20pr*/EA

Usually Shipped in: 2-3 Weeks

HiPurA Blood Genomic DNA Miniprep Purification Kit-MB504-50PR

HiPurA Blood Genomic DNA Miniprep Purification Kit-MB504-50PR

₹ 6,823.00

Brand: HIMEDIA

Catalog No.: MB504-50PR

Quantity/Unit: 50pr*/EA

Usually Shipped in: 2-3 Weeks

HiPurA Blood Genomic DNA Miniprep Purification Kit

HiPurA Blood Genomic DNA Miniprep Purification Kit

₹ 31,801.00

Brand: HIMEDIA

Catalog No.: MB504-250PR

Quantity/Unit: 250pr*/EA

Usually Shipped in: 2-3 Weeks

HiPurA Blood Genomic DNA Midiprep Purification Kit-MB516-10PR

HiPurA Blood Genomic DNA Midiprep Purification Kit-MB516-10PR

₹ 4,349.00

Brand: HIMEDIA

Catalog No.: MB516-10PR

Quantity/Unit: 10pr*/EA

Usually Shipped in: 2-3 Weeks

HiPurA Blood Genomic DNA Midiprep Purification Kit

HiPurA Blood Genomic DNA Midiprep Purification Kit

₹ 10,301.00

Brand: HIMEDIA

Catalog No.: MB516-25PR

Quantity/Unit: 25pr*/EA

Usually Shipped in: 2-3 Weeks

HiPurA Blood Genomic DNA Maxiprep Purification Kit-MB517-10PR

HiPurA Blood Genomic DNA Maxiprep Purification Kit-MB517-10PR

₹ 7,815.00

Brand: HIMEDIA

Catalog No.: MB517-10PR

Quantity/Unit: 10pr*/EA

Usually Shipped in: 2-3 Weeks

HiPurA Blood Genomic DNA Maxiprep Purification Kit

HiPurA Blood Genomic DNA Maxiprep Purification Kit

₹ 19,249.00

Brand: HIMEDIA

Catalog No.: MB517-25PR

Quantity/Unit: 25pr*/EA

Usually Shipped in: 2-3 Weeks

HiPurA 96 Blood Genomic DNA Purification Kit-MB521-1X96PR

HiPurA 96 Blood Genomic DNA Purification Kit-MB521-1X96PR

₹ 13,554.00

Brand: HIMEDIA

Catalog No.: MB521-1X96PR

Quantity/Unit: 1X96pr*/EA

Usually Shipped in: 2-3 Weeks

HiPurA Plant DNA Isolation Kit (CTAB Method)

HiPurA Plant DNA Isolation Kit (CTAB Method)

₹ 2,807.00

Brand: HIMEDIA

Catalog No.: MB502-50PR

Quantity/Unit: 50pr*/EA

Usually Shipped in: 2-3 Weeks

HiPurA Cotton DNA Isolation Kit-MB528-50PR

HiPurA Cotton DNA Isolation Kit-MB528-50PR

₹ 4,000.00

Brand: HIMEDIA

Catalog No.: MB528-50PR

Quantity/Unit: 50pr*/EA

Usually Shipped in: 2-3 Weeks

HiPurA Cotton DNA Isolation Kit

HiPurA Cotton DNA Isolation Kit

₹ 17,876.00

Brand: HIMEDIA

Catalog No.: MB528-250PR

Quantity/Unit: 250pr*/EA

Usually Shipped in: 2-3 Weeks