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Western Blotting

Western Blotting, also known as immunoblotting, is a widely used method/technique to detect and/or quantify specific proteins from the sample of interest. The procedure involves a few stages. The protein sample is initially loaded and run on polyacrylamide gel electrophoresis wherein a voltage is applied and the protein separates based on their charge/mass ratio. A reference protein ladder is used to assess the molecular weight of proteins in the sample. The proteins are then transferred from the gel onto a membrane usually made of nitrocellulose or polyvinylidene difluoride (PVDF). This transfer is accomplished by electric current in electroblotting or by capillary action through the filter papers. A primary antibody binds to the protein of interest (now on the membrane). In some procedures, a secondary antibody is also used which binds to the primary antibody's Fc region. Various methods can be used for final detection of antibody bound protein of interest like Chemiluminescent detection or Fluorescent detection depending on the probe.
Western Blotting is a highly sensitive method for protein detection. In diagnostics, it is employed in detecting anti-HIV antibody and Hepatitis B infection. In molecular biology, it can detect the presence of phosphorylated proteins, tagged proteins or various isoforms of proteins. In genetics, it can be used for gene expression studies amongst others.

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DAB System-1610500011730

DAB System-1610500011730
₹ 6,380.00

Brand: Bangalore GeNei

Catalog No.: 1610500011730

Quantity/Unit: 10x6mg/Pack

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TMB/H2O2 for localization-1610380101730

TMB/H2O2 for localization-1610380101730
₹ 3,861.00

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Catalog No.: 1610380101730

Quantity/Unit: 10 ml/Pack

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BCIP/NBT-1610280501730

BCIP/NBT-1610280501730
₹ 3,949.00

Brand: Bangalore GeNei

Catalog No.: 1610280501730

Quantity/Unit: 50 ml/Pack

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Western Blot Development Kit for Human Antibody-2601300011730

Western Blot Development Kit for Human Antibody-2601300011730
₹ 14,234.00

Brand: Bangalore GeNei

Catalog No.: 2601300011730

Quantity/Unit: 10 blots/Kit

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Western Blot Development Kit for Mouse Antibody-2601400011730

Western Blot Development Kit for Mouse Antibody-2601400011730
₹ 14,234.00

Brand: Bangalore GeNei

Catalog No.: 2601400011730

Quantity/Unit: 10 blots/Kit

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Western Blot Development Kit for Rabbit Antibody-2601500011730

Western Blot Development Kit for Rabbit Antibody-2601500011730
₹ 14,234.00

Brand: Bangalore GeNei

Catalog No.: 2601500011730

Quantity/Unit: 10 blots/Kit

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Nitrocellulose Blotting Membrane, 15 X 15 cm, 0.22 um-SF107A-1x25NO

Nitrocellulose Blotting Membrane, 15 X 15 cm, 0.22 um-SF107A-1x25NO
₹ 5,300.00

Brand: HIMEDIA

Catalog No.: SF107A-1x25NO

Quantity/Unit: 1x25 no/PACK

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Nitrocellulose Blotting Membrane, 15 X 15 cm, 0.45 um-SF107B-1x25NO

Nitrocellulose Blotting Membrane, 15 X 15 cm, 0.45 um-SF107B-1x25NO
₹ 5,300.00

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Catalog No.: SF107B-1x25NO

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Nitrocellulose Blotting Membrane, 20 X 20 cm, 0.22 um-SF108A-1x25NO

Nitrocellulose Blotting Membrane, 20 X 20 cm, 0.22 um-SF108A-1x25NO
₹ 8,550.00

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Catalog No.: SF108A-1x25NO

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Nitrocellulose Blotting Membrane, 20 X 20 cm,0.45 um-SF108B-1x25NO

Nitrocellulose Blotting Membrane, 20 X 20 cm,0.45 um-SF108B-1x25NO
₹ 8,550.00

Brand: HIMEDIA

Catalog No.: SF108B-1x25NO

Quantity/Unit: 1x25 no/PACK

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Nitrocellulose Blotting Membrane, 30 X 30 cm, 0.22 um-SF109A-1X25NO

Nitrocellulose Blotting Membrane, 30 X 30 cm, 0.22 um-SF109A-1X25NO
₹ 12,825.00

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Catalog No.: SF109A-1X25NO

Quantity/Unit: 1x25 no/PACK

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Nitrocellulose Blotting Membrane, 30 X 30 cm, 0.45 um-SF109B-1X25NO

Nitrocellulose Blotting Membrane, 30 X 30 cm, 0.45 um-SF109B-1X25NO
₹ 12,825.00

Brand: HIMEDIA

Catalog No.: SF109B-1X25NO

Quantity/Unit: 1x25 no/PACK

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Nitrocellulose Blotting Membrane Roll, 30 cm X 3 m, 0.22 um-SF110A-1ROLL

Nitrocellulose Blotting Membrane Roll, 30 cm X 3 m, 0.22 um-SF110A-1ROLL
₹ 7,950.00

Brand: HIMEDIA

Catalog No.: SF110A-1ROLL

Quantity/Unit: 1 roll/PACK

Usually Shipped in: 2-3 Weeks

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Nitrocellulose Blotting Membrane Roll, 30 cm X 3 m, 0.45 um-SF110B-1ROLL

Nitrocellulose Blotting Membrane Roll, 30 cm X 3 m, 0.45 um-SF110B-1ROLL
₹ 7,950.00

Brand: HIMEDIA

Catalog No.: SF110B-1ROLL

Quantity/Unit: 1 roll/PACK

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Nylon-66 Blotting Membrane, 15 X 15 cm, 0.22 um-SF111A-1x25NO

Nylon-66 Blotting Membrane, 15 X 15 cm, 0.22 um-SF111A-1x25NO
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