
DNA Purification and Extraction
Swiss Biologist Friedrich Miescher was the first person to isolate a DNA. The process refers to both extraction and purification of DNA from the given sample such as blood, swab, plant tissue, animal tissue, bacterial cells, etc. A general protocol involves homogenization of cells using a physical method like the use of mortar and pestle or automated homogenizer. This may be followed by a series of chemical treatment with detergents to remove fats, rupture cellular membranes and then by using Chloroform & further centrifugation, DNA can be separated from the cellular debris. A high temperature may be maintained to promote cell lysis. A buffer like Tris to maintain pH is used throughout. Ethanol or isopropanol is then used to precipitate out DNA. Purification may involve many steps like the use of proteinase or phenol-chloroform extraction to eliminate protein impurities or RNAse to remove RNA.
Kits for extraction and purification of DNA specific to the source of DNA are available for ready-to-use, or separate components can be used to develop a standardized protocol.
Geneasy Bacterial DNA lsolation Kit-BDIK 25
- Brand:
BHAT BIO-TECH INDIA PVT. LTD. - Catalog No.:
BDIK 25 - Quantity/Unit: 25 Reactions/Pack
- Usually Shipped in: 4-7 Days
Geneasy Bacterial DNA lsolation Kit-BDIK 50
- Brand:
BHAT BIO-TECH INDIA PVT. LTD. - Catalog No.:
BDIK 50 - Quantity/Unit: 50 Reactions/Pack
- Usually Shipped in: 4-7 Days
Geneasy Fungal DNA lsolation Kit-FDIK 25
- Brand:
BHAT BIO-TECH INDIA PVT. LTD. - Catalog No.:
FDIK 25 - Quantity/Unit: 25 Reactions/Pack
- Usually Shipped in: 4-7 Days
Geneasy Fungal DNA lsolation Kit-FDIK 50
- Brand:
BHAT BIO-TECH INDIA PVT. LTD. - Catalog No.:
FDIK 50 - Quantity/Unit: 50 Reactions/Pack
- Usually Shipped in: 4-7 Days
Geneasy Plant DNA lsolation Kit-PLDIK 25
- Brand:
BHAT BIO-TECH INDIA PVT. LTD. - Catalog No.:
PLDIK 25 - Quantity/Unit: 25 Reactions/Pack
- Usually Shipped in: 4-7 Days
Geneasy Plant DNA lsolation Kit-PLDIK 50
- Brand:
BHAT BIO-TECH INDIA PVT. LTD. - Catalog No.:
PLDIK 50 - Quantity/Unit: 50 Reactions/Pack
- Usually Shipped in: 4-7 Days
Geneasy Whole Blood/Mammalian Cells/Tissues DNA lsolation Kit-WBDIK 25
- Brand:
BHAT BIO-TECH INDIA PVT. LTD. - Catalog No.:
WBDIK 25 - Quantity/Unit: 25 Reactions/Pack
- Usually Shipped in: 4-7 Days
Geneasy Whole Blood/Mammalian Cells/Tissues DNA lsolation Kit-WBDIK 50
- Brand:
BHAT BIO-TECH INDIA PVT. LTD. - Catalog No.:
WBDIK 50 - Quantity/Unit: 50 Reactions/Pack
- Usually Shipped in: 4-7 Days
Geneasy Plasmid DNA lsolation Kit-PDIK 25
- Brand:
BHAT BIO-TECH INDIA PVT. LTD. - Catalog No.:
PDIK 25 - Quantity/Unit: 25 Reactions/Pack
- Usually Shipped in: 4-7 Days
Geneasy Plasmid DNA lsolation Kit-PDIK 50
- Brand:
BHAT BIO-TECH INDIA PVT. LTD. - Catalog No.:
PDIK 50 - Quantity/Unit: 50 Reactions/Pack
- Usually Shipped in: 4-7 Days
Geneasy DNA Gel Extraction Kit-DGEK 25
- Brand:
BHAT BIO-TECH INDIA PVT. LTD. - Catalog No.:
DGEK 25 - Quantity/Unit: 25 Reactions/Pack
- Usually Shipped in: 4-7 Days
Geneasy DNA Gel Extraction Kat-DGEK 50
- Brand:
BHAT BIO-TECH INDIA PVT. LTD. - Catalog No.:
DGEK 50 - Quantity/Unit: 50 Reactions/Pack
- Usually Shipped in: 4-7 Days
Geneasy PCR Product Purification Kit-PPPK 25
- Brand:
BHAT BIO-TECH INDIA PVT. LTD. - Catalog No.:
PPPK 25 - Quantity/Unit: 25 Reactions/Pack
- Usually Shipped in: 4-7 Days
Geneasy PCR Product Purification Kit-PPPK 50
- Brand:
BHAT BIO-TECH INDIA PVT. LTD. - Catalog No.:
PPPK 50 - Quantity/Unit: 50 Reactions/Pack
- Usually Shipped in: 4-7 Days
HiPer DNA Estimation Teaching Kit-HTBC006-20PR
₹ 9,290.00
- Brand:
HIMEDIA - Catalog No.:
HTBC006-20PR - Quantity/Unit: 20PR/Pack
- Usually Shipped in: 1-2 Weeks
HiPer Yeast Genomic DNA Extraction Teaching Kit-HTBM013-10PR
₹ 3,955.00
- Brand:
HIMEDIA - Catalog No.:
HTBM013-10PR - Quantity/Unit: 10PR/Pack
- Usually Shipped in: 1-2 Weeks
HiPer DNA Molecular Size Determination Teaching Kit-HTBM014-10PR
₹ 3,580.00
- Brand:
HIMEDIA - Catalog No.:
HTBM014-10PR - Quantity/Unit: 10PR/Pack
- Usually Shipped in: 1-2 Weeks
HiPer Plasmid DNA Cloning Teaching Kit-HTBM022-5PR
₹ 7,210.00
- Brand:
HIMEDIA - Catalog No.:
HTBM022-5PR - Quantity/Unit: 5PR/Pack
- Usually Shipped in: 1-2 Weeks
HiPer InstaDNA Teaching Kit-HTBM033-5PR
₹ 3,850.00
- Brand:
HIMEDIA - Catalog No.:
HTBM033-5PR - Quantity/Unit: 5PR/Pack
- Usually Shipped in: 1-2 Weeks
Insta NX Food Pathogen DNA Purification Kit-MBIN018-12PR
₹ 6,175.00
- Brand:
HIMEDIA - Catalog No.:
MBIN018-12PR - Quantity/Unit: 12PR/Pack
- Usually Shipped in: 1-2 Weeks
Insta NX Mycobacterium Tuberculosis DNA Purification Kit-MBIN019-12PR
₹ 6,175.00
- Brand:
HIMEDIA - Catalog No.:
MBIN019-12PR - Quantity/Unit: 12PR/Pack
- Usually Shipped in: 1-2 Weeks
Insta NX Forensic Multi-Sample DNA Purification Kit-MBIN020-12PR
₹ 9,265.00
- Brand:
HIMEDIA - Catalog No.:
MBIN020-12PR - Quantity/Unit: 12PR/Pack
- Usually Shipped in: 1-2 Weeks
HiPurA Spice DNA Purification Kit-MB567-50PR
₹ 7,735.00
- Brand:
HIMEDIA - Catalog No.:
MB567-50PR - Quantity/Unit: 50PR/Pack
- Usually Shipped in: 1-2 Weeks
HiPurA Urine DNA Purification Kit-MB573-20PR
₹ 2,740.00
- Brand:
HIMEDIA - Catalog No.:
MB573-20PR - Quantity/Unit: 20PR/Pack
- Usually Shipped in: 1-2 Weeks
HiPurA Urine DNA Purification Kit-MB573-50PR
₹ 6,040.00
- Brand:
HIMEDIA - Catalog No.:
MB573-50PR - Quantity/Unit: 50PR/Pack
- Usually Shipped in: 1-2 Weeks
HiPurA Urine DNA Purification Kit-MB573-250PR
₹ 26,840.00
- Brand:
HIMEDIA - Catalog No.:
MB573-250PR - Quantity/Unit: 250PR/Pack
- Usually Shipped in: 1-2 Weeks
HiPurA Viral DNA Purification Kit-MB575-20PR
₹ 5,230.00
- Brand:
HIMEDIA - Catalog No.:
MB575-20PR - Quantity/Unit: 20PR/Pack
- Usually Shipped in: 1-2 Weeks
HiPurA Viral DNA Purification Kit-MB575-50PR
₹ 11,310.00
- Brand:
HIMEDIA - Catalog No.:
MB575-50PR - Quantity/Unit: 50PR/Pack
- Usually Shipped in: 1-2 Weeks