Western Blotting
Western Blotting, also known as immunoblotting, is a widely used method/technique to detect and/or quantify specific proteins from the sample of interest. The procedure involves a few stages. The protein sample is initially loaded and run on polyacrylamide gel electrophoresis wherein a voltage is applied and the protein separates based on their charge/mass ratio. A reference protein ladder is used to assess the molecular weight of proteins in the sample. The proteins are then transferred from the gel onto a membrane usually made of nitrocellulose or polyvinylidene difluoride (PVDF). This transfer is accomplished by electric current in electroblotting or by capillary action through the filter papers. A primary antibody binds to the protein of interest (now on the membrane). In some procedures, a secondary antibody is also used which binds to the primary antibody's Fc region. Various methods can be used for final detection of antibody bound protein of interest like Chemiluminescent detection or Fluorescent detection depending on the probe.
Western Blotting is a highly sensitive method for protein detection. In diagnostics, it is employed in detecting anti-HIV antibody and Hepatitis B infection. In molecular biology, it can detect the presence of phosphorylated proteins, tagged proteins or various isoforms of proteins. In genetics, it can be used for gene expression studies amongst others.
Microblot - Single cassette western blotting system
₹ 29,900.00
- Brand:
TechnoSource - Catalog No.:
15-00000 - Quantity/Unit: 1 EA/Box
- Usually Shipped in: 5-6 Weeks
Microblot-T - Dual cassette western blotting system
₹ 31,900.00
- Brand:
TechnoSource - Catalog No.:
15-00001 - Quantity/Unit: 1 EA/Box
- Usually Shipped in: 5-6 Weeks
Midiblot-T - Dual cassette western blotting system
₹ 40,200.00
- Brand:
TechnoSource - Catalog No.:
16-00001 - Quantity/Unit: 1 EA/Box
- Usually Shipped in: 5-6 Weeks
Technoblot-O - Single cassette blotting system for western, nothern and Southern blotting
₹ 35,500.00
- Brand:
TechnoSource - Catalog No.:
13-00001 - Quantity/Unit: 1 EA/Box
- Usually Shipped in: 5-6 Weeks
Technoblot - Dual cassette blotting system for western, northern and Southern blotting
₹ 42,500.00
- Brand:
TechnoSource - Catalog No.:
13-00000 - Quantity/Unit: 1 EA/Box
- Usually Shipped in: 5-6 Weeks
Technoblot-F - Four cassette blotting system for western, northern and Southern blotting
₹ 55,250.00
- Brand:
TechnoSource - Catalog No.:
13-00004 - Quantity/Unit: 1 EA/Box
- Usually Shipped in: 5-6 Weeks
Midiblot - Single cassette western blotting system
₹ 36,800.00
- Brand:
TechnoSource - Catalog No.:
16-00000 - Quantity/Unit: 1 EA/Pack
Nanoblot - Single cassette western blotting system
₹ 19,290.00
- Brand:
EcoSep - Catalog No.:
E1100060 - Quantity/Unit: 1 EA/Box
- Usually Shipped in: 2-3 Weeks
Tubbyblot - Single cassette western blotting system
₹ 32,500.00
- Brand:
EcoSep - Catalog No.:
E1300060 - Quantity/Unit: 1 EA/Box
- Usually Shipped in: 2-3 Weeks
DAB System-1610500011730
₹ 6,380.00
- Brand:
Bangalore GeNei - Catalog No.:
1610500011730 - Quantity/Unit: 10x6mg/Pack
- Availability: In stock
TMB/H2O2 for localization-1610380101730
₹ 3,860.00
- Brand:
Bangalore GeNei - Catalog No.:
1610380101730 - Quantity/Unit: 10 ml/Pack
- Availability: In stock
Western Blot Development Kit for Human Antibody-2601300011730
₹ 14,230.00
- Brand:
Bangalore GeNei - Catalog No.:
2601300011730 - Quantity/Unit: 10 blots/Kit
- Availability: In stock
Western Blot Development Kit for Mouse Antibody-2601400011730
₹ 14,230.00
- Brand:
Bangalore GeNei - Catalog No.:
2601400011730 - Quantity/Unit: 10 blots/Kit
- Availability: In stock
Western Blot Development Kit for Rabbit Antibody-2601500011730
₹ 14,230.00
- Brand:
Bangalore GeNei - Catalog No.:
2601500011730 - Quantity/Unit: 10 blots/Kit
- Availability: In stock
GeNei Southern Hybridization Teaching Kit-6109600011730
₹ 11,240.00
- Brand:
Bangalore GeNei - Catalog No.:
6109600011730 - Quantity/Unit: 5 expts./Kit
- Availability: In stock
GeNei Southern Hybridization Teaching Kit with ETS5-6109600031730
₹ 35,220.00
- Brand:
Bangalore GeNei - Catalog No.:
6109600031730 - Quantity/Unit: 5 expts./Kit
- Availability: In stock
GeNei Northern Blotting Teaching Kit-6113800011730
₹ 15,160.00
- Brand:
Bangalore GeNei - Catalog No.:
6113800011730 - Quantity/Unit: 5 expts./Kit
- Availability: In stock
GeNei Western Blotting Teaching Kit-6102100011730
₹ 13,910.00
- Brand:
Bangalore GeNei - Catalog No.:
6102100011730 - Quantity/Unit: 5 expts./Kit
- Availability: In stock
GeNei Western Blotting Teaching Kit-6102100021730
₹ 32,010.00
- Brand:
Bangalore GeNei - Catalog No.:
6102100021730 - Quantity/Unit: 20 expts./Kit
- Availability: In stock
GeNei Western Blotting Teaching Kit with ETS3-6102100031730
₹ 45,090.00
- Brand:
Bangalore GeNei - Catalog No.:
6102100031730 - Quantity/Unit: 5 expts./Kit
- Availability: In stock
BCIP/NBT-1610280501730
₹ 3,940.00
- Brand:
Bangalore GeNei - Catalog No.:
1610280501730 - Quantity/Unit: 50 ml/Pack
- Availability: In stock
Immobilon-PSQ PVDF Membrane
₹ 52,890.01
- Brand:
Sigma-Aldrich - Catalog No.:
ISEQ00010 - Quantity/Unit: 1eA/Pack
- Usually Shipped in: 4-7 Days
Immobilon-E PVDF Membrane
₹ 49,710.00
- Brand:
Sigma-Aldrich - Catalog No.:
IEVH85R - Quantity/Unit: 1EA/Pack
- Usually Shipped in: 4-7 Days
Immobilon NOW Dispenser
₹ 36,689.99
- Brand:
Sigma-Aldrich - Catalog No.:
IMDISP - Quantity/Unit: 1 EA/Pack
- Usually Shipped in: 4-7 Days
Nylon-66 Blotting Membrane, 20 X 20 cm, 0.22 um-SF112A-1x25NO
₹ 20,980.00
- Brand:
HIMEDIA - Catalog No.:
SF112A-1x25NO - Quantity/Unit: 1x25 no/PACK
- Usually Shipped in: 2-3 Weeks
Nylon-66 Blotting Membrane, 20 X 20 cm, 0.45 um-SF112B-1x25NO
₹ 20,980.00
- Brand:
HIMEDIA - Catalog No.:
SF112B-1x25NO - Quantity/Unit: 1x25 no/PACK
- Usually Shipped in: 2-3 Weeks
Nylon-66 Blotting Membrane, 30 X 30 cm, 0.22 um-SF113A-1x25NO
₹ 37,655.00
- Brand:
HIMEDIA - Catalog No.:
SF113A-1x25NO - Quantity/Unit: 1x25 no/PACK
- Usually Shipped in: 2-3 Weeks
Nylon-66 Blotting Membrane, 30 X 30 cm, 0.45 um-SF113B-1x25NO
₹ 37,655.00
- Brand:
HIMEDIA - Catalog No.:
SF113B-1x25NO - Quantity/Unit: 1x25 no/PACK
- Usually Shipped in: 2-3 Weeks