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Protein Estimation

Protein estimation refers to measuring the total level of protein or the concentration of protein in a solution. It usually involves Biochemical assays in combination with Physical methods like spectrophotometry or colorimetry. One of the earliest method developed is that of Lowry protein assay. The Cu+ ions which are produced by the oxidation of peptide bonds (present in proteins) react with the FolinCiocalteu reagent in alkaline conditions. The reduction of the Folin reagent produces a color change in the solution. This change is proportional to the protein concentration and can easily be measured using colorimetry. The Bicinchoninic acid assay also known as BCA was a further development of the Lowry method. The stock BCA solution has all the required reagents and an alkaline pH of 11.25. Here, the bicinchoninic acid chelates with Cu+ ion forming a purple-colored complex. The protein of interest can be quantified by comparing the absorption spectra with the protein solutions of known concentration. The Bradford assay, on the other hand, is based on the absorbance shift of the dye Coomassie Brilliant Blue G-250 under an acidic condition where the red dye shifts into its bluer form on binding the protein being assayed.

Biomall offers a range of these reagents/solutions/ kits which are required for protein estimation and assays.

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FACS Rinse Solution

Brand: BD Biosciences

Catalog No.: 340346

Quantity/Unit: 5 L/Bottle

Usually Shipped in: 3-4 Weeks

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Calibrite APC Beads

Brand: BD Biosciences

Catalog No.: 340487

Quantity/Unit: 25 Tests/Kit

Usually Shipped in: 3-4 Weeks

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CD117 APC (RUO)

Brand: BD Biosciences

Catalog No.: 341096

Quantity/Unit: 100 tests/Kit

Usually Shipped in: 3-4 Weeks

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CD45 PERCP

Brand: BD Biosciences

Catalog No.: 347464

Quantity/Unit: 100 Tests/Kit

Usually Shipped in: 3-4 Weeks

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CD19 (4G7) PERCP

Brand: BD Biosciences

Catalog No.: 347544

Quantity/Unit: 100 Tests/Kit

Usually Shipped in: 3-4 Weeks

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FACS LYSING SOLUTION (IVD/10X-500 TESTS)

Brand: BD Biosciences

Catalog No.: 349202

Quantity/Unit: 500 tests/Kit

Usually Shipped in: 3-4 Weeks

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DNA QC PARTICLES (25 TESTS)

Brand: BD Biosciences

Catalog No.: 349523

Quantity/Unit: 25 Tests/Kit

Usually Shipped in: 3-4 Weeks

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BCIP /NBT,50ml

₹ 3,338.00

Brand: Bangalore GeNei

Catalog No.: 621610280501730

Quantity/Unit: 50 ml/Bottle

Availability: In stock

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Ouchterlony Double Diffusion Teaching Kit for Antigen-Antibody patterns

₹ 5,192.00

Brand: Bangalore GeNei

Catalog No.: 6107000011730

Quantity/Unit: 15 experiments/Kit

Usually Shipped in: 2-3 Weeks

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Ouchterlony Double Diffusion Teaching Kit for Antibody Titration

₹ 3,344.00

Brand: Bangalore GeNei

Catalog No.: 6100900011730

Quantity/Unit: 15 experiments/Kit

Usually Shipped in: 2-3 Weeks

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