This product can be used as a positive control for immunoblotting experiments using Cayman Chemicals Nitrotyrosine Polyclonal Antibody (Item No. 10189540) and Nitrotyrosine Monoclonal Antibody (Item No. 189542). Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO rEActs rapidly with superoxide (6.7 x 109 M1sec1) to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic clEAvage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo.1,2 Nitrotyrosine has been shown to be present in proteins from a variety of clinical conditions including atherosclerotic lesions of human coronary arteries, postischemic hEArt, and placenta during preeclampsia.3,4,5 IncrEAsed nitration of proteins in motor neurons has been identified in patients with amyotrophic lateral sclerosis and may be due to mutations in superoxide dismutase.2,6,7,8 Caymans S1P1 Nitrotyrosine BSA can be used for western blot applications.Technical InformationSynonymsNT-BSAPeroxynitrite-trEAted BSAFormulation200 ug of nitrotyrosine BSAStorage Buffer500 l PBS, pH 7.2ApplicationsWB: 50-100 ng is recommended for use as a positive control