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Western Blotting

Western Blotting

Western Blotting, also known as immunoblotting, is a widely used method/technique to detect and/or quantify specific proteins from the sample of interest. The procedure involves a few stages. The protein sample is initially loaded and run on polyacrylamide gel electrophoresis wherein a voltage is applied and the protein separates based on their charge/mass ratio. A reference protein ladder is used to assess the molecular weight of proteins in the sample. The proteins are then transferred from the gel onto a membrane usually made of nitrocellulose or polyvinylidene difluoride (PVDF). This transfer is accomplished by electric current in electroblotting or by capillary action through the filter papers. A primary antibody binds to the protein of interest (now on the membrane). In some procedures, a secondary antibody is also used which binds to the primary antibody's Fc region. Various methods can be used for final detection of antibody bound protein of interest like Chemiluminescent detection or Fluorescent detection depending on the probe.
Western Blotting is a highly sensitive method for protein detection. In diagnostics, it is employed in detecting anti-HIV antibody and Hepatitis B infection. In molecular biology, it can detect the presence of phosphorylated proteins, tagged proteins or various isoforms of proteins. In genetics, it can be used for gene expression studies amongst others.
GeNei Southern Hybridization Teaching Kit-6109600011730
₹ 11,242.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6109600011730
  • Quantity/Unit: 5 expts./Kit
GeNei Southern Hybridization Teaching Kit with ETS5-6109600031730
₹ 35,222.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6109600031730
  • Quantity/Unit: 5 expts./Kit
GeNei Northern Blotting Teaching Kit-6113800011730
₹ 15,169.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6113800011730
  • Quantity/Unit: 5 expts./Kit
GeNei Western Blotting Teaching Kit-6102100011730
₹ 13,915.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6102100011730
  • Quantity/Unit: 5 expts./Kit
GeNei Western Blotting Teaching Kit-6102100021730
₹ 32,010.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6102100021730
  • Quantity/Unit: 20 expts./Kit
GeNei Western Blotting Teaching Kit with ETS3-6102100031730
₹ 45,100.00
  • Brand: Bangalore GeNei
  • Catalog No.: 6102100031730
  • Quantity/Unit: 5 expts./Kit
DAB System-1610500011730
₹ 6,380.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1610500011730
  • Quantity/Unit: 10x6mg/Pack
TMB/H2O2 for localization-1610380101730
₹ 3,861.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1610380101730
  • Quantity/Unit: 10 ml/Pack
BCIP/NBT-1610280501730
₹ 3,949.00
  • Brand: Bangalore GeNei
  • Catalog No.: 1610280501730
  • Quantity/Unit: 50 ml/Pack
Western Blot Development Kit for Human Antibody-2601300011730
₹ 14,234.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2601300011730
  • Quantity/Unit: 10 blots/Kit
Western Blot Development Kit for Mouse Antibody-2601400011730
₹ 14,234.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2601400011730
  • Quantity/Unit: 10 blots/Kit
Western Blot Development Kit for Rabbit Antibody-2601500011730
₹ 14,234.00
  • Brand: Bangalore GeNei
  • Catalog No.: 2601500011730
  • Quantity/Unit: 10 blots/Kit
Nitrocellulose Blotting Membrane Roll, 30 cm X 3 m, 0.22 um-SF110A-1ROLL
₹ 8,905.00
  • Brand: HIMEDIA
  • Catalog No.: SF110A-1ROLL
  • Quantity/Unit: 1 roll/PACK
  • Usually Shipped in: 2-3 Weeks
Nitrocellulose Blotting Membrane Roll, 30 cm X 3 m, 0.45 um-SF110B-1ROLL
₹ 8,905.00
  • Brand: HIMEDIA
  • Catalog No.: SF110B-1ROLL
  • Quantity/Unit: 1 roll/PACK
  • Usually Shipped in: 2-3 Weeks
Nylon-66 Blotting Membrane Roll, 30 cm X 3 m, 0.22 um-SF114A-1ROLL
₹ 14,365.00
  • Brand: HIMEDIA
  • Catalog No.: SF114A-1ROLL
  • Quantity/Unit: 1 roll/PACK
  • Usually Shipped in: 2-3 Weeks
Nylon-66 Blotting Membrane Roll, 30 cm X 3 m, 0.45 um-SF114B-1ROLL
₹ 14,365.00
  • Brand: HIMEDIA
  • Catalog No.: SF114B-1ROLL
  • Quantity/Unit: 1 roll/PACK
  • Usually Shipped in: 2-3 Weeks
Nitrocellulose Blotting Membrane, 15 X 15 cm, 0.22 um-SF107A-1x25NO
₹ 5,935.00
  • Brand: HIMEDIA
  • Catalog No.: SF107A-1x25NO
  • Quantity/Unit: 1x25 no/PACK
  • Usually Shipped in: 2-3 Weeks
Nitrocellulose Blotting Membrane, 15 X 15 cm, 0.45 um-SF107B-1x25NO
₹ 5,935.00
  • Brand: HIMEDIA
  • Catalog No.: SF107B-1x25NO
  • Quantity/Unit: 1x25 no/PACK
  • Usually Shipped in: 2-3 Weeks
Nitrocellulose Blotting Membrane, 20 X 20 cm, 0.22 um-SF108A-1x25NO
₹ 9,575.00
  • Brand: HIMEDIA
  • Catalog No.: SF108A-1x25NO
  • Quantity/Unit: 1x25 no/PACK
  • Usually Shipped in: 2-3 Weeks
Nitrocellulose Blotting Membrane, 20 X 20 cm,0.45 um-SF108B-1x25NO
₹ 9,575.00
  • Brand: HIMEDIA
  • Catalog No.: SF108B-1x25NO
  • Quantity/Unit: 1x25 no/PACK
  • Usually Shipped in: 2-3 Weeks
Nitrocellulose Blotting Membrane, 30 X 30 cm, 0.22 um-SF109A-1X25NO
₹ 14,365.00
  • Brand: HIMEDIA
  • Catalog No.: SF109A-1X25NO
  • Quantity/Unit: 1x25 no/PACK
  • Usually Shipped in: 2-3 Weeks
Nitrocellulose Blotting Membrane, 30 X 30 cm, 0.45 um-SF109B-1X25NO
₹ 14,365.00
  • Brand: HIMEDIA
  • Catalog No.: SF109B-1X25NO
  • Quantity/Unit: 1x25 no/PACK
  • Usually Shipped in: 2-3 Weeks
Nylon-66 Blotting Membrane, 15 X 15 cm, 0.22 um-SF111A-1x25NO
₹ 10,375.00
  • Brand: HIMEDIA
  • Catalog No.: SF111A-1x25NO
  • Quantity/Unit: 1x25 no/PACK
  • Usually Shipped in: 2-3 Weeks
Nylon-66 Blotting Membrane, 15 X 15 cm, 0.45 um-SF111B-1x25NO
₹ 10,375.00
  • Brand: HIMEDIA
  • Catalog No.: SF111B-1x25NO
  • Quantity/Unit: 1x25 no/PACK
  • Usually Shipped in: 2-3 Weeks
Nylon-66 Blotting Membrane, 20 X 20 cm, 0.22 um-SF112A-1x25NO
₹ 18,675.00
  • Brand: HIMEDIA
  • Catalog No.: SF112A-1x25NO
  • Quantity/Unit: 1x25 no/PACK
  • Usually Shipped in: 2-3 Weeks
Nylon-66 Blotting Membrane, 20 X 20 cm, 0.45 um-SF112B-1x25NO
₹ 18,675.00
  • Brand: HIMEDIA
  • Catalog No.: SF112B-1x25NO
  • Quantity/Unit: 1x25 no/PACK
  • Usually Shipped in: 2-3 Weeks
Nylon-66 Blotting Membrane, 30 X 30 cm, 0.22 um-SF113A-1x25NO
₹ 33,515.00
  • Brand: HIMEDIA
  • Catalog No.: SF113A-1x25NO
  • Quantity/Unit: 1x25 no/PACK
  • Usually Shipped in: 2-3 Weeks
Nylon-66 Blotting Membrane, 30 X 30 cm, 0.45 um-SF113B-1x25NO
₹ 33,515.00
  • Brand: HIMEDIA
  • Catalog No.: SF113B-1x25NO
  • Quantity/Unit: 1x25 no/PACK
  • Usually Shipped in: 2-3 Weeks