Overview Product Name Nuclear Fast Red SKU/Catalog Number AR0008 Pack Size 12 mL(for 240 assays) Form Supplied Solution Format Ready-to-Use Concentration 0.1% Nuclear Fast Red in distilled water Appearance Red Storage Upon receipt store at 4C for one year. Application Counterstain in IHC (BCIP/NBT chromogenic reaction) Equivalent Vector Labs (Product No. H-3403), Millipore Sigma (Product No. N 8002) Description Bosters Nuclear Fast Red is a rapid staining solution which is a popular alternative to hematoxylin and typically stains nuclei in five minutes. Cite This Product Nuclear Fast Red (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0008) Chemical structuresMolecular formula: C14H8NNaO7SMolecular weight: 357.28CAS Number: 6409-77-4BackgroundNuclear Fast Red is suitable for the determination of calcium. It is suitable for use in prussian blue staining in neural progenitor cells. It may be employed as highly sensitive "off/on" fluorescent probe for the determination of guanine. Nuclear fast red (2-anthracenesulfonic acid, 4-amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-, monosodium salt) may be used as selective complexing agent for the simultaneous determination of copper and bismuth by adsorptive stripping voltammetry. Bosters Nuclear Fast Red is an optimized nuclear counterstain solution that produces an intense nuclear stain. It is an anionic dye used in conjunction with an aluminium salt as a mordant. In contrast to hematoxylin, Nuclear Fast Red is a rapid staining procedure, which requires only step and stains nucleus in five minutes. It is an excellent alternative in multiple labeling when hematoxylin would otherwise obscure the substrate colors.Protocol1.Rinse sections in distilled water.2.Add 50 l Nuclear Fast Red directly onto the tissue section and incubate for 5 minutes.3.Wash with distilled water for 1 minute.