PCR
Polymerase chain reaction or PCR is a technique developed by American biochemist Kary Mullis to amplify a DNA of interest. In a conventional PCR reaction, the first step is the heat-denaturation of the double-stranded DNA at 94 to 96C. This is followed by annealing of the specifically designed primer at the target site and then polymerase catalyzed elongation by the addition of the nucleotides. The denaturation takes about 30 secs, the annealing about 20 to 40 secs and the elongation takes several minutes depending upon the number of cycles set in the PCR machine. PCR machine, DNA template, a polymerase enzyme (usually the heat-resistant Taq polymerase), specifically designed forward and reverse primers, the deoxynucleoside triphosphates or dNTPs mixture which will provide the nucleotides for elongation - they act as the building blocks of the new strand, and the buffer solution to maintain the pH, are the key requirements. The reaction is carried out in 0.1 ml / 0.2 ml PCR tubes or specially designed PCR plates. PCR Kits are also available with everything readymade and quick to use.
In addition to the conventional PCR, a gradient PCR with a range of variation in annealing temperature can also be used. It is Ideal when while developing the protocol for a new DNA sample. Quantitative PCR (qPCR) which uses fluorescent dyes like Sybr Green can be performed to measure the quantity of the amplified products in real-time.
96-well PCR plate, non-skirted, low profile, transparent, blue coding
- Brand:
BRAND - Catalog No.:
781366 - Quantity/Unit: 50 pieces/Pack
- Usually Shipped in: 5-6 Weeks
96-well PCR plate, non-skirted, low profile, white, blue coding
- Brand:
BRAND - Catalog No.:
781367 - Quantity/Unit: 50 pieces/Pack
- Usually Shipped in: 5-6 Weeks
96-well PCR plate, non-skirted, standard profile, transparent, blue coding
- Brand:
BRAND - Catalog No.:
781368 - Quantity/Unit: 50 pieces/Pack
- Usually Shipped in: 5-6 Weeks
96-well PCR plate, non-skirted, standard profile, white, blue coding
- Brand:
BRAND - Catalog No.:
781369 - Quantity/Unit: 50 pieces/Pack
- Usually Shipped in: 5-6 Weeks
96-well PCR plate, semi-skirted, low profile, transparent, blue coding
- Brand:
BRAND - Catalog No.:
781371 - Quantity/Unit: 50 pieces/Pack
- Usually Shipped in: 5-6 Weeks
96-well PCR plate, semi-skirted, low profile, white, blue coding
- Brand:
BRAND - Catalog No.:
781372 - Quantity/Unit: 50 pieces/Pack
- Usually Shipped in: 5-6 Weeks
96-well PCR plate, semi-skirted, raised skirt, low profile, blue coding, transparent
- Brand:
BRAND - Catalog No.:
781373 - Quantity/Unit: 50 pieces/Pack
- Usually Shipped in: 5-6 Weeks
96-well PCR plate, semi-skirted, raised skirt, low profile, white, blue coding
- Brand:
BRAND - Catalog No.:
781374 - Quantity/Unit: 50 pieces/Pack
- Usually Shipped in: 5-6 Weeks
96-well PCR plate, semi-skirted, standard profile, transparent, blue coding
- Brand:
BRAND - Catalog No.:
781375 - Quantity/Unit: 50 pieces/Pack
- Usually Shipped in: 5-6 Weeks
96-well PCR plate, semi-skirted, standard profile, white, blue coding
- Brand:
BRAND - Catalog No.:
781376 - Quantity/Unit: 50 pieces/Pack
- Usually Shipped in: 5-6 Weeks
96-well PCR plate, skirted, low profile, transparent, black coding
- Brand:
BRAND - Catalog No.:
781377 - Quantity/Unit: 50 pieces/Pack
- Usually Shipped in: 5-6 Weeks
96-well PCR plate, skirted, low profile, white, black coding
- Brand:
BRAND - Catalog No.:
781378 - Quantity/Unit: 50 pieces/Pack
- Usually Shipped in: 5-6 Weeks
96-well PCR plate, semi-skirted, standard profile, elevated rim, transparent
- Brand:
BRAND - Catalog No.:
781400 - Quantity/Unit: 50 pieces/Pack
- Usually Shipped in: 5-6 Weeks
24-well PCR-plates, no skirt, PP, white, 0.2 ml, for qPCR, BIO-CERT PCR QUALITY
- Brand:
BRAND - Catalog No.:
781412 - Quantity/Unit: 40 pieces/Pack
- Usually Shipped in: 5-6 Weeks
48-well PCR-plates, no skirt, PP, transparent, 0.2 ml, for qPCR, BIO-CERT PCR QUALITY
- Brand:
BRAND - Catalog No.:
781415 - Quantity/Unit: 20 pieces/Pack
- Usually Shipped in: 5-6 Weeks
Filmstrips polyester, self adhesive, for PCR, qPCR and storage
- Brand:
BRAND - Catalog No.:
781383 - Quantity/Unit: 50 pieces/Pack
- Usually Shipped in: 5-6 Weeks
Sealing film, Polyester, self-adhesive, for ELISA and PCR
- Brand:
BRAND - Catalog No.:
781390 - Quantity/Unit: 100 pieces/Pack
- Usually Shipped in: 5-6 Weeks
Ground joint sleeves, PTFE, for NS 29/32, thin-walled
- Brand:
BRAND - Catalog No.:
51422 - Quantity/Unit: 10/Pack
- Usually Shipped in: 5-6 Weeks
Ground socket, PTFE NS 29/32, grip collar and sealing rings
- Brand:
BRAND - Catalog No.:
51467 - Quantity/Unit: 1/Pack
- Usually Shipped in: 5-6 Weeks
48-well PCR-plates, no skirt, PP, white, 0.2 ml, for qPCR, BIO-CERT PCR QUALITY
- Brand:
BRAND - Catalog No.:
781416 - Quantity/Unit: 20 pieces/Pack
- Usually Shipped in: 5-6 Weeks
One Step RT PCR Kit
- Brand:
BR Biochem Life Sciences - Catalog No.:
BC9K-005- 0011 - Quantity/Unit: 100Rxn/Pack
- Usually Shipped in: 3-4 Weeks
One Step MultiPlex qRTPCR Kit with Probe
- Brand:
BR Biochem Life Sciences - Catalog No.:
BC9K-005- 0012 - Quantity/Unit: 100Rxn/Pack
- Usually Shipped in: 3-4 Weeks
NTP Mixture (10mM)-BC0056
- Brand:
BR Biochem Life Sciences - Catalog No.:
BC0056 - Quantity/Unit: 0.5ml/Vial
- Usually Shipped in: 3-4 Weeks
NTP Mixture (25mM)-BC0057
- Brand:
BR Biochem Life Sciences - Catalog No.:
BC0057 - Quantity/Unit: 0.5ml/Vial
- Usually Shipped in: 3-4 Weeks
NTP Set Solutions (100mM each)-BC0059
- Brand:
BR Biochem Life Sciences - Catalog No.:
BC0059 - Quantity/Unit: 4X0.25ml/Pack
- Usually Shipped in: 3-4 Weeks
PCR Purification Kit- MPK4
- Brand:
Aura Biotech - Catalog No.:
ABT-172S - Quantity/Unit: 20 Rxns/Pack
- Usually Shipped in: 4-7 Days
PCR Purification Kit- MPK6
- Brand:
Aura Biotech - Catalog No.:
ABT-172XL - Quantity/Unit: 250 Rxns/Pack
- Usually Shipped in: 4-7 Days
2X Taq DNA PCR Master Mix RED (1.25 ml), 50 Rxns
- Brand:
Aura Biotech - Catalog No.:
ABT-011S - Quantity/Unit: 50 Reactions/Pack
- Availability: In stock