High grade Antibiotic Assay Medium No. 1 (Seed Agar) is supplied by Micromaster. It is available in the form of a cream to yellow homogeneous free flowing powder which has a pH in the range of 6.4 to 6.8. Nutrients and growth factors are supplied by the ingredients like peptone, Tryptone, yeast extract and HM Peptone B. Dextrose is supplemented as a carbon and energy source. This product should be stored between 10-30C in a tightly closed container. Once opened, it should be properly stored, after tightly capping the bottle. Due to the hygroscopic nature of the product, there could be chances of lump formation which can be avoided by proper storage. Store in dry ventilated area protected from extremes of temperature and sources of ignition.

Quantity provided: 500 g/pack

The potency of an antibiotic can be determined by chemical, physical and biological means. An assay is made to. Biological tests offer the most convenient means of performing an assay, which determine the ability of an antibiotic to kill or inhibit the growth of living microorganisms. Antibacterial susceptibility testing may be performed by either dilution (turbidimetric) or diffusion methods. The choice of methodology is often based on many factors, including relative ease of performance, flexibility and use of automated or semi-automated devices for both identification and susceptibility testing. Antibiotic Assay Medium No.1 is used in the microbiological assay of -lactam and other antibiotics. This media is prepared according to the specifications detailed in various pharmacopoeias and by the FDA. Test organisms are inoculated in sterile seed agar cooled to 40-45C and spread evenly over the surface of solidified base agar. After incubation the concentration of the antibiotic being assayed is determined by measuring the zone of inhibition obtained, with that of reference standard antibiotic.