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Protein Estimation

Protein estimation refers to measuring the total level of protein or the concentration of protein in a solution. It usually involves Biochemical assays in combination with Physical methods like spectrophotometry or colorimetry. One of the earliest method developed is that of Lowry protein assay. The Cu+ ions which are produced by the oxidation of peptide bonds (present in proteins) react with the FolinCiocalteu reagent in alkaline conditions. The reduction of the Folin reagent produces a color change in the solution. This change is proportional to the protein concentration and can easily be measured using colorimetry. The Bicinchoninic acid assay also known as BCA was a further development of the Lowry method. The stock BCA solution has all the required reagents and an alkaline pH of 11.25. Here, the bicinchoninic acid chelates with Cu+ ion forming a purple-colored complex. The protein of interest can be quantified by comparing the absorption spectra with the protein solutions of known concentration. The Bradford assay, on the other hand, is based on the absorbance shift of the dye Coomassie Brilliant Blue G-250 under an acidic condition where the red dye shifts into its bluer form on binding the protein being assayed.
 
Biomall offers a range of these reagents/solutions/ kits which are required for protein estimation and assays.

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BCA Protein Assay Kit-BCSK3021

BCA Protein Assay Kit-BCSK3021

₹ 12,072.00

Brand: BR Biochem Life Sciences

Catalog No.: BCSK3021

Quantity/Unit: 500 Assays/Kit

Usually Shipped in: 3-4 Weeks

BCA, disodium salt-BC0260-25g

BCA, disodium salt-BC0260-25g

₹ 1,05,863.00

Brand: BR Biochem Life Sciences

Catalog No.: BC0260-25g

Quantity/Unit: 25g/Pack

Usually Shipped in: 3-4 Weeks

BCA, disodium salt-BC0260-5g

BCA, disodium salt-BC0260-5g

₹ 22,287.00

Brand: BR Biochem Life Sciences

Catalog No.: BC0260-5g

Quantity/Unit: 5g/Pack

Usually Shipped in: 3-4 Weeks

BCIP /NBT,50ml

BCIP /NBT,50ml

₹ 3,338.00

Brand: Bangalore GeNei

Catalog No.: 621610280501730

Quantity/Unit: 50 ml/Bottle

Availability: In stock

BCIP toluidine salt-BC0072-100mg

BCIP toluidine salt-BC0072-100mg

₹ 4,457.00

Brand: BR Biochem Life Sciences

Catalog No.: BC0072-100mg

Quantity/Unit: 100mg/Pack

Usually Shipped in: 3-4 Weeks

BCIP, disodium salt-BC1171-100mg

BCIP, disodium salt-BC1171-100mg

₹ 9,286.00

Brand: BR Biochem Life Sciences

Catalog No.: BC1171-100mg

Quantity/Unit: 100mg/Pack

Usually Shipped in: 3-4 Weeks

BCIP, disodium salt-BC1171-500mg

BCIP, disodium salt-BC1171-500mg

₹ 39,002.00

Brand: BR Biochem Life Sciences

Catalog No.: BC1171-500mg

Quantity/Unit: 500mg/Pack

Usually Shipped in: 3-4 Weeks

Better BCA Protein Assay Kit-BCSK3051

Better BCA Protein Assay Kit-BCSK3051

₹ 13,744.00

Brand: BR Biochem Life Sciences

Catalog No.: BCSK3051

Quantity/Unit: 500 Assays/Kit

Usually Shipped in: 3-4 Weeks

Better Bradford Protein Assay Kit-BCSK3041

Better Bradford Protein Assay Kit-BCSK3041

₹ 7,058.00

Brand: BR Biochem Life Sciences

Catalog No.: BCSK3041

Quantity/Unit: 1000 Assays/Kit

Usually Shipped in: 3-4 Weeks

Bradford Protein Assay Kit-BCSK3031

Bradford Protein Assay Kit-BCSK3031

₹ 5,200.00

Brand: BR Biochem Life Sciences

Catalog No.: BCSK3031

Quantity/Unit: 1000 Assays/Kit

Usually Shipped in: 3-4 Weeks

Bradford Reagent-BC0641-100ml

Bradford Reagent-BC0641-100ml

₹ 2,550.00

Brand: BR Biochem Life Sciences

Catalog No.: BC0641-100ml

Quantity/Unit: 100ml/Pack

Usually Shipped in: 3-4 Weeks

CD117 APC (RUO)

CD117 APC (RUO)

Brand: BD Biosciences

Catalog No.: 341096

Quantity/Unit: 100 tests/Kit

Usually Shipped in: 3-4 Weeks

CD19 (4G7) PERCP

CD19 (4G7) PERCP

Brand: BD Biosciences

Catalog No.: 347544

Quantity/Unit: 100 Tests/Kit

Usually Shipped in: 3-4 Weeks

CD45 PERCP

CD45 PERCP

Brand: BD Biosciences

Catalog No.: 347464

Quantity/Unit: 100 Tests/Kit

Usually Shipped in: 3-4 Weeks

Calibrite APC Beads

Calibrite APC Beads

Brand: BD Biosciences

Catalog No.: 340487

Quantity/Unit: 25 Tests/Kit

Usually Shipped in: 3-4 Weeks