Alternative Thioglycollate Medium (NIH Thioglycollate Broth)
Catalog No.: DM264-100g
Usually Shipped in: 1-2 Weeks
For lab/research use only, unless otherwise specified
- Product Details
High grade Alternative Thioglycollate Medium (NIH Thioglycollate Broth) is supplied by Micromaster. It is available in the form of a cream to yellow homogenous free flowing powder with a pH in the range of 6.9 to 7.3. This product should be stored between 10-30°C in a tightly closed container. Once opened, it should be properly stored, after tightly capping the bottle. Due to the hygroscopic nature of the product, there could be chances of lump formation which can be avoided by proper storage. Store in dry ventilated area protected from extremes of temperature and sources of ignition.
- Quantity provided: 100 g/pack
Alternative Thioglycollate Medium is formulated as described in the N.I.H. memorandum. It is used for the sterility testing of certain biological products which are turbid or viscous and cannot be tested using Fluid Thioglycollate Medium. Both the media have similar composition, except agar and resazurin that are not included in Alternative Thioglycollate Medium. This deletion makes it suitable for sterility testing of viscous products. Tryptone serves as a source of nitrogen and carbon compounds, long chain amino acids and other essential nutrients. Yeast extract serve as source of essential nutrients to the contaminants, if present. Dextrose serves as the energy source. Sodium chloride maintains the osmotic equilibrium of the medium whereas L-cystine, an amino acid, also serves as source of essential growth factors. Sodium thioglycollate and L-cystine lower the oxidation-reduction potential of the medium by removing oxygen to maintain a low Eh. Sodium thioglycollate also helps to neutralize the toxic effects of mercurial preservatives.