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Protein Estimation

Protein estimation refers to measuring the total level of protein or the concentration of protein in a solution. It usually involves Biochemical assays in combination with Physical methods like spectrophotometry or colorimetry. One of the earliest method developed is that of Lowry protein assay. The Cu+ ions which are produced by the oxidation of peptide bonds (present in proteins) react with the FolinCiocalteu reagent in alkaline conditions. The reduction of the Folin reagent produces a color change in the solution. This change is proportional to the protein concentration and can easily be measured using colorimetry. The Bicinchoninic acid assay also known as BCA was a further development of the Lowry method. The stock BCA solution has all the required reagents and an alkaline pH of 11.25. Here, the bicinchoninic acid chelates with Cu+ ion forming a purple-colored complex. The protein of interest can be quantified by comparing the absorption spectra with the protein solutions of known concentration. The Bradford assay, on the other hand, is based on the absorbance shift of the dye Coomassie Brilliant Blue G-250 under an acidic condition where the red dye shifts into its bluer form on binding the protein being assayed.
 
Biomall offers a range of these reagents/solutions/ kits which are required for protein estimation and assays.

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1-Step ABTS Solution

1-Step ABTS Solution
₹ 16,116.00

Brand: Pierce Protein Research Products

Catalog No.: PI37615

Quantity/Unit: 250 ML/Bottle

Usually Shipped in: 3-4 Weeks

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1-Step Heavy Protein IVT Kit

1-Step Heavy Protein IVT Kit
₹ 1,36,665.00

Brand: Pierce Protein Research Products

Catalog No.: PI88331

Quantity/Unit: 40 reactions/Kit

Usually Shipped in: 3-4 Weeks

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1-Step Heavy Protein IVT Kit-PI88330

1-Step Heavy Protein IVT Kit-PI88330
₹ 41,063.00

Brand: Pierce Protein Research Products

Catalog No.: PI88330

Quantity/Unit: 8rxns/Kit

Usually Shipped in: 3-4 Weeks

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1-Step NBT/BCIP Solution

1-Step NBT/BCIP Solution
₹ 23,410.00

Brand: Pierce Protein Research Products

Catalog No.: PI34042

Quantity/Unit: 250 ML/Bottle

Usually Shipped in: 3-4 Weeks

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ActivX Azido-FP Serine Hydrolase Probe

ActivX Azido-FP Serine Hydrolase Probe
₹ 55,902.00

Brand: Pierce Protein Research Products

Catalog No.: PI88316

Quantity/Unit: 3.5UG/Pack

Usually Shipped in: 3-4 Weeks

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ActivX Desthiobiotin-ADP Probe

ActivX Desthiobiotin-ADP Probe
₹ 44,570.00

Brand: Pierce Protein Research Products

Catalog No.: PI88313

Quantity/Unit: 16 X 9.9 UG/Pack

Usually Shipped in: 3-4 Weeks

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ActivX Desthiobiotin-ATP Probe

ActivX Desthiobiotin-ATP Probe
₹ 44,570.00

Brand: Pierce Protein Research Products

Catalog No.: PI88311

Quantity/Unit: 16 X 12.6 UG/Pack

Usually Shipped in: 3-4 Weeks

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ActivX Desthiobiotin-FP Serine Hydrolase Probe

ActivX Desthiobiotin-FP Serine Hydrolase Probe
₹ 56,879.00

Brand: Pierce Protein Research Products

Catalog No.: PI88317

Quantity/Unit: 4.6 UG/EA

Usually Shipped in: 3-4 Weeks

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ActivX Desthiobiotin-GTP Probe

ActivX Desthiobiotin-GTP Probe
₹ 38,905.00

Brand: Pierce Protein Research Products

Catalog No.: PI88315

Quantity/Unit: 16 X 12.9 UG/Pack

Usually Shipped in: 3-4 Weeks

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Active Arf1 Pull-Down and Detection Kit

Active Arf1 Pull-Down and Detection Kit
₹ 1,19,630.00

Brand: Pierce Protein Research Products

Catalog No.: PI16121

Quantity/Unit: 30 tests/Kit

Usually Shipped in: 3-4 Weeks

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Active Cdc42 Pull-Down and Detection Kit

Active Cdc42 Pull-Down and Detection Kit
₹ 1,19,630.00

Brand: Pierce Protein Research Products

Catalog No.: PI16119

Quantity/Unit: 30 tests/Kit

Usually Shipped in: 3-4 Weeks

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Active Rac1 Pull-Down and Detection Kit

Active Rac1 Pull-Down and Detection Kit
₹ 1,19,630.00

Brand: Pierce Protein Research Products

Catalog No.: PI16118

Quantity/Unit: 30 tests/Kit

Usually Shipped in: 3-4 Weeks

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Active Rap1 Pull-Down and Detection Kit

Active Rap1 Pull-Down and Detection Kit
₹ 1,19,630.00

Brand: Pierce Protein Research Products

Catalog No.: PI16120

Quantity/Unit: 30 tests/Kit

Usually Shipped in: 3-4 Weeks

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Active Ras Pull-Down and Detection Kit

Active Ras Pull-Down and Detection Kit
₹ 68,486.00

Brand: Pierce Protein Research Products

Catalog No.: PI16117

Quantity/Unit: 30 tests/Kit

Usually Shipped in: 3-4 Weeks

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Avidin-PI21121

Avidin-PI21121
₹ 16,018.00

Brand: Pierce Protein Research Products

Catalog No.: PI21121

Quantity/Unit: 10 MG/Pack

Usually Shipped in: 3-4 Weeks

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