RNA Purifications and Extractions
RNA purification and extraction is the process of isolating RNA samples from biological extracts. There are many procedures for RNA extraction, the most common of which is Guanidinium thiocyanate-phenol-chloroform extraction. This is aliquidliquidextraction method which employs Guanidinium thiocyanate to denature proteins present in the sample. RNA partitions into the aqueous phase of the mixture. The phases are produced by phenol and chloroform in the solution.
However, the process is complicated by the universal presence of ribonuclease enzymes in cells and tissues, which rapidly degrade RNA. For this reason, equipment used in RNA extraction procedures is thoroughly cleaned and treated with harsh chemicals to destroy RNases. Another method of RNA extraction in liquid nitrogen, using a mortar and pestle (or tissue pulverizers) is also efficient in preventing ribonuclease activity.
Based on the requirements of researchers, various products are available. These include guanidine thiocyanate reagents, yeast RNA purification kits, plant RNA extraction kits etc., available from brands such as Fisher BioReagents, Finnzymes, HiMedia on Biomall.in
NucleoSpin RNA Stool (10)
- Brand:
Macherey Nagel Germany - Catalog No.:
740130.10 - Quantity/Unit: 10 preps/Box
NucleoSpin RNA Stool (50)
- Brand:
Macherey Nagel Germany - Catalog No.:
740130.50 - Quantity/Unit: 50 preps/Box
NucleoBond RNA Soil (20)
- Brand:
Macherey Nagel Germany - Catalog No.:
740140.20 - Quantity/Unit: 20 preps/Box
NucleoBond RNA Soil Mini (10)
- Brand:
Macherey Nagel Germany - Catalog No.:
740142.10 - Quantity/Unit: 10 preps/Box
NucleoBond RNA Soil Mini (50)
- Brand:
Macherey Nagel Germany - Catalog No.:
740142.50 - Quantity/Unit: 50 preps/Box
NucleoSpin RNA Blood (10)
- Brand:
Macherey Nagel Germany - Catalog No.:
740200.10 - Quantity/Unit: 10 preps/Box
NucleoSpin RNA Blood (50)
- Brand:
Macherey Nagel Germany - Catalog No.:
740200.50 - Quantity/Unit: 50 preps/Box
NucleoSpin RNA Blood Midi (20)
- Brand:
Macherey Nagel Germany - Catalog No.:
740210.20 - Quantity/Unit: 20 preps/Box
NucleoSpin RNA XS (10)
- Brand:
Macherey Nagel Germany - Catalog No.:
740902.10 - Quantity/Unit: 10 preps/Box
NucleoSpin RNA XS (250)
- Brand:
Macherey Nagel Germany - Catalog No.:
740902.250 - Quantity/Unit: 250 preps/Box
NucleoSpin RNA XS (50)
- Brand:
Macherey Nagel Germany - Catalog No.:
740902.50 - Quantity/Unit: 50 preps/Box
NucleoSpin RNA/Protein (10)
- Brand:
Macherey Nagel Germany - Catalog No.:
740933.10 - Quantity/Unit: 10 preps/Box
NucleoSpin RNA/Protein (250)
- Brand:
Macherey Nagel Germany - Catalog No.:
740933.250 - Quantity/Unit: 250 preps/Box
NucleoSpin RNA/Protein (50)
- Brand:
Macherey Nagel Germany - Catalog No.:
740933.50 - Quantity/Unit: 50 preps/Box
NucleoSpin RNA Plant (10)
- Brand:
Macherey Nagel Germany - Catalog No.:
740949.10 - Quantity/Unit: 10 preps/Box
NucleoSpin RNA Plant (250)
- Brand:
Macherey Nagel Germany - Catalog No.:
740949.250 - Quantity/Unit: 250 preps/Box
NucleoSpin RNA Plant (50)
- Brand:
Macherey Nagel Germany - Catalog No.:
740949.50 - Quantity/Unit: 50 preps/Box
NucleoSpin RNA (10)
- Brand:
Macherey Nagel Germany - Catalog No.:
740955.10 - Quantity/Unit: 10 preps/Box
NucleoSpin RNA (250)
- Brand:
Macherey Nagel Germany - Catalog No.:
740955.250 - Quantity/Unit: 250 preps/Box
NucleoSpin RNA (50)
- Brand:
Macherey Nagel Germany - Catalog No.:
740955.50 - Quantity/Unit: 50 preps/Box
NucleoSpin RNA Midi (20)
- Brand:
Macherey Nagel Germany - Catalog No.:
740962.20 - Quantity/Unit: 20 preps/Box
NucleoSpin TriPrep (10)
- Brand:
Macherey Nagel Germany - Catalog No.:
740966.10 - Quantity/Unit: 10 preps/Box
NucleoSpin TriPrep (250)
- Brand:
Macherey Nagel Germany - Catalog No.:
740966.250 - Quantity/Unit: 250 preps/Box
NucleoSpin TriPrep (50)
- Brand:
Macherey Nagel Germany - Catalog No.:
740966.50 - Quantity/Unit: 50 preps/Box
NucleoSpin totalRNA FFPE XS (10)
- Brand:
Macherey Nagel Germany - Catalog No.:
740969.10 - Quantity/Unit: 10 preps/Box
NucleoSpin totalRNA FFPE XS (250)
- Brand:
Macherey Nagel Germany - Catalog No.:
740969.250 - Quantity/Unit: 250 preps/Box
NucleoSpin totalRNA FFPE XS (50)
- Brand:
Macherey Nagel Germany - Catalog No.:
740969.50 - Quantity/Unit: 50 preps/Box
NucleoSpin RNA Plant and Fungi (250)
- Brand:
Macherey Nagel Germany - Catalog No.:
740120.250 - Quantity/Unit: 250 preps/Box