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Protein Purification
Protein purification is the process of extraction of proteins while excluding cellular components and other contaminants. The purification processes may involve several different principles suchas differences in protein size,physicochemicalproperties, binding affinity and biological activity. Initially, the extraction process may be carried out by repeated freezing and thawing, homogenization by high pressure, lysis using detergents or enzymes, or other techniques. Following this, the cell debris is separated from the proteins and other soluble cell components by centrifugation.
There are several different purification processes and techniques available to work with proteins. Initial steps include precipitation using agents such as ammoniumsulphate, using detergents to dissolve cell membrane proteins. For the actualpurification,there are several chromatographic techniques available such as size exclusion, ion-exchange, affinity chromatography, HPLC and so forth.
There are various chemical reagents required for purification procedures which are conveniently bundled in kits etc. On Biomall, these are available from popular brands that include Pierce, BD Biosciences, HiMedia and several more.
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AminoLink Coupling Resin
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Quantity/Unit: 10 ML/pack
Usually Shipped in: 3-4 Weeks
AminoLink Plus Immobilization Trial Kit 2ml
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Quantity/Unit: 1 column/pack
Usually Shipped in: 3-4 Weeks
AminoLink Reductant
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Quantity/Unit: 2 X 1 G/pack
Usually Shipped in: 3-4 Weeks
Ammonium Persulfate
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Quantity/Unit: 4x25 gm/Vial
Usually Shipped in: 3-4 Weeks
CD38
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Quantity/Unit: 100 Tests/Kit
Usually Shipped in: 3-4 Weeks
CD79b
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Quantity/Unit: 100 Tests/Kit
Usually Shipped in: 3-4 Weeks
Cycletest Plus DNA Reagent Kit
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Quantity/Unit: 40 Tests/Kit
Usually Shipped in: 3-4 Weeks
DTT(1,4-Dithiothreitol) 2g
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Quantity/Unit: 2 gm/Pack
Usually Shipped in: 2-3 Weeks
E-64 Protease Inhibitor
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Quantity/Unit: 10 MG/pack
Usually Shipped in: 3-4 Weeks
Float Buoys for 0.1mL Slide-A-Lyzer
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Quantity/Unit: 1X4/pack
Usually Shipped in: 3-4 Weeks
GST - Fusion Protein Purification Kit-2160600011730
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Quantity/Unit: 5 preps/Kit
Usually Shipped in: 2-3 Weeks
GST Protein Interaction Pull-Down Kit
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Quantity/Unit: 25 rxns kit/pack
Usually Shipped in: 3-4 Weeks
GST-Tagged Bacterial Protein Purification Kit (Gravity Flow)
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Quantity/Unit: 5no/EA
Usually Shipped in: 2-3 Weeks
GST-Tagged Bacterial Protein Purification Kit (Gravity Flow)-MBPP003-2NO
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Quantity/Unit: 2no/EA
Usually Shipped in: 2-3 Weeks
GST-Tagged Bacterial Protein Purification Kit (Spin Column)
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Quantity/Unit: 25no/EA
Usually Shipped in: 2-3 Weeks
