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PCR

Polymerase chain reaction or PCR is a technique developed by American biochemist Kary Mullis to amplify a DNA of interest. In a conventional PCR reaction, the first step is the heat-denaturation of the double-stranded DNA at 94 to 96C. This is followed by annealing of the specifically designed primer at the target site and then polymerase catalyzed elongation by the addition of the nucleotides. The denaturation takes about 30 secs, the annealing about 20 to 40 secs and the elongation takes several minutes depending upon the number of cycles set in the PCR machine. PCR machine, DNA template, a polymerase enzyme (usually the heat-resistant Taq polymerase), specifically designed forward and reverse primers, the deoxynucleoside triphosphates or dNTPs mixture which will provide the nucleotides for elongation - they act as the building blocks of the new strand, and the buffer solution to maintain the pH, are the key requirements. The reaction is carried out in 0.1 ml / 0.2 ml PCR tubes or specially designed PCR plates. PCR Kits are also available with everything readymade and quick to use.


In addition to the conventional PCR, a gradient PCR with a range of variation in annealing temperature can also be used. It is Ideal when while developing the protocol for a new DNA sample. Quantitative PCR (qPCR) which uses fluorescent dyes like Sybr Green can be performed to measure the quantity of the amplified products in real-time.

PRODUCTS

DNAmp THERMAL CYCLER-DNAmp-20

DNAmp THERMAL CYCLER-DNAmp-20

₹ 1,39,000.00

Brand: BHAT BIO-TECH INDIA PVT. LTD.

Catalog No.: DNAmp-20

Quantity/Unit: 1 EA/Box

Usually Shipped in: 4-7 Days

Mini PCR Workstation(29 x 13 cm) -107211GB

Mini PCR Workstation(29 x 13 cm) -107211GB

₹ 17,468.00

Brand: Bangalore GeNei

Catalog No.: 107211GB

Quantity/Unit: 1EA/Box

Usually Shipped in: 2-3 Weeks

Midi PCR Workstation (45x60x30) cm-107214GB

Midi PCR Workstation (45x60x30) cm-107214GB

₹ 61,732.00

Brand: Bangalore GeNei

Catalog No.: 107214GB

Quantity/Unit: 1EA/Box

Usually Shipped in: 2-3 Weeks

GeNeiTM Nested PCR Teaching Kit, 5 Expets

GeNeiTM Nested PCR Teaching Kit, 5 Expets

₹ 9,493.00

Brand: Bangalore GeNei

Catalog No.: 6111100011730

Quantity/Unit: 1 EA/PACK

PCR Purification Kit- MPK4

PCR Purification Kit- MPK4

₹ 2,100.00

Brand: Aura Biotech

Catalog No.: MPK4

Quantity/Unit: 20 Rxns/Pack

Usually Shipped in: 1-3 Days

PCR Purification Kit- MPK5

PCR Purification Kit- MPK5

₹ 4,900.00

Brand: Aura Biotech

Catalog No.: MPK5

Quantity/Unit: 50 Rxns/Pack

Usually Shipped in: 1-3 Days

PCR Purification Kit- MPK6

PCR Purification Kit- MPK6

₹ 16,500.00

Brand: Aura Biotech

Catalog No.: MPK6

Quantity/Unit: 200 Rxns/Pack

Usually Shipped in: 1-3 Days

RT-PCR Teaching Kit- 5 reactions

RT-PCR Teaching Kit- 5 reactions

₹ 5,500.00

Brand: Aura Biotech

Catalog No.: MPL-02

Quantity/Unit: 5 Rxns/Pack

Usually Shipped in: 1-3 Days

RT-PCR Teaching Kit- 10 Reactions

RT-PCR Teaching Kit- 10 Reactions

₹ 10,000.00

Brand: Aura Biotech

Catalog No.: MPL-02M

Quantity/Unit: 10 Rxns/Pack

Usually Shipped in: 1-3 Days

MultiPlex PCR Teaching Kit

MultiPlex PCR Teaching Kit

₹ 4,200.00

Brand: Aura Biotech

Catalog No.: MPL-14

Quantity/Unit: 10 Rxns/Pack

Usually Shipped in: 1-3 Days

Real-Time PCR Teaching Kit

Real-Time PCR Teaching Kit

₹ 6,200.00

Brand: Aura Biotech

Catalog No.: MPL-15

Quantity/Unit: 10 Rxns/Pack

Usually Shipped in: 1-3 Days

Reverse Transcriptase- MPR-1S

Reverse Transcriptase- MPR-1S

₹ 3,000.00

Brand: Aura Biotech

Catalog No.: MPR-1S

Quantity/Unit: 5000 U/Pack

Usually Shipped in: 1-3 Days

Reverse Transcriptase- MPR-1

Reverse Transcriptase- MPR-1

₹ 5,450.00

Brand: Aura Biotech

Catalog No.: MPR-1

Quantity/Unit: 10000 U/Pack

Usually Shipped in: 1-3 Days

cDNA Synthesis Kit- MPC-1

cDNA Synthesis Kit- MPC-1

₹ 4,500.00

Brand: Aura Biotech

Catalog No.: MPC-1

Quantity/Unit: 25 Rxns/Pack

Usually Shipped in: 1-3 Days

cDNA Synthesis Kit- MPC-2

cDNA Synthesis Kit- MPC-2

₹ 7,500.00

Brand: Aura Biotech

Catalog No.: MPC-2

Quantity/Unit: 50 Rxns/Pack

Usually Shipped in: 1-3 Days