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Protein Estimation

Protein Estimation

Protein estimation refers to measuring the total level of protein or the concentration of protein in a solution. It usually involves Biochemical assays in combination with Physical methods like spectrophotometry or colorimetry. One of the earliest method developed is that of Lowry protein assay. The Cu+ ions which are produced by the oxidation of peptide bonds (present in proteins) react with the FolinCiocalteu reagent in alkaline conditions. The reduction of the Folin reagent produces a color change in the solution. This change is proportional to the protein concentration and can easily be measured using colorimetry. The Bicinchoninic acid assay also known as BCA was a further development of the Lowry method. The stock BCA solution has all the required reagents and an alkaline pH of 11.25. Here, the bicinchoninic acid chelates with Cu+ ion forming a purple-colored complex. The protein of interest can be quantified by comparing the absorption spectra with the protein solutions of known concentration. The Bradford assay, on the other hand, is based on the absorbance shift of the dye Coomassie Brilliant Blue G-250 under an acidic condition where the red dye shifts into its bluer form on binding the protein being assayed.
 
Biomall offers a range of these reagents/solutions/ kits which are required for protein estimation and assays.
BCIP /NBT,50ml
₹ 3,338.00
  • Brand: Bangalore GeNei
  • Catalog No.: 621610280501730
  • Quantity/Unit: 50 ml/Bottle
Ouchterlony Double Diffusion Teaching Kit for Antigen-Antibody patterns
₹ 5,192.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6107000011730
  • Quantity/Unit: 15 experiments/Kit
Ouchterlony Double Diffusion Teaching Kit for Antibody Titration
₹ 3,344.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6100900011730
  • Quantity/Unit: 15 experiments/Kit
Protein Estimation Kit by Bradford Macro Method-2603300011730
₹ 4,840.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2603300011730
  • Quantity/Unit: 250 reactions/Kit
Radial Immuno Diffusion Teaching Kit
₹ 3,960.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6101000011730
  • Quantity/Unit: 15 experiments/Kit
Bradford Protein Assay Kit-BCSK3031
₹ 5,200.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BCSK3031
  • Quantity/Unit: 1000 Assays/Kit
  • Usually Shipped in: 3-4 Weeks
Better Bradford Protein Assay Kit-BCSK3041
₹ 7,058.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BCSK3041
  • Quantity/Unit: 1000 Assays/Kit
  • Usually Shipped in: 3-4 Weeks
BCA Protein Assay Kit-BCSK3021
₹ 12,072.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BCSK3021
  • Quantity/Unit: 500 Assays/Kit
  • Usually Shipped in: 3-4 Weeks
Better BCA Protein Assay Kit-BCSK3051
₹ 13,744.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BCSK3051
  • Quantity/Unit: 500 Assays/Kit
  • Usually Shipped in: 3-4 Weeks
Micro BCA Protein Assay Kit-BCSK3061
₹ 9,100.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BCSK3061
  • Quantity/Unit: 1250 Assays/Kit
  • Usually Shipped in: 3-4 Weeks
Lowry Protein Assay Kit-BCSK4031
₹ 4,829.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BCSK4031
  • Quantity/Unit: 1000 Assays/Kit
  • Usually Shipped in: 3-4 Weeks
Stable Lowry Protein Assay Kit-BCSK4051
₹ 7,058.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BCSK4051
  • Quantity/Unit: 1000 Assays/Kit
  • Usually Shipped in: 3-4 Weeks
Modified Lowry Protein Assay Kit-BCSK4041
₹ 5,200.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BCSK4041
  • Quantity/Unit: 1000 Assays/Kit
  • Usually Shipped in: 3-4 Weeks
Phosphoprotein Phosphate Estimation Kit-BCBSP061
₹ 8,915.00
  • Brand: BR Biochem Life Sciences
  • Catalog No.: BCBSP061
  • Quantity/Unit: 2000 Assays/Kit
  • Usually Shipped in: 3-4 Weeks
Protein Estimation Kit by Lowrys Method-2601800011730
₹ 3,861.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2601800011730
  • Quantity/Unit: 250 reactions/Kit
Protein Estimation Kit by BCA Method-2603100011730
₹ 12,903.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2603100011730
  • Quantity/Unit: 250 reactions/Kit
GeNei Protein Estimation Kit-2624800021730
₹ 9,504.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2624800021730
  • Quantity/Unit: 250 reactions/Kit
Protein Estimation Kit by Biuret method
₹ 7,084.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2601900011730
  • Quantity/Unit: 250 rxns/Pack