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Western Blotting

Western Blotting, also known as immunoblotting, is a widely used method/technique to detect and/or quantify specific proteins from the sample of interest. The procedure involves a few stages. The protein sample is initially loaded and run on polyacrylamide gel electrophoresis wherein a voltage is applied and the protein separates based on their charge/mass ratio. A reference protein ladder is used to assess the molecular weight of proteins in the sample. The proteins are then transferred from the gel onto a membrane usually made of nitrocellulose or polyvinylidene difluoride (PVDF). This transfer is accomplished by electric current in electroblotting or by capillary action through the filter papers. A primary antibody binds to the protein of interest (now on the membrane). In some procedures, a secondary antibody is also used which binds to the primary antibody's Fc region. Various methods can be used for final detection of antibody bound protein of interest like Chemiluminescent detection or Fluorescent detection depending on the probe.
Western Blotting is a highly sensitive method for protein detection. In diagnostics, it is employed in detecting anti-HIV antibody and Hepatitis B infection. In molecular biology, it can detect the presence of phosphorylated proteins, tagged proteins or various isoforms of proteins. In genetics, it can be used for gene expression studies amongst others.

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1-Step TMB-Blotting Substrate Solution

1-Step TMB-Blotting Substrate Solution
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Brand: Pierce Protein Research Products

Catalog No.: PI34018

Quantity/Unit: 250 ML/Bottle

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1-Step Ultra TMB-Blotting Solution

1-Step Ultra TMB-Blotting Solution
₹ 30,496.00

Brand: Pierce Protein Research Products

Catalog No.: PI37574

Quantity/Unit: 250 ML/Bottle

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Amino Acid Standard H

Amino Acid Standard H
₹ 20,997.00

Brand: Pierce Protein Research Products

Catalog No.: PI20088

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BCIP-T, 1 gram

BCIP-T, 1 gram

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Catalog No.: R0821

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BCIP/NBT-1610280501730

BCIP/NBT-1610280501730
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Biodyne B Nylon Membrane, 0.45m, 8cm x 12cm

Biodyne B Nylon Membrane, 0.45m, 8cm x 12cm
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Brand: Pierce Protein Research Products

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DAB System-1610500011730

DAB System-1610500011730
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Fast Western Blot Kits, SuperSignal West Dura, Mouse-PI35070

Fast Western Blot Kits, SuperSignal West Dura, Mouse-PI35070
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Brand: Pierce Protein Research Products

Catalog No.: PI35070

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Fast Western Blot Kits, SuperSignal West Dura, Rabbit-PI35071

Fast Western Blot Kits, SuperSignal West Dura, Rabbit-PI35071
₹ 72,999.00

Brand: Pierce Protein Research Products

Catalog No.: PI35071

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Fast Western Blot Kits, SuperSignal West Femto, Mouse-PI35080

Fast Western Blot Kits, SuperSignal West Femto, Mouse-PI35080
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Brand: Pierce Protein Research Products

Catalog No.: PI35080

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Fast Western Blot Kits, SuperSignal West Femto, Rabbit-PI35081

Fast Western Blot Kits, SuperSignal West Femto, Rabbit-PI35081
₹ 77,163.00

Brand: Pierce Protein Research Products

Catalog No.: PI35081

Quantity/Unit: 400 ml/Kit

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Fast Western Kit, SuperSignal West Pico, Mouse

Fast Western Kit, SuperSignal West Pico, Mouse
₹ 21,441.00

Brand: Pierce Protein Research Products

Catalog No.: PI35065

Quantity/Unit: 200 ml/Kit

Usually Shipped in: 3-4 Weeks

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Fast Western Kit, SuperSignal West Pico, Mouse-PI35060

Fast Western Kit, SuperSignal West Pico, Mouse-PI35060
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Brand: Pierce Protein Research Products

Catalog No.: PI35060

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Fast Western Kit, SuperSignal West Pico, Rabbit

Fast Western Kit, SuperSignal West Pico, Rabbit
₹ 22,325.00

Brand: Pierce Protein Research Products

Catalog No.: PI35066

Quantity/Unit: 200 ml/Kit

Usually Shipped in: 3-4 Weeks

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Fast Western Kit, SuperSignal West Pico, Rabbit-PI35061

Fast Western Kit, SuperSignal West Pico, Rabbit-PI35061
₹ 43,211.00

Brand: Pierce Protein Research Products

Catalog No.: PI35061

Quantity/Unit: 800 ml/Pack

Usually Shipped in: 3-4 Weeks

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