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PCR

PCR

Polymerase chain reaction or PCR is a technique developed by American biochemist Kary Mullis to amplify a DNA of interest. In a conventional PCR reaction, the first step is the heat-denaturation of the double-stranded DNA at 94 to 96C. This is followed by annealing of the specifically designed primer at the target site and then polymerase catalyzed elongation by the addition of the nucleotides. The denaturation takes about 30 secs, the annealing about 20 to 40 secs and the elongation takes several minutes depending upon the number of cycles set in the PCR machine. PCR machine, DNA template, a polymerase enzyme (usually the heat-resistant Taq polymerase), specifically designed forward and reverse primers, the deoxynucleoside triphosphates or dNTPs mixture which will provide the nucleotides for elongation - they act as the building blocks of the new strand, and the buffer solution to maintain the pH, are the key requirements. The reaction is carried out in 0.1 ml / 0.2 ml PCR tubes or specially designed PCR plates. PCR Kits are also available with everything readymade and quick to use.


In addition to the conventional PCR, a gradient PCR with a range of variation in annealing temperature can also be used. It is Ideal when while developing the protocol for a new DNA sample. Quantitative PCR (qPCR) which uses fluorescent dyes like Sybr Green can be performed to measure the quantity of the amplified products in real-time.

2X RED PCR Master Mix- 2x1.25ml

Brand: Aura Biotech

₹ 1,487.50 ₹ 1,750.00
15% Off
  • Brand: Aura Biotech
  • Catalog No.: MPE-6RM
  • Quantity/Unit: 2x1.25 ml/Pack
  • Usually Shipped in: 1-3 Days
PCR tubes, PP, 0.2 ml, BIO-CERT PCR QUALITY, attached domed cap, transparent

Brand: BRAND

₹ 4,518.00
  • Brand: BRAND
  • Catalog No.: 781300
  • Quantity/Unit: 1000/Pack
  • Usually Shipped in: 5-6 Weeks
PCR tubes, PP, 0.2 ml, BIO-CERT PCR QUALITY, attached flat cap, rose

Brand: BRAND

₹ 3,806.00
  • Brand: BRAND
  • Catalog No.: 781301
  • Quantity/Unit: 1000/Pack
  • Usually Shipped in: 5-6 Weeks
PCR tubes, PP, 0.2 ml, BIO-CERT PCR QUALITY, attached flat cap, yellow

Brand: BRAND

₹ 3,806.00
  • Brand: BRAND
  • Catalog No.: 781302
  • Quantity/Unit: 1000/Pack
  • Usually Shipped in: 5-6 Weeks
PCR tubes, PP, 0.2 ml, BIO-CERT PCR QUALITY, attached flat cap, green

Brand: BRAND

₹ 3,806.00
  • Brand: BRAND
  • Catalog No.: 781303
  • Quantity/Unit: 1000/Pack
  • Usually Shipped in: 5-6 Weeks
PCR tubes, PP, 0.2 ml, BIO-CERT PCR QUALITY, attached flat cap, blue

Brand: BRAND

₹ 3,806.00
  • Brand: BRAND
  • Catalog No.: 781304
  • Quantity/Unit: 1000/Pack
  • Usually Shipped in: 5-6 Weeks
PCR tubes, PP, 0.2 ml, BIO-CERT PCR QUALITY, attached flat cap, transparent

Brand: BRAND

₹ 3,625.00
  • Brand: BRAND
  • Catalog No.: 781305
  • Quantity/Unit: 1000/Pack
  • Usually Shipped in: 5-6 Weeks
PCR tubes, PP, 0.5 ml, BIO-CERT PCR QUALITY, attached flat cap, transparent

Brand: BRAND

₹ 4,136.00
  • Brand: BRAND
  • Catalog No.: 781310
  • Quantity/Unit: 1000/Pack
  • Usually Shipped in: 5-6 Weeks
PCR tubes, PP, 0.5 ml, BIO-CERT PCR QUALITY, attached flat cap, rose

Brand: BRAND

₹ 4,257.00
  • Brand: BRAND
  • Catalog No.: 781311
  • Quantity/Unit: 1000/Pack
  • Usually Shipped in: 5-6 Weeks
PCR tubes, PP, 0.5 ml, BIO-CERT PCR QUALITY, attached flat cap, yellow

Brand: BRAND

₹ 4,257.00
  • Brand: BRAND
  • Catalog No.: 781312
  • Quantity/Unit: 1000/Pack
  • Usually Shipped in: 5-6 Weeks
PCR tubes, PP, 0.5 ml, BIO-CERT PCR QUALITY, attached flat cap, green

Brand: BRAND

₹ 4,257.00
  • Brand: BRAND
  • Catalog No.: 781313
  • Quantity/Unit: 1000/Pack
  • Usually Shipped in: 5-6 Weeks
PCR tubes, PP, 0.5 ml, BIO-CERT PCR QUALITY, attached flat cap, blue

Brand: BRAND

₹ 4,257.00
  • Brand: BRAND
  • Catalog No.: 781314
  • Quantity/Unit: 1000/Pack
  • Usually Shipped in: 5-6 Weeks
PCR Mini Cooler, PP, with transparent lid

Brand: BRAND

₹ 8,440.00
  • Brand: BRAND
  • Catalog No.: 781260
  • Quantity/Unit: 2/Pack
  • Usually Shipped in: 5-6 Weeks
PCR-Box/-Rack, PP, for 0.2 ml single tubes, sorted by colors

Brand: BRAND

₹ 3,775.00
  • Brand: BRAND
  • Catalog No.: 781362
  • Quantity/Unit: 5 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
PCR Set 1: 50x PCR plates, 96-well, non-skirted, low profile, transparent+50x sealing films

Brand: BRAND

₹ 24,724.00
  • Brand: BRAND
  • Catalog No.: 781230
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
PCR Set 2: 50x PCR plates, 96-well, non-skirted, standard profile, transparent +50x sealing films

Brand: BRAND

₹ 24,724.00
  • Brand: BRAND
  • Catalog No.: 781231
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
PCR Set 3: 50x PCR plates, 96-well, semi-skirted, low profile, transparent +50x sealing films

Brand: BRAND

₹ 24,724.00
  • Brand: BRAND
  • Catalog No.: 781232
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
PCR Set 4: 50x PCR plates, 96-well, semi-skirted, raised skirt, low profile, transparent+50x sealing films

Brand: BRAND

₹ 24,724.00
  • Brand: BRAND
  • Catalog No.: 781233
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
PCR Set 5: 50x PCR plates, 96-well, semi-skirted, standard profile, transparent+50x sealing films

Brand: BRAND

₹ 24,724.00
  • Brand: BRAND
  • Catalog No.: 781234
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
384-well PCR plate, 0.03 ml, BIO-CERT PCR QUALITY, flexible, full skirt

Brand: BRAND

₹ 28,505.00
  • Brand: BRAND
  • Catalog No.: 781345
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
384-well PCR plate, 0.03 ml, BIO-CERT PCR QUALITY, flexible, full skirt, for qPCR

Brand: BRAND

₹ 21,354.00
  • Brand: BRAND
  • Catalog No.: 781347
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
96-well PCR plate, non-skirted, standard profile, elevated rim, white, blue coding

Brand: BRAND

₹ 14,616.00
  • Brand: BRAND
  • Catalog No.: 781350
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
96-well PCR plate, non-skirted, standard profile, elevated rim, white, blue coding

Brand: BRAND

₹ 16,647.00
  • Brand: BRAND
  • Catalog No.: 781354
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
96-well PCR plate, semi-skirted, standard profile, elevated rim, white, blue coding

Brand: BRAND

₹ 16,647.00
  • Brand: BRAND
  • Catalog No.: 781357
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
384-well PCR-plates, PP, white, 0.03 ml, BIO-CERT PCR QUALITY

Brand: BRAND

₹ 30,555.00
  • Brand: BRAND
  • Catalog No.: 781358
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
96-well PCR plate, semi-skirted, white, 0.15 ml+50x sealing films

Brand: BRAND

₹ 27,977.00
  • Brand: BRAND
  • Catalog No.: 781365
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
96-well PCR plate, non-skirted, low profile, transparent, blue coding

Brand: BRAND

₹ 13,985.00
  • Brand: BRAND
  • Catalog No.: 781366
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks
96-well PCR plate, non-skirted, low profile, white, blue coding

Brand: BRAND

₹ 16,287.00
  • Brand: BRAND
  • Catalog No.: 781367
  • Quantity/Unit: 50 pieces/Pack
  • Usually Shipped in: 5-6 Weeks