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PCR

Polymerase chain reaction or PCR is a technique developed by American biochemist Kary Mullis to amplify a DNA of interest. In a conventional PCR reaction, the first step is heat-denaturation of the double-stranded DNA at 94 to 96C. This is followed by annealing of the specifically designed primer at the target site and then polymerase catalyzed elongation by the addition of the nucleotides. The denaturation takes about 30 secs, the annealing about 20 to 40 secs and the elongation takes several minutes depending upon the number of cycles set in the PCR machine. PCR machine, DNA template, a polymerase enzyme (usually the heat-resistant Taq polymerase), specifically designed forward and reverse primers, the deoxynucleoside triphosphates or dNTPs mixture which will provide the nucleotides for elongation - they act as the building blocks of the new strand, and the buffer solution to maintain the pH, are the key requirements. The reaction is carried out in 0.1 ml / 0.2 ml PCR tubes or specially designed PCR plates. PCR Kits are also available with everything readymade and quick to use.


In addition to the conventional PCR, a gradient PCR with a range of variation in annealing temperature can also be used. It is Ideal when while developing the protocol for a new DNA sample. Quantitative PCR (qPCR) which uses fluorescent dyes like Sybr Green can be performed to measure the quantity of amplified product in real time.

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PRODUCTS

 SYBR Green qPCR Master Mix-695100011730

SYBR Green qPCR Master Mix-695100011730

₹ 12,035.00

Brand: Bangalore GeNei

Catalog No.: 695100011730

Quantity/Unit: 200 x 25 µL rxns/Pack

Usually Shipped in: 4-7 Days

 Strips of 8 Flat Caps

Strips of 8 Flat Caps

₹ 14,032.00

Brand: ABgene

Catalog No.: AB0786

Quantity/Unit: 25 packs of 12/Case

Usually Shipped in: 3-4 Weeks

(STWSSV50) Single Tube WSSV Detection Kit-680300011730

(STWSSV50) Single Tube WSSV Detection Kit-680300011730

₹ 19,800.00

Brand: Bangalore GeNei

Catalog No.: 680300011730

Quantity/Unit: 50 tests/Kit

Usually Shipped in: 4-7 Days

(WSS V100A) WSSV Detection Kit-680500051730

(WSS V100A) WSSV Detection Kit-680500051730

₹ 37,070.00

Brand: Bangalore GeNei

Catalog No.: 680500051730

Quantity/Unit: 100 tests/Kit

Usually Shipped in: 4-7 Days

0.2mL Domed Cap Tubes

0.2mL Domed Cap Tubes

₹ 37,070.00

Brand: ABgene

Catalog No.: AB0370

Quantity/Unit: 10 packs of 100/Case

Usually Shipped in: 3-4 Weeks

0.2ml Flat Cap Clear Tubes-130063

0.2ml Flat Cap Clear Tubes-130063

₹ 37,070.00

Brand: Bangalore GeNei

Catalog No.: 130063

Quantity/Unit: 1000 Tubes/Pack