DMEM, Low glucose w/ 1g/L Glucose, L-Glutamine, Sodium pyruvate w/o NaHCO3, Phenol red

DMEM, Low glucose w/ 1g/L Glucose, L-Glutamine, Sodium pyruvate w/o NaHCO3, Phenol red

₹ 4,200.00 *

Brand: HIMEDIA

Catalog No.: AT183-20L

Quantity/Unit: 20lt/EA

Usually Shipped in: 2-3 Weeks

Qty:
Min Orderable Qty : 1 Pack

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Product Description

Dulbecco's Modified Eagle Medium (DMEM) With 1gm Glucose per litre, L-Glutamine, Sodium pyruvate Without Sodium bicarbonate and Phenol red is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.7 -6.3 whereas the pH with Sodium Bicarbonate is between 7.2-7.8. The osmolality without Sodium Bicarbonate is in the range of 230.00 -270.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 310.00 -350.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Calcium chloride dihydrate (265.00)

• Ferric nitrate nonahydrate (0.10)

• Magnesium sulphate anhydrous (97.72)

• Potassium chloride (400.00)

• Sodium chloride (6400.00)

• Sodium dihydrogen phosphate anhydrous (109.00)

• Amino acids

• Choline chloride (4.00)

• D-Ca-Pantothenate (4.00)

• Folic acid (4.00)

• Nicotinamide (4.00)

• Pyridoxal hydrochloride (4.00)

• Riboflavin (0.400)

• Thiamine hydrochloride (4.00) 

• i-Inositol (7.20)

• D-Glucose (1000.00)

• Sodium pyruvate (110.00)

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of Glucose and was originally used to culture embryonic mouse cells. This product contains 1gm glucose per litre, L-glutamine and sodium pyruvate but does not contain phenol red. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.

 

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