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PCR

PCR

Polymerase chain reaction or PCR is a technique developed by American biochemist Kary Mullis to amplify a DNA of interest. In a conventional PCR reaction, the first step is the heat-denaturation of the double-stranded DNA at 94 to 96C. This is followed by annealing of the specifically designed primer at the target site and then polymerase catalyzed elongation by the addition of the nucleotides. The denaturation takes about 30 secs, the annealing about 20 to 40 secs and the elongation takes several minutes depending upon the number of cycles set in the PCR machine. PCR machine, DNA template, a polymerase enzyme (usually the heat-resistant Taq polymerase), specifically designed forward and reverse primers, the deoxynucleoside triphosphates or dNTPs mixture which will provide the nucleotides for elongation - they act as the building blocks of the new strand, and the buffer solution to maintain the pH, are the key requirements. The reaction is carried out in 0.1 ml / 0.2 ml PCR tubes or specially designed PCR plates. PCR Kits are also available with everything readymade and quick to use.


In addition to the conventional PCR, a gradient PCR with a range of variation in annealing temperature can also be used. It is Ideal when while developing the protocol for a new DNA sample. Quantitative PCR (qPCR) which uses fluorescent dyes like Sybr Green can be performed to measure the quantity of the amplified products in real-time.

Sealing Films, Polyester, Type-Clear Seal Film
₹ 15,729.00
  • Brand: ABDOS
  • Catalog No.: P10718
  • Quantity/Unit: 100/Case
  • Usually Shipped in: 1-2 Weeks
Deep Well Plates Family, PP, Capacity (ml)-2.2, Type-Deep Well Plates
₹ 9,830.62
  • Brand: ABDOS
  • Catalog No.: P10802
  • Quantity/Unit: 25/Case
  • Usually Shipped in: 1-2 Weeks
Strip Caps, PP, Capacity-0.2 ml, Type-8 Strip Cap Flat Optically Clear
₹ 1,822.59
  • Brand: ABDOS
  • Catalog No.: P10709
  • Quantity/Unit: 125/Case
  • Usually Shipped in: 1-2 Weeks
PCR Tubes, PP, Capacity-0.2 ml, Type-Clear Flat Cap
₹ 2,961.00
  • Brand: ABDOS
  • Catalog No.: P10701
  • Quantity/Unit: 1000/Case
  • Usually Shipped in: 1-2 Weeks
Maxiamp 0.1ml Low Profile Tube Strips with Cap-611010
₹ 4,340.00
  • Brand: TARSONS
  • Catalog No.: 611010
  • Quantity/Unit: 125/Pack
  • Usually Shipped in: 1-2 Weeks
Deep Well Storage Plates - 2.0 ml -510068
₹ 25,189.00
  • Brand: TARSONS
  • Catalog No.: 510068
  • Quantity/Unit: 50/Pack
  • Usually Shipped in: 1-2 Weeks
InoMag PCR plates non Skited 0.2ml
₹ 1,300.00
  • Brand: ILS
  • Catalog No.: 8007
  • Quantity/Unit: 10 piece/Pack
  • Usually Shipped in: 4-7 Days
96 Well Fully Skirted Low Profile PCR Plate
₹ 3,182.31
  • Brand: ABDOS
  • Catalog No.: P10723
  • Quantity/Unit: 10/Case
  • Usually Shipped in: 1-2 Weeks
Maxiamp 0.1ml Low Profile Tube Strips with Cap-611020
₹ 4,745.00
  • Brand: TARSONS
  • Catalog No.: 611020
  • Quantity/Unit: 125/Pack
  • Usually Shipped in: 1-2 Weeks
Maxiamp 0.2ml Tubes Strips with Cap -610060
₹ 4,745.00
  • Brand: TARSONS
  • Catalog No.: 610060
  • Quantity/Unit: 125/Pack
  • Usually Shipped in: 1-2 Weeks
Maxiamp 0.2ml Tubes Strips with attached Cap -610090
₹ 5,692.00
  • Brand: TARSONS
  • Catalog No.: 610090
  • Quantity/Unit: 120/Pack
  • Usually Shipped in: 1-2 Weeks
Maxiamp 0.1ml Low Profile Tube Strips with Cap-611040
₹ 5,695.00
  • Brand: TARSONS
  • Catalog No.: 611040
  • Quantity/Unit: 125/Pack
  • Usually Shipped in: 1-2 Weeks
Maxiamp 0.2ml Tubes Strips with attached Cap -610080
₹ 5,285.00
  • Brand: TARSONS
  • Catalog No.: 610080
  • Quantity/Unit: 120/Pack
  • Usually Shipped in: 1-2 Weeks
Teleshake 95 of INHECO with centering plate mounted stationary temper shakingunit
₹ 0.00
  • Brand: BRAND
  • Catalog No.: 709470
  • Quantity/Unit: 1 EA/Pack
  • Usually Shipped in: 5-6 Weeks
PCR Mini Cooler, PP, with transparent lid
₹ 7,803.00
  • Brand: BRAND
  • Catalog No.: 781260
  • Quantity/Unit: 2/Pack
  • Usually Shipped in: 5-6 Weeks
RESERVOIR 96WELL 220 ML
₹ 0.00
  • Brand: BRAND
  • Catalog No.: 701450
  • Quantity/Unit: 10 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
RESERVOIR 12 X 6 ML
₹ 0.00
  • Brand: BRAND
  • Catalog No.: 701452
  • Quantity/Unit: 10 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
RESERVOIR 4 X 60 ML
₹ 0.00
  • Brand: BRAND
  • Catalog No.: 701454
  • Quantity/Unit: 10 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
RESERVOIR 6 X 40 ML
₹ 0.00
  • Brand: BRAND
  • Catalog No.: 701456
  • Quantity/Unit: 10 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
RESERVOIR 50 ML
₹ 0.00
  • Brand: BRAND
  • Catalog No.: 701458
  • Quantity/Unit: 10 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
BRAND Seal-R-Film sealing film, length 15 mtrs, with 500 mm
₹ 0.00
  • Brand: BRAND
  • Catalog No.: 701628
  • Quantity/Unit: 6/Pack
  • Usually Shipped in: 5-6 Weeks
Sealing paddle
₹ 9,968.00
  • Brand: BRAND
  • Catalog No.: 781381
  • Quantity/Unit: 100 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
Foilstrips Aluminum, self adhesive, for PCR and storage
₹ 5,722.00
  • Brand: BRAND
  • Catalog No.: 781382
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
Filmstrips polyester, self adhesive, for PCR, qPCR and storage
₹ 7,673.00
  • Brand: BRAND
  • Catalog No.: 781383
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
Sealing film, Polyester, self-adhesive, for ELISA and PCR
₹ 9,763.00
  • Brand: BRAND
  • Catalog No.: 781390
  • Quantity/Unit: 100 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
Ground joint sleeves, PTFE, for NS 29/32, thin-walled
₹ 2,924.00
  • Brand: BRAND
  • Catalog No.: 51422
  • Quantity/Unit: 10/Pack
  • Usually Shipped in: 5-6 Weeks
Ground socket, PTFE NS 29/32, grip collar and sealing rings
₹ 2,589.00
  • Brand: BRAND
  • Catalog No.: 51467
  • Quantity/Unit: 1/Pack
  • Usually Shipped in: 5-6 Weeks
Mat cover, Silicone, for deep well plate 384-well, 0.3 ml
₹ 34,309.00
  • Brand: BRAND
  • Catalog No.: 701357
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks