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PCR

PCR

Polymerase chain reaction or PCR is a technique developed by American biochemist Kary Mullis to amplify a DNA of interest. In a conventional PCR reaction, the first step is the heat-denaturation of the double-stranded DNA at 94 to 96C. This is followed by annealing of the specifically designed primer at the target site and then polymerase catalyzed elongation by the addition of the nucleotides. The denaturation takes about 30 secs, the annealing about 20 to 40 secs and the elongation takes several minutes depending upon the number of cycles set in the PCR machine. PCR machine, DNA template, a polymerase enzyme (usually the heat-resistant Taq polymerase), specifically designed forward and reverse primers, the deoxynucleoside triphosphates or dNTPs mixture which will provide the nucleotides for elongation - they act as the building blocks of the new strand, and the buffer solution to maintain the pH, are the key requirements. The reaction is carried out in 0.1 ml / 0.2 ml PCR tubes or specially designed PCR plates. PCR Kits are also available with everything readymade and quick to use.


In addition to the conventional PCR, a gradient PCR with a range of variation in annealing temperature can also be used. It is Ideal when while developing the protocol for a new DNA sample. Quantitative PCR (qPCR) which uses fluorescent dyes like Sybr Green can be performed to measure the quantity of the amplified products in real-time.

5 LAMP buffer, 5 1.25 ml

Brand: SynGen

₹ 2,250.00
  • Brand: SynGen
  • Catalog No.: BS00005
  • Quantity/Unit: 5 ? 1.25 ml/Pack
5 PCR buffer (0m Mg2+), 5 1.25 ml

Brand: SynGen

₹ 2,250.00
  • Brand: SynGen
  • Catalog No.: BM00002
  • Quantity/Unit: 5 ? 1.25 ml/Pack
5 PCR buffer (0m Mg2+), 2 1.25 ml

Brand: SynGen

₹ 961.00
  • Brand: SynGen
  • Catalog No.: BS00002
  • Quantity/Unit: 2 ? 1.25 ml/Pack
RT buffer, 5 1.25 ml

Brand: SynGen

₹ 2,250.00
  • Brand: SynGen
  • Catalog No.: BS00004
  • Quantity/Unit: 5 ? 1.25 ml/Pack
Pol buffer, 5 1.25 ml

Brand: SynGen

₹ 2,250.00
  • Brand: SynGen
  • Catalog No.: BS00007
  • Quantity/Unit: 5 ? 1.25 ml/Pack
5 PCR buffer, 5 1.25 ml

Brand: SynGen

₹ 2,250.00
  • Brand: SynGen
  • Catalog No.: BM00001
  • Quantity/Unit: 5 ? 1.25 ml/Pack
5 PCR buffer, 2 1.25 ml

Brand: SynGen

₹ 961.00
  • Brand: SynGen
  • Catalog No.: BS00001
  • Quantity/Unit: 2 ? 1.25 ml/Pack
Synta-ExoP mix, 2000 rxns

Brand: SynGen

₹ 1,57,500.00
  • Brand: SynGen
  • Catalog No.: MM00005
  • Quantity/Unit: 2000 rxns/Pack
Synta-ExoP mix, 500 rxns

Brand: SynGen

₹ 45,000.00
  • Brand: SynGen
  • Catalog No.: MS00005
  • Quantity/Unit: 500 rxns/Pack
Synta-Ab Taq DNA-polymerase, 5000 units/ml

Brand: SynGen

₹ 34,200.00
  • Brand: SynGen
  • Catalog No.: EM00003
  • Quantity/Unit: 5000 units/Pack
Synta-Ab Taq DNA-polymerase, 1000 units/ml

Brand: SynGen

₹ 8,550.00
  • Brand: SynGen
  • Catalog No.: ES00003
  • Quantity/Unit: 1000 units/Pack
Synta-F Taq DNA-polymerase, 5000 units/ml

Brand: SynGen

₹ 32,400.00
  • Brand: SynGen
  • Catalog No.: EM00002
  • Quantity/Unit: 5000 units/Pack
Water for PCR, 2 1.25 ml

Brand: SynGen

₹ 720.00
  • Brand: SynGen
  • Catalog No.: RS00002
  • Quantity/Unit: 2 ? 1.25 ml/Pack
Synta-F Taq DNA-polymerase, 1000 units/ml

Brand: SynGen

₹ 7,020.00
  • Brand: SynGen
  • Catalog No.: ES00002
  • Quantity/Unit: 1000 units/Pack
MgSO4 100 m, 5 1.0 ml

Brand: SynGen

₹ 4,500.00
  • Brand: SynGen
  • Catalog No.: RM00001
  • Quantity/Unit: 5 ? 1.0 ml/Pack
MgSO4 100 m, 1 1.0 ml

Brand: SynGen

₹ 1,385.00
  • Brand: SynGen
  • Catalog No.: RS00001
  • Quantity/Unit: 1 ? 1.0 ml/Pack
Synta Taq DNA-polymerase, 5000 units/ml

Brand: SynGen

₹ 8,100.00
  • Brand: SynGen
  • Catalog No.: EM00001
  • Quantity/Unit: 5000 units/Pack
Synta Taq DNA-polymerase, 1000 units/ml

Brand: SynGen

₹ 2,520.00
  • Brand: SynGen
  • Catalog No.: ES00001
  • Quantity/Unit: 1000 units/Pack
5 Synta-F PCR Master Mix, 2500 rxns

Brand: SynGen

₹ 90,000.00
  • Brand: SynGen
  • Catalog No.: MM00001
  • Quantity/Unit: 2500 rxns/Pack
5 Synta-F PCR Master Mix, 1000 rxns

Brand: SynGen

₹ 38,572.00
  • Brand: SynGen
  • Catalog No.: MS00001
  • Quantity/Unit: 1000 rxns/Pack
5 Synta-Ab RT-PCR Master Mix, 2500 rxns

Brand: SynGen

₹ 3,06,000.00
  • Brand: SynGen
  • Catalog No.: MM00004
  • Quantity/Unit: 2500 rxns/Pack
5 Synta-Ab RT-PCR Master Mix, 1000 rxns

Brand: SynGen

₹ 1,36,153.00
  • Brand: SynGen
  • Catalog No.: MS00004
  • Quantity/Unit: 1000 rxns/Pack
5 Synta-F RT-PCR Master Mix, 2500 rxns

Brand: SynGen

₹ 2,88,000.00
  • Brand: SynGen
  • Catalog No.: MM00003
  • Quantity/Unit: 2500 rxns/Pack
5 Synta-F RT-PCR Master Mix, 1000 rxns

Brand: SynGen

₹ 1,28,000.00
  • Brand: SynGen
  • Catalog No.: MS00003
  • Quantity/Unit: 1000 rxns/Pack
5 Synta-Ab PCR Master Mix, 2500 rxns

Brand: SynGen

₹ 1,35,000.00
  • Brand: SynGen
  • Catalog No.: MM00002
  • Quantity/Unit: 2500 rxns/Pack
5 Synta-Ab PCR Master Mix, 1000 rxns

Brand: SynGen

₹ 54,000.00
  • Brand: SynGen
  • Catalog No.: MS00002
  • Quantity/Unit: 1000 rxns/Pack
Synta-Bst L DNA-polymerase, 1600 units/ml

Brand: SynGen

₹ 11,466.00
  • Brand: SynGen
  • Catalog No.: ES00008
  • Quantity/Unit: 1600 units/Pack
Synta-Bst L DNA-polymerase, 8000 units/ml

Brand: SynGen

₹ 44,100.00
  • Brand: SynGen
  • Catalog No.: EM00008
  • Quantity/Unit: 8000 units/Pack