RPMI-1640 w/o Folic acid, L-Glutamine and Sodium bicarbonate-AT161A-1L

RPMI-1640 w/o Folic acid, L-Glutamine and Sodium bicarbonate-AT161A-1L

₹ 510.00 *

Brand: HIMEDIA

Catalog No.: AT161A-1L

Quantity/Unit: 1lt/EA

Usually Shipped in: 2-3 Weeks

Qty:
Min Orderable Qty : 1 Pack

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For lab/research use only, unless otherwise specified

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Product Description

RPMI-1640 Without Folic Acid, L-Glutamine and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 7.2 -7.8 whereas the pH with Sodium Bicarbonate is between 7.4 -8.0. The osmolality without Sodium Bicarbonate is in the range of 230.00 -270.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 280.00 -320.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Calcium nitrate tetrahydrate (100.00)

• Disodium hydrogen phosphate anhydrous (800.00)

• Magnesium sulphate anhydrous (48.84)

• Potassium chloride (400.00)

• Sodium chloride (6000.00)

• Amino acids

• Choline chloride (3.00)

• D-Biotin (0.20)

• D-Ca-Pantothenate (0.25)

• Niacinamide (1.00)

• Pyridoxine hydrochloride (1.00)

• Riboflavin (0.20)

• Thiamine hydrochloride (1.00)

• Vitamin B12 (0.005)

• i-Inositol (35.00)

• p-Amino benzoic acid (PABA) (1.00)

• D-Glucose (2000.00)

• Glutathione reduced (1.00)

• Phenol red sodium salt (5.30)

Roswell Park Memorial Institute (RPMI) media are a series of media developed by Moore et al for the culture of human normal and neoplastic cells in vitro. RPMI 1640 is the most commonly used medium in the series. A modification of McCoy's 5A medium, the medium was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Presently the medium is extensively used for a wide range of anchorage dependant cell lines. The medium needs to be supplemented with 5-20

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