HIS-SELECT(R) NICKEL AFFINITY GEL is supplied by Sigma-Aldrich. It is an immobilized metal ion affinity chromatography (IMAC) product, used for the purification of His-tagged proteins. While the unique, non-charged, hydrophilic linkage of the proprietary quadridentate NTA chelate group to the beaded agarose charged with nickel ensures high selectivity for small to medium scale His-tag protein purification, it also results in reduced non-specific binding of other proteins. It is selective for recombinant proteins with His-tags and exhibits low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography.

PRODUCT SPECIFICATIONS:

• Name: HIS-SELECT(R) NICKEL AFFINITY GEL
• Category: Protein Electrophoresis Biochemicals
• Brand: Sigma-Aldrich
• Pack size: 5ML
• Catalog number: P6611-5ML
• List price: 10800.3

PRODUCT FEATURES:

• Form: 1:1 suspension in a 20% ethanol solution
• Feature: hydrophilic
• Concentration: 1.5-2.4 mL/mL (suspension in packed gel)
• Color: faint blue to very dark blue
• Matrix: 6