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Western Blotting

Western Blotting, also known as immunoblotting, is a widely used method/technique to detect and/or quantify specific proteins from the sample of interest. The procedure involves a few stages. The protein sample is initially loaded and run on polyacrylamide gel electrophoresis wherein a voltage is applied and the protein separates based on their charge/mass ratio. A reference protein ladder is used to assess the molecular weight of proteins in the sample. The proteins are then transferred from the gel onto a membrane usually made of nitrocellulose or polyvinylidene difluoride (PVDF). This transfer is accomplished by electric current in electroblotting or by capillary action through the filter papers. A primary antibody binds to the protein of interest (now on the membrane). In some procedures, a secondary antibody is also used which binds to the primary antibody's Fc region. Various methods can be used for final detection of antibody bound protein of interest like Chemiluminescent detection or Fluorescent detection depending on the probe.
Western Blotting is a highly sensitive method for protein detection. In diagnostics, it is employed in detecting anti-HIV antibody and Hepatitis B infection. In molecular biology, it can detect the presence of phosphorylated proteins, tagged proteins or various isoforms of proteins. In genetics, it can be used for gene expression studies amongst others.
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1-Step TMB-Blotting Substrate Solution

1-Step TMB-Blotting Substrate Solution
₹ 21,620.00

Brand: Pierce Protein Research Products

Catalog No.: PI34018

Quantity/Unit: 250 ML/Bottle

Usually Shipped in: 3-4 Weeks

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1-Step Ultra TMB-Blotting Solution

1-Step Ultra TMB-Blotting Solution
₹ 27,724.00

Brand: Pierce Protein Research Products

Catalog No.: PI37574

Quantity/Unit: 250 ML/Bottle

Usually Shipped in: 3-4 Weeks

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13 x 14 cm P plus Membranes

13 x 14 cm P plus Membranes
₹ 27,724.00

Brand: BioSpan

Catalog No.: 2322452

Quantity/Unit: 10/Pack

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13 x 14 cm Filter Paper for Blotting

13 x 14 cm Filter Paper for Blotting
₹ 27,724.00

Brand: BioSpan

Catalog No.: 2322436

Quantity/Unit: 200 sheets/Box

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20 x 20 cm Filter Paper for Blotting

20 x 20 cm Filter Paper for Blotting
₹ 27,724.00

Brand: BioSpan

Catalog No.: 2392493

Quantity/Unit: 100 sheets/Box

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20 x 20 cm N Membranes

20 x 20 cm N Membranes
₹ 27,724.00

Brand: BioSpan

Catalog No.: 2392394

Quantity/Unit: 10/Pack

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24 x 23 cm Cellophane

24 x 23 cm Cellophane
₹ 27,724.00

Brand: BioSpan

Catalog No.: 2398038

Quantity/Unit: 500/Pack

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26 x 300 cm P plus Membrane

26 x 300 cm P plus Membrane
₹ 27,724.00

Brand: BioSpan

Catalog No.: 2322453

Quantity/Unit: 1 Roll/Pack

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6.5 x 6.5 cm Filter Paper for Blotting

6.5 x 6.5 cm Filter Paper for Blotting
₹ 27,724.00

Brand: BioSpan

Catalog No.: 2322437

Quantity/Unit: 400 sheets/Box

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6.5 x 6.5 cm P plus Membranes

6.5 x 6.5 cm P plus Membranes
₹ 27,724.00

Brand: BioSpan

Catalog No.: 2322450

Quantity/Unit: 20/Pack

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8.5 x 9 cm P plus Membranes

8.5 x 9 cm P plus Membranes
₹ 27,724.00

Brand: BioSpan

Catalog No.: 2322451

Quantity/Unit: 20/Pack

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8.5 x 9 cm Filter Paper for Blotting

8.5 x 9 cm Filter Paper for Blotting
₹ 27,724.00

Brand: BioSpan

Catalog No.: 2392393

Quantity/Unit: 400 sheets/Box

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AE-1460 EzBlot

AE-1460 EzBlot
₹ 27,724.00

Brand: BioSpan

Catalog No.: 2332600

Quantity/Unit: 1 Kit/Box

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AE-1465 EzFastBlot

AE-1465 EzFastBlot
₹ 27,724.00

Brand: BioSpan

Catalog No.: 2332590

Quantity/Unit: 500 mL/Bottle

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AE-1475 EzBlock Chemi

AE-1475 EzBlock Chemi
₹ 27,724.00

Brand: BioSpan

Catalog No.: 2332615

Quantity/Unit: 500 mL /Bottle

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