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Protein Purification

Protein Purification

Protein purification is the process of extraction of proteins while excluding cellular components and other contaminants. The purification processes may involve several different principles suchas differences in protein size,physicochemicalproperties, binding affinity and biological activity. Initially, the extraction process may be carried out by repeated freezing and thawing, homogenization by high pressure, lysis using detergents or enzymes, or other techniques. Following this, the cell debris is separated from the proteins and other soluble cell components by centrifugation.

There are several different purification processes and techniques available to work with proteins. Initial steps include precipitation using agents such as ammoniumsulphate, using detergents to dissolve cell membrane proteins. For the actualpurification,there are several chromatographic techniques available such as size exclusion, ion-exchange, affinity chromatography, HPLC and so forth.

There are various chemical reagents required for purification procedures which are conveniently bundled in kits etc. On Biomall, these are available from popular brands that include Pierce, BD Biosciences, HiMedia and several more.

His - Tag Fusion Protein Purification Kit-2160700011730
₹ 12,991.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2160700011730
  • Quantity/Unit: 5 preps/Kit
IgG Purification Kit-2160100021730
₹ 15,345.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2160100021730
  • Quantity/Unit: 1 Kit/Kit
Monoclonal IgG Purification Kit-2160500011730
₹ 35,068.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2160500011730
  • Quantity/Unit: 3 preps/Kit
Protein A Immunoprecipitation Kit-1660400011730
₹ 13,134.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1660400011730
  • Quantity/Unit: 20 preps/Kit
Purification of His Tag fusion protein (NTA based) Kit-2160900011730
₹ 17,897.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2160900011730
  • Quantity/Unit: 4 preps/Kit
Immunoglobulin Purification Kit-2160300011730
₹ 11,715.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2160300011730
  • Quantity/Unit: 3 preps/Kit
GST - Fusion Protein Purification Kit-2160600011730
₹ 12,881.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2160600011730
  • Quantity/Unit: 5 preps/Kit
DTT(1,4-Dithiothreitol) 2g
₹ 3,795.00
  • Brand: Bangalore GeNei
  • Catalog No.: 3601000021730
  • Quantity/Unit: 2 gm/Pack
PureCube 100 Ni-NTA Agarose resins High Affinity Chromatography
₹ 2,100.00 ₹ 4,200.00
50% Off
  • Brand: Cube Biotech
  • Catalog No.: 74101
  • Quantity/Unit: 1 ml/vial
  • Usually Shipped in: 1-3 Days
PureCube Ni-NTA Agarose resins for High Affinity Chromatography
₹ 2,000.00 ₹ 4,000.00
50% Off
  • Brand: Cube Biotech
  • Catalog No.: 31101
  • Quantity/Unit: 1 ml/vial
  • Usually Shipped in: 1-3 Days
PureCube 100 INDIGO Ni-Agarose resins for High Affinity Chromatography
₹ 2,100.00 ₹ 4,200.00
50% Off
  • Brand: Cube Biotech
  • Catalog No.: 75101
  • Quantity/Unit: 1 ml/vial
  • Usually Shipped in: 1-3 Days
PureCube Glutathione Agarose resins for High Affinity Chromatography
₹ 2,850.00 ₹ 5,700.00
50% Off
  • Brand: Cube Biotech
  • Catalog No.: 32101
  • Quantity/Unit: 1 ml/vial
  • Usually Shipped in: 1-3 Days
GENELUTE (TM) RNA/DNA/PROTEIN PURIFICATION
₹ 40,812.66 ₹ 58,303.79
30% Off
  • Brand: Sigma-Aldrich
  • Catalog No.: RDP300-50RXN
  • Quantity/Unit: 50RXN/Pack
  • Usually Shipped in: 4-7 Days
Protein Crystallization Kit
₹ 19,650.00
  • Brand: HIMEDIA
  • Catalog No.: MBPC001-1PK
  • Quantity/Unit: 1pack/EA
  • Usually Shipped in: 2-3 Weeks
IgG Purification Kit (Protein A Based, Gravity Flow)-MBPP001-2NO
₹ 17,515.00
  • Brand: HIMEDIA
  • Catalog No.: MBPP001-2NO
  • Quantity/Unit: 2no/EA
  • Usually Shipped in: 2-3 Weeks
IgG Purification Kit (Protein A Based, Gravity Flow)
₹ 41,540.00
  • Brand: HIMEDIA
  • Catalog No.: MBPP001-5NO
  • Quantity/Unit: 5no/EA
  • Usually Shipped in: 2-3 Weeks
IgG Purification Kit (Protein A Based, Spin Column)-MBPP001SP-5NO
₹ 4,155.00
  • Brand: HIMEDIA
  • Catalog No.: MBPP001SP-5NO
  • Quantity/Unit: 5no/EA
  • Usually Shipped in: 2-3 Weeks
IgG Purification Kit (Protein A Based, Spin Column)
₹ 20,435.00
  • Brand: HIMEDIA
  • Catalog No.: MBPP001SP-25NO
  • Quantity/Unit: 25no/EA
  • Usually Shipped in: 2-3 Weeks
IgG Purification Kit (Protein G Based, Gravity Flow)-MBPP002-2NO
₹ 18,320.00
  • Brand: HIMEDIA
  • Catalog No.: MBPP002-2NO
  • Quantity/Unit: 2no/EA
  • Usually Shipped in: 2-3 Weeks
IgG Purification Kit (Protein G Based, Gravity Flow)
₹ 43,620.00
  • Brand: HIMEDIA
  • Catalog No.: MBPP002-5NO
  • Quantity/Unit: 5no/EA
  • Usually Shipped in: 2-3 Weeks
IgG Purification Kit (Protein G Based, Spin Column)-MBPP002SP-5NO
₹ 4,380.00
  • Brand: HIMEDIA
  • Catalog No.: MBPP002SP-5NO
  • Quantity/Unit: 5no/EA
  • Usually Shipped in: 2-3 Weeks
IgG Purification Kit (Protein G Based, Spin Column)
₹ 21,220.00
  • Brand: HIMEDIA
  • Catalog No.: MBPP002SP-25NO
  • Quantity/Unit: 25no/EA
  • Usually Shipped in: 2-3 Weeks
IgG Purification Kit (Protein L Based, Gravity Flow)-MBPP004-2NO
₹ 55,010.00
  • Brand: HIMEDIA
  • Catalog No.: MBPP004-2NO
  • Quantity/Unit: 2no/EA
  • Usually Shipped in: 2-3 Weeks
IgG Purification Kit (Protein L Based, Gravity Flow)
₹ 1,21,030.00
  • Brand: HIMEDIA
  • Catalog No.: MBPP004-5NO
  • Quantity/Unit: 5no/EA
  • Usually Shipped in: 2-3 Weeks
IgG Purification Kit (Protein L Based, Spin Column)-MBPP004SP-5NO
₹ 8,815.00
  • Brand: HIMEDIA
  • Catalog No.: MBPP004SP-5NO
  • Quantity/Unit: 5no/EA
  • Usually Shipped in: 2-3 Weeks
IgG Purification Kit (Protein L Based, Spin Column)
₹ 39,690.00
  • Brand: HIMEDIA
  • Catalog No.: MBPP004SP-25NO
  • Quantity/Unit: 25no/EA
  • Usually Shipped in: 2-3 Weeks
GST-Tagged Bacterial Protein Purification Kit (Gravity Flow)-MBPP003-2NO
₹ 11,845.00
  • Brand: HIMEDIA
  • Catalog No.: MBPP003-2NO
  • Quantity/Unit: 2no/EA
  • Usually Shipped in: 2-3 Weeks
GST-Tagged Bacterial Protein Purification Kit (Gravity Flow)
₹ 26,935.00
  • Brand: HIMEDIA
  • Catalog No.: MBPP003-5NO
  • Quantity/Unit: 5no/EA
  • Usually Shipped in: 2-3 Weeks