Myco-Sniff Mycoplasma PCR Detection Kit has been demonstrated to be a highly sensitive, specific and rapid method for the detection of mycoplasma contamination in cell cultures. This kit greatly simplifies testing and detection of mycoplasma contamination in cell cultures with results in less than 3 hours. After PCR, the presence of contaminating mycoplasma can be easily and sensitively detected by simply verifying the bands of amplified DNA fragments after gel electrophoresis. Myco-Sniff Mycoplasma PCR Detection Kit can be used to detect a broader range of mycoplasma species compared to other commercially available PCR-based mycoplasma detection kits. The unique and specific primer sets used in the kit were designed from DNA sequences that code for highly conserved 16S rRNA from relevant mycoplasma species, thus preventing amplification of DNA from other sources. The high sensitivity of the kit allows for the detection of as little as 20 CFU/mL.