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PCR

Polymerase chain reaction or PCR is a technique developed by American biochemist Kary Mullis to amplify a DNA of interest. In a conventional PCR reaction, the first step is the heat-denaturation of the double-stranded DNA at 94 to 96C. This is followed by annealing of the specifically designed primer at the target site and then polymerase catalyzed elongation by the addition of the nucleotides. The denaturation takes about 30 secs, the annealing about 20 to 40 secs and the elongation takes several minutes depending upon the number of cycles set in the PCR machine. PCR machine, DNA template, a polymerase enzyme (usually the heat-resistant Taq polymerase), specifically designed forward and reverse primers, the deoxynucleoside triphosphates or dNTPs mixture which will provide the nucleotides for elongation - they act as the building blocks of the new strand, and the buffer solution to maintain the pH, are the key requirements. The reaction is carried out in 0.1 ml / 0.2 ml PCR tubes or specially designed PCR plates. PCR Kits are also available with everything readymade and quick to use.


In addition to the conventional PCR, a gradient PCR with a range of variation in annealing temperature can also be used. It is Ideal when while developing the protocol for a new DNA sample. Quantitative PCR (qPCR) which uses fluorescent dyes like Sybr Green can be performed to measure the quantity of the amplified products in real-time.

PRODUCTS

 SYBR Green qPCR Master Mix-695100011730

SYBR Green qPCR Master Mix-695100011730

₹ 13,840.00

Brand: Bangalore GeNei

Catalog No.: 695100011730

Quantity/Unit: 200 x 25 µL rxns/Pack

Usually Shipped in: 2-3 Weeks

(STWSSV50) Single Tube WSSV Detection Kit-680300011730

(STWSSV50) Single Tube WSSV Detection Kit-680300011730

₹ 22,770.00

Brand: Bangalore GeNei

Catalog No.: 680300011730

Quantity/Unit: 50 tests/Kit

Usually Shipped in: 2-3 Weeks

(WSS V100A) WSSV Detection Kit-680500051730

(WSS V100A) WSSV Detection Kit-680500051730

₹ 42,640.00

Brand: Bangalore GeNei

Catalog No.: 680500051730

Quantity/Unit: 100 tests/Kit

Usually Shipped in: 2-3 Weeks

10X DreamTaq Buffer

10X DreamTaq Buffer

₹ 2,323.00

Brand: Fermentas

Catalog No.: B65

Quantity/Unit: 4 x 1.25 mL/Pack

Usually Shipped in: 1-2 Weeks

10X DreamTaq Green Buffer

10X DreamTaq Green Buffer

₹ 2,323.00

Brand: Fermentas

Catalog No.: B71

Quantity/Unit: 4 x 1.25 mL/Pack

Usually Shipped in: 1-2 Weeks

10X PBS, pH 7.4-ML023-100ML

10X PBS, pH 7.4-ML023-100ML

₹ 2,954.00

Brand: HIMEDIA

Catalog No.: ML023-100ML

Quantity/Unit: 5X100ml/PACK

Usually Shipped in: 2-3 Weeks

10X Reaction Buffer with MgCl2 for DNase I

10X Reaction Buffer with MgCl2 for DNase I

₹ 1,607.00

Brand: Fermentas

Catalog No.: B43

Quantity/Unit: 1 mL/Vial

Usually Shipped in: 1-2 Weeks

10X TMB Substrate-ML168-5ML

10X TMB Substrate-ML168-5ML

₹ 763.00

Brand: HIMEDIA

Catalog No.: ML168-5ML

Quantity/Unit: 5ml/PACK

Usually Shipped in: 2-3 Weeks

10X Taq Buffer with (NH4)2SO4

10X Taq Buffer with (NH4)2SO4

₹ 2,303.00

Brand: Fermentas

Catalog No.: B33

Quantity/Unit: 4 x 1.25 mL/Pack

Usually Shipped in: 1-2 Weeks

10X Taq Buffer with (NH4)2SO4 and 20 mM MgCl2

10X Taq Buffer with (NH4)2SO4 and 20 mM MgCl2

₹ 2,431.00

Brand: Fermentas

Catalog No.: B34

Quantity/Unit: 4 x 1.25 mL/Pack

Usually Shipped in: 1-2 Weeks

10X Taq Buffer with KCl and 15 mM MgCl2

10X Taq Buffer with KCl and 15 mM MgCl2

₹ 2,417.00

Brand: Fermentas

Catalog No.: B16

Quantity/Unit: 4 x 1.25 mL/Pack

Usually Shipped in: 1-2 Weeks

10X Taq Buffer with KCl, 4 x 1.25 mL

10X Taq Buffer with KCl, 4 x 1.25 mL

₹ 2,223.00

Brand: Fermentas

Catalog No.: B38

Quantity/Unit: 4 x 1.25 mL/Pack

Usually Shipped in: 1-2 Weeks

10X Taq Buffer without Detergent, 4 x 1.25 mL

10X Taq Buffer without Detergent, 4 x 1.25 mL

₹ 1,641.00

Brand: Fermentas

Catalog No.: B55

Quantity/Unit: 4 x 1.25 mL/Pack

Usually Shipped in: 1-2 Weeks

10X Wash Buffer-DS0230-100ML

10X Wash Buffer-DS0230-100ML

₹ 213.00

Brand: HIMEDIA

Catalog No.: DS0230-100ML

Quantity/Unit: 100ml/PACK

Usually Shipped in: 2-3 Weeks

12864 LCD Display PCR Machine

12864 LCD Display PCR Machine

₹ 95,000.00

Brand: BR Biochem Life Sciences

Catalog No.: BRW-K160

Quantity/Unit: 1 EA/Box

Usually Shipped in: 3-4 Weeks