High grade Antibiotic Assay Medium No. 3 (Assay Broth) is supplied by Micromaster. It is available in the form of a cream to yellow homogeneous free flowing powder which has a pH in the range of 6.8 to 7.2. Peptic digest of animal tissue, beef extract and yeast extract provide essential nutrients and growth factors for enhanced microbial growth. Sodium chloride maintains the osmotic equilibrium of the medium and retains the cell viability and cell integrity. Phosphates in the medium provide good buffering action. Dextrose serves as the carbon and energy source. This product should be stored between 30C in a tightly closed container. Store in dry ventilated area protected from extremes of temperature and sources of ignition.

Quantity provided: 100 g/pack

Antibiotic Assay Medium is used in the performance of antibiotic assays. Grove and Randall have elucidated those antibiotic assays and media in their comprehensive treatise on antibiotic assays. Antibiotic Assay Medium No. 3 (Assay Broth) is used in the microbiological assay of different antibiotics in pharmaceutical and food products by the turbidimetric method. Turbidimetric antibiotic assay is based on the change or inhibition of growth of a test microorganism in a liquid medium containing a uniform concentration of an antibiotic. After incubation of the test organism in the working dilutions of the antibiotics, the amount of growth is determined by measuring the light transmittance using spectrophotometer. The concentration of antibiotic is determined by comparing amounts of growth obtained with that given by the reference standard solutions. Use of this method is appropriate only when test samples are clear.