Versene (EDTA) 0.1% Solution 1X is supplied by HiMedia. This product contains 0.1% Versene in Dulbeccos Phosphate Buffered Saline. It is available in the form of a colourless, clear solution with a pH in the range of 6.7 to 7.3 and an osmolality between 270.00 -310.00 mOsm/Kg H2O. It is advisable to store this product between 2-8C.
Versene (Ethylenediaminetetraacetic acid or EDTA) is a chelating agent that binds divalent cations such as calcium and magnesium. Chelation of these cations leads to dissociation of cell monolayers and release of the cells into suspension. Versene can be used as a wash prior to addition of trypsin or in combination with trypsin. The combination works faster than trypsin or versene alone because versene allows trypsin to hydrolyze specific intercellular peptide bonds and destabilize the intercellular matrix.