Autotaxin (ATX) is a secreted lysophospholipase D that catalyzes the hydrolysis of lysophosphatidylcholine (LPC) to generate lysophosphatidic acid (LPA). LPA is a lipid mediator that activates G protein-coupled receptors and induces various cellular responses.1 ATX-LPA signaling is involved in a range of pathologies including tumor progression and inflammation.2 ATX is proteolytically processed by clEAvage of a 35 amino acid signal peptide and then secreted as a mature protein.3 N-glycosylation of ATX at Asn53 and Asn410 is essential for activity and secretion of ATX from adipocytes.1 ATX is organized in a modular domain arrangement with a central phosphodiesterase catalytic site, which is similar to the catalytic domain of alkaline phosphatases.4 Two cysteine-rich somatomedin B-like domains (involved in protein-protein interactions) are located at the N-terminal portion of the protein followed by an adjacent hydrophobic-containing RGD tripeptide motif that may be involved in cell-extracellular matrix interactions. Finally, a nuclEAse-like domain is located at the C-terminus, which is catalytically inert and possibly involved in substrate binding and presentation.5 Technical Information Synonyms Ectonucleotide Pyrophosphatase/Phosphodiesterase-2 ENPP-2 Lysophospholipase D Purity 90% estimated by SDS-PAGE Source recombinant C-terminal histidine-tagged protein expressed using a baculovirus overexpression system in Sf21 cells MW 98.8 kDa Formulation 50 mM Tris-HCl, pH 8.0, containing 150 mM sodium chloride and 20% glycerol UniProt Accession Q13822-1