Interferon regulatory factor 3 (IRF3) is a member of the IRF family. It plays a crucial role in activation of innate immunity and inflammation in response to viral infection, functioning as a molecular switch for antiviral activity.1,2,3,4,5 Double-stranded RNA generated during a viral infection lEAds to IRF3 activation by serine/threonine phosphorylation by TBK1 or IKBKE kinases, which induces a conformational change lEAding to its dimerization, nuclEAr localization, and association with CREBBP.1,2,3,6 The complex, known as DRAF1, activates transcription interferons alpha and beta, as well as other interferon-induced genes.1,6 These genes play a critical role in type 1 interferon (IFN)-dependent immune response.5 A recent study shows phosphorylation of innate immune adaptor proteins MAVS, STING, and TRIF recruits and specifies IRF3 phosphorylation and activation by the serine/threonine-protein kinase TBK1, thereby inducing the production of type-1 interferons.2,4,7Technical InformationSynonymsInterferon Regulatory Factor 3Purity85% as estimated by SDS-PAGESourceN-terminally Histidine-tagged human IRF3 protein (full length) purified from E. coliAmino Acids1-427 (full length)MW49.6 kDaFormulation50 mM HEPES, pH 8.0, 150 mM sodium chloride, and 10% glycerolUniProt Accession Q14653