InSite PCR-Based Site-Directed Mutagenesis Kit is a fast, easy and efficient PCR based method to introduce point mutations as well as insertion and deletion mutations, an invaluable technique for protein structure-function and other related studies. The protocol involves two steps of PCR reactions, the product of the first PCR being used as the primer for second PCR. A PCR primer (supplied) that introduces a unique restriction site, is used for second PCR thus facilitating screening for mutants. The kit contains -Vector for subcloning, has multicloning sites similar to pUC 18, Primer I containing unique Restriction enzyme site, XT-5 PCR System, dNTP mix, Not I restriction enzyme with assay buffer. The kit can be used for Generating point mutation, insertion and deletion, Switch amino acids, delete or insert single or multiple amino acids.