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Western Blotting

Western Blotting, also known as immunoblotting, is a widely used method/technique to detect and/or quantify specific proteins from the sample of interest. The procedure involves a few stages. The protein sample is initially loaded and run on polyacrylamide gel electrophoresis wherein a voltage is applied and the protein separates based on their charge/mass ratio. A reference protein ladder is used to assess the molecular weight of proteins in the sample. The proteins are then transferred from the gel onto a membrane usually made of nitrocellulose or polyvinylidene difluoride (PVDF). This transfer is accomplished by electric current in electroblotting or by capillary action through the filter papers. A primary antibody binds to the protein of interest (now on the membrane). In some procedures, a secondary antibody is also used which binds to the primary antibody's Fc region. Various methods can be used for final detection of antibody bound protein of interest like Chemiluminescent detection or Fluorescent detection depending on the probe.
Western Blotting is a highly sensitive method for protein detection. In diagnostics, it is employed in detecting anti-HIV antibody and Hepatitis B infection. In molecular biology, it can detect the presence of phosphorylated proteins, tagged proteins or various isoforms of proteins. In genetics, it can be used for gene expression studies amongst others.

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BCIP/NBT-1610280501730

BCIP/NBT-1610280501730

₹ 3,590.00

Brand: Bangalore GeNei

Catalog No.: 1610280501730

Quantity/Unit: 50 ml/Pack

Usually Shipped in: 2-3 Weeks

DAB System-1610500011730

DAB System-1610500011730

₹ 5,800.00

Brand: Bangalore GeNei

Catalog No.: 1610500011730

Quantity/Unit: 10x6mg/Pack

Usually Shipped in: 2-3 Weeks

GeNei Northern Blotting Teaching Kit-6113800011730

GeNei Northern Blotting Teaching Kit-6113800011730

₹ 13,790.00

Brand: Bangalore GeNei

Catalog No.: 6113800011730

Quantity/Unit: 5 expts. /Kit

Usually Shipped in: 2-3 Weeks

GeNei Southern Hybridization Teaching Kit with ETS5-6109600031730

GeNei Southern Hybridization Teaching Kit with ETS5-6109600031730

₹ 32,020.00

Brand: Bangalore GeNei

Catalog No.: 6109600031730

Quantity/Unit: 5 expts. /Kit

Usually Shipped in: 2-3 Weeks

GeNei Southern Hybridization Teaching Kit-6109600011730

GeNei Southern Hybridization Teaching Kit-6109600011730

₹ 10,220.00

Brand: Bangalore GeNei

Catalog No.: 6109600011730

Quantity/Unit: 5 expts. /Kit

Usually Shipped in: 2-3 Weeks

GeNei Western Blotting Teaching Kit with ETS3-6102100031730

GeNei Western Blotting Teaching Kit with ETS3-6102100031730

₹ 41,000.00

Brand: Bangalore GeNei

Catalog No.: 6102100031730

Quantity/Unit: 5 expts. /Kit

Usually Shipped in: 2-3 Weeks

GeNei Western Blotting Teaching Kit-6102100011730

GeNei Western Blotting Teaching Kit-6102100011730

₹ 12,650.00

Brand: Bangalore GeNei

Catalog No.: 6102100011730

Quantity/Unit: 5 expts. /Kit

Usually Shipped in: 2-3 Weeks

GeNei Western Blotting Teaching Kit-6102100021730

GeNei Western Blotting Teaching Kit-6102100021730

₹ 29,100.00

Brand: Bangalore GeNei

Catalog No.: 6102100021730

Quantity/Unit: 20 expts. /Kit

Usually Shipped in: 2-3 Weeks

HRP Conjugate Stabilizer(Diluent) - 100ml

HRP Conjugate Stabilizer(Diluent) - 100ml

10% Off
₹ 5,400.00 ₹ 6,000.00

Brand: Invitrosense

Catalog No.: EST-HCS-100ml

Quantity/Unit: 100ml / Pack

Usually Shipped in: 1-2 Weeks

HRP Conjugate Stabilizer(Diluent) - 1L

HRP Conjugate Stabilizer(Diluent) - 1L

10% Off
₹ 31,500.00 ₹ 35,000.00

Brand: Invitrosense

Catalog No.: EST-HCS-1L

Quantity/Unit: 1000ml / Pack

Usually Shipped in: 1-2 Weeks

HRP Conjugate Stabilizer(Diluent) - 500ml

HRP Conjugate Stabilizer(Diluent) - 500ml

10% Off
₹ 20,250.00 ₹ 22,500.00

Brand: Invitrosense

Catalog No.: EST-HCS-500ml

Quantity/Unit: 500ml/ Pack

Usually Shipped in: 1-2 Weeks

Immunoblot (TMB Monoblue) - 100ml

Immunoblot (TMB Monoblue) - 100ml

₹ 6,000.00

Brand: Invitrosense

Catalog No.: IBS-TMBY-100ml

Quantity/Unit: 100ml / Pack

Immunoblot (TMB Monoblue) - 1L

Immunoblot (TMB Monoblue) - 1L

₹ 35,000.00

Brand: Invitrosense

Catalog No.: IBS-TMBY-1L

Quantity/Unit: 1000ml / Pack

Immunoblot (TMB Monoblue) - 500ml

Immunoblot (TMB Monoblue) - 500ml

₹ 15,000.00

Brand: Invitrosense

Catalog No.: IBS-TMBY-500ml

Quantity/Unit: 500ml/ Pack

Immunoblot (TMB Ultra Blue) - 100ml

Immunoblot (TMB Ultra Blue) - 100ml

10% Off
₹ 5,400.00 ₹ 6,000.00

Brand: Invitrosense

Catalog No.: IBS-DB-100ml

Quantity/Unit: 100ml / Pack

Usually Shipped in: 1-2 Weeks