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PCR

PCR

Polymerase chain reaction or PCR is a technique developed by American biochemist Kary Mullis to amplify a DNA of interest. In a conventional PCR reaction, the first step is the heat-denaturation of the double-stranded DNA at 94 to 96C. This is followed by annealing of the specifically designed primer at the target site and then polymerase catalyzed elongation by the addition of the nucleotides. The denaturation takes about 30 secs, the annealing about 20 to 40 secs and the elongation takes several minutes depending upon the number of cycles set in the PCR machine. PCR machine, DNA template, a polymerase enzyme (usually the heat-resistant Taq polymerase), specifically designed forward and reverse primers, the deoxynucleoside triphosphates or dNTPs mixture which will provide the nucleotides for elongation - they act as the building blocks of the new strand, and the buffer solution to maintain the pH, are the key requirements. The reaction is carried out in 0.1 ml / 0.2 ml PCR tubes or specially designed PCR plates. PCR Kits are also available with everything readymade and quick to use.


In addition to the conventional PCR, a gradient PCR with a range of variation in annealing temperature can also be used. It is Ideal when while developing the protocol for a new DNA sample. Quantitative PCR (qPCR) which uses fluorescent dyes like Sybr Green can be performed to measure the quantity of the amplified products in real-time.

InoMag PCR plates non Skited 0.2ml
₹ 1,300.00
  • Brand: ILS
  • Catalog No.: 8007
  • Quantity/Unit: 10 piece/Pack
  • Usually Shipped in: 4-7 Days
Water, Sterile, Molecular Biology Grade DEPC treated - 20 x 100 ml
₹ 5,050.00
  • Brand: HIMEDIA
  • Catalog No.: TCL018-20X100ML
  • Quantity/Unit: 20X100ml/EA
  • Usually Shipped in: 2-3 Weeks
Water, Sterile, Molecular Biology Grade DEPC treated - 500ml
₹ 1,190.00
  • Brand: HIMEDIA
  • Catalog No.: TCL018-500ML
  • Quantity/Unit: 500ml/PACK
  • Usually Shipped in: 2-3 Weeks
Water, Sterile, Molecular Biology Grade DEPC treated - 10 x 1000 ml
₹ 11,575.00
  • Brand: HIMEDIA
  • Catalog No.: TCL018-10x1000ml
  • Quantity/Unit: 10x1000ml/PACK
  • Usually Shipped in: 2-3 Weeks
Polypropylene sealing film-PR21-1X100No
₹ 8,270.00
  • Brand: HIMEDIA
  • Catalog No.: PR21-1X100No
  • Quantity/Unit: 1x100 No/PACK
  • Usually Shipped in: 2-3 Weeks
Tween 20-MB067-100ML
₹ 4,005.00
  • Brand: HIMEDIA
  • Catalog No.: MB067-100ML
  • Quantity/Unit: 100ml/PACK
  • Usually Shipped in: 2-3 Weeks
ELISA Plates-EP1-5x10NO
₹ 6,005.00
  • Brand: HIMEDIA
  • Catalog No.: EP1-5x10NO
  • Quantity/Unit: 5x10 no/Pack
  • Usually Shipped in: 2-3 Weeks
ELISA Plates-EP1-10x10NO
₹ 11,125.00
  • Brand: HIMEDIA
  • Catalog No.: EP1-10x10NO
  • Quantity/Unit: 10x10 no/Pack
  • Usually Shipped in: 2-3 Weeks
ELISA Plates-EP1-20x10NO
₹ 20,600.00
  • Brand: HIMEDIA
  • Catalog No.: EP1-20x10NO
  • Quantity/Unit: 20x10 no/Pack
  • Usually Shipped in: 2-3 Weeks
ELISA plates-EP2-5X10No
₹ 7,270.00
  • Brand: HIMEDIA
  • Catalog No.: EP2-5X10No
  • Quantity/Unit: 5x10No/PACK
  • Usually Shipped in: 2-3 Weeks
ELISA plates-EP2-10X10No
₹ 13,465.00
  • Brand: HIMEDIA
  • Catalog No.: EP2-10X10No
  • Quantity/Unit: 10x10 No/PACK
  • Usually Shipped in: 2-3 Weeks
PCR Plates Family, PP, Capacity-96 Well, unskirted elevated top standard plate
₹ 2,021.40
  • Brand: ABDOS
  • Catalog No.: P10715
  • Quantity/Unit: 10/Case
  • Usually Shipped in: 1-2 Weeks
PCR Plates Family, PP, Capacity-384 Well fully skirted well
₹ 3,613.10
  • Brand: ABDOS
  • Catalog No.: P10717
  • Quantity/Unit: 10/Case
  • Usually Shipped in: 1-2 Weeks
PCR Tubes, PP, Capacity-0.2 ml, Type-Domed Cap, Height
₹ 2,961.00
  • Brand: ABDOS
  • Catalog No.: P10702
  • Quantity/Unit: 1000/Case
  • Usually Shipped in: 1-2 Weeks
PCR Workup Rack with Lid, PP, Tube Capacity-1 ml
₹ 2,200.00
  • Brand: ABDOS
  • Catalog No.: P11101
  • Quantity/Unit: 5/Case
  • Usually Shipped in: 1-2 Weeks
Aluminium Plate Seal- 157200
  • Brand: TARSONS
  • Catalog No.: 157200
  • Quantity/Unit: 100/Pack
  • Usually Shipped in: 1-2 Weeks
cDNA Synthesis Kit- MPC-2
₹ 7,500.00
  • Brand: Aura Biotech
  • Catalog No.: MPC-2
  • Quantity/Unit: 50 Rxns/Pack
  • Usually Shipped in: 1-3 Days
cDNA Synthesis Kit- MPC-3
₹ 13,000.00
  • Brand: Aura Biotech
  • Catalog No.: MPC-3
  • Quantity/Unit: 100 Rxns/Pack
  • Usually Shipped in: 1-3 Days
cDNA Synthesis Kit- MPC-4
₹ 18,500.00
  • Brand: Aura Biotech
  • Catalog No.: MPC-4
  • Quantity/Unit: 200 Rxns/Pack
  • Usually Shipped in: 1-3 Days
PCR Teaching Kit- 10 reactions
₹ 4,100.00
  • Brand: Aura Biotech
  • Catalog No.: MPL-01S10rxn
  • Quantity/Unit: 10 Rxns/Pack
  • Usually Shipped in: 1-3 Days
PCR Anti-inhibitor ,100 reactions
₹ 3,748.50
  • Brand: Blirt
  • Catalog No.: RP50
  • Quantity/Unit: 100 reactions/pack
PCR Anti-inhibitor ,500 reactions
₹ 15,975.75
  • Brand: Blirt
  • Catalog No.: RP51
  • Quantity/Unit: 500 reactions/pack
T4 DNA Ligase,500 U
₹ 4,373.25
  • Brand: Blirt
  • Catalog No.: EN11-050
  • Quantity/Unit: 500 U/bottle
T4 DNA Ligase,2500 U
₹ 14,904.75
  • Brand: Blirt
  • Catalog No.: EN11-250
  • Quantity/Unit: 2500 U/bottle
Quick Ligase ,50 reactions
₹ 4,194.75
  • Brand: Blirt
  • Catalog No.: EN12-050
  • Quantity/Unit: 50 reactions/bottle
Quick Ligase ,150 reactions
₹ 11,424.00
  • Brand: Blirt
  • Catalog No.: EN12-150
  • Quantity/Unit: 150 reactions/bottle
Tth DNA Ligase ,250 U
₹ 3,927.00
  • Brand: Blirt
  • Catalog No.: EN13-025
  • Quantity/Unit: 250 U/bottle
Tth DNA Ligase ,2500 U
₹ 26,328.75
  • Brand: Blirt
  • Catalog No.: EN13-250
  • Quantity/Unit: 2500 U/bottle